GPMDB00335OtherZeiler M, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mousemmann@biochem.mpg.de25205226Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistryhttp://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017734http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017728http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017754http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017741Not availableGPMDBKnowledge of the identity and quantity of expressed proteins of a cell type is a prerequisite for a complete understanding of its molecular functions. Mass-spectrometry-based proteomics has allowed the identification of the entire protein complement of yeast and the close-to-complete set of proteins expressed in mammalian cell lines. Using recent technological advances, we here characterized the proteome of murine platelets, key actors in mediating hemostasis and thrombosis. We accurately measured the absolute protein concentrations of 13 platelet proteins using SILAC-protein epitope signature tags and used them as reference points to estimate the copy numbers of all proteins of the platelet proteome. To distinguish contaminants such as plasma or erythrocyte proteins from true platelet proteins, we monitored protein abundance profiles across multiple purification steps. In total, we absolutely quantified 4,400 platelet proteins, with estimated copy numbers ranging from less than 10 to about a million per cell. Stoichiometries derived from our data correspond well with previous studies. Our study provides a close-to-complete reference map of platelet proteins that will be useful to the community, for instance, for interpreting mouse models of human platelet diseases.Copy number analysis of the murine platelet proteome spanning the complete abundance range.Zeiler Marlis M,Moser Markus M,Mann Matthias M,Zeiler Marlis M, Moser Markus M, Mann Matthias Mdetermination, Blood, number, Blood Platelet, extra or missing physical or functional parts, number of, enucleate thrombocyte, presence, mereological quality, blood platelets, count in organism, count, Thrombocytes, anucleate thrombocyte, has or lacks parts of type, cardinality, chemical analysis, Proteomes., thrombocyte, Platelet, blood platelet, assay, quantitative, Thrombocyte, platelet, Platelets, presence or absence in organismdata, Thrombus, 10q23del, PLATEST, mol, GLM2, cell, MMAC1, Clot, Blood, Blood Clots, Blood Clot., Estimated, Cell, Thromboses, MHAM, DEC, Experiment, Clots, Hemostases, PTEN1, CWS1, TEP1, BZS, Proteomes, Plateletsdetermination, Blood, number, Blood Platelet, extra or missing physical or functional parts, number of, enucleate thrombocyte, presence, mereological quality, blood platelets, count in organism, count, Thrombocytes, anucleate thrombocyte, has or lacks parts of type, cardinality, chemical analysis, Proteomes., thrombocyte, Platelet, blood platelet, assay, quantitative, Thrombocyte, platelet, Platelets, presence or absence in organismFresh, 3.4.22.-, IPP2A2, Thrombus, human being, Fresh Frozen Plasma, Laboratory, Clot, Blood, Mus domesticus, RBC, FBN, Gene, baker's yeast, mini-ICE, CASP-14, Homo sapiense, House Mouse, Saccharomyces italicus, PHAPII, 5730420M11Rik, Thromboses, Readability, Homo spaiens, Homo sapien, Thrombocytes, template-activating factor I, House, ECTOL1, Homo sapians, yeast, Gene Products, Line, thrombocyte, Mus musculus domesticus, Analysis, Mice, WMS, lager beer yeast, MAP, study, Set, SET, Fresh Frozen Plasmas, Fresh Frozen, rabGAPLP, Blood Plasma, Homo sapients, Identification, TAF-I, red blood corpuscle, cell, Swiss, Sets (Psychology), Spectrum, phosphatase 2A inhibitor I2PP2A, Saccharomyes cerevisiae, cell line cell, RabGAP-5, Swiss Mice, proteins, Saccharomyces uvarum var. melibiosus, Estimated, "mouse" EXACT common_name [], man, cell line, OCTD, "Mus muscaris" RELATED misnomer [], IGAAD, Candida robusta, 2PP2A, anucleate thrombocyte, Homo sapience, Saccharomyces capensis, Concentration, RUSC3, Homo sampiens, Concentrations, Blood Plasmas, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], house mouse, brewer's yeast, GPHYSD2, Thrombocyte, Home sapiens, plasma, SGS, Blood Clot, data, Frozen Plasma, MUTYH-Associated Polyposis, PLATEST, Proteins, Blood Platelet, mouse, Cell Lines, Blood Clots, StF-IT-1, cell_line, Plasmas, Saccaromyces cerevisiae, Cell, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, ACMICD, Spectroscopy, polypeptide, Sccharomyces cerevisiae, Frozen Plasmas, Hemostases, I-2PP2A, Mus, Homo sapian, Diseases., Protein, Mini-ICE, I2PP2A, MFS1, Homo sapeins, WMS2, Lines, Saccharomyces oviformis, Plasma, Mus musculus, Yeast, RUTBC3, Epistemology, portion of blood plasma, HLA-DR-associated protein II, Caspase-14 subunit p10, mice, red blood cell, Swiss Mouse, Spectrometry, Humo sapiens, Identifications (Psychology), portion of plasma, House Mice, Caspase-14 subunit p19, MASS, Understanding, inhibitor of granzyme A-activated DNase, enucleate thrombocyte, MYH-Associated Polyposis, MICE, Homo sapines, cell_type, human, RABGAP5, Laboratory Mice, domesticus, Protein Gene Products, "house mouse" EXACT genbank_common_name [], Gene Proteins, blood platelets, blood plasm, 2610030F17Rik, Homo spiens, "human" EXACT genbank_common_name [], TAF-IBETA, Clots, SSKS, Platelet, blood platelet, Mouse, AA407739, platelet, Proteomes, Attentions, Laboratory Mouse, Platelets3305http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017734dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0101789019123997540.01.0falseCopy number analysis of the murine platelet proteome spanning the complete abundance range.Data from ProteomeXchange, PXD ID: PXD000747. Experiment: EXQ4_MaZe_SA_P4, file: folder summary. Published as part of Mol Cell Proteomics. 2014 Dec;13(12):3435-45 . From the Abstract: {{i}} ... Using recent technological advances, we here characterized the proteome of murine platelets, key actors in mediating hemostasis and thrombosis ... {{/i}}2015-08-19GPM3232001773425205226PXD000747PXD000747