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available"],"repository":["GPMDB"],"pubmed_abstract":["Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes in diverse cells and tissues at a depth of >10,000 sites. We apply this technology to generate time-resolved maps of insulin signaling in the mouse liver. Our results reveal that insulin affects ~10% of the liver phosphoproteome and that many known functional phosphorylation sites, and an even larger number of unknown sites, are modified at very early time points (<15 s after insulin delivery). Our kinetic data suggest that the flow of signaling information from the cell surface to the nucleus can occur on very rapid timescales of less than 1 min in vivo. EasyPhos facilitates high-throughput phosphoproteomics studies, which should improve our understanding of dynamic cell signaling networks and how they are regulated and dysregulated in disease."],"pubmed_title":["High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics."],"pubmed_authors":["Humphrey Sean J SJ,Azimifar S Babak SB,Mann Matthias M,","Humphrey Sean J SJ, Azimifar S Babak SB, Mann Matthias M"],"name_synonyms":["Regular Insulin, Chain, Soluble, Iletin, Insulin B Chain, biological signaling, Regular, Sodium Insulin, signalling process, signaling process, Insulin, INS, Insulin A Chain, Sodium, Novolin, Insulin B, Soluble Insulin, single organism signaling., signalling"],"description_synonyms":["Nat, NAT, Soluble, data, Insulin B Chain, scale tissue, biological signaling, Regular, D17H6S113E, wide/broad, Tissue., peltate hair, Insulin B, Spectrum Analyses, broad, FSH, Spectrum Analysis, Cell, Fsrg1, Regular Insulin, Spectroscopy, protein amino acid phosphorylation, RING3, Sodium Insulin, Insulin, AW228947, FSRG1, Insulin A Chain, Mass, Sodium, Novolin, mKIAA4005, Analysis, scales, Soluble Insulin, Mass Spectrum Analyses, Mass Spectroscopy, Mass Spectrum Analysis, Chain, Mass Spectrum, study, D6S113E, scale, Analyses, plant peltate hair, INS, RNF3, Rnf3, Frg-1, Spectrometry, Ring3, signalling, Iletin, wide, signalling process, signaling process, Fsrg-1, single organism signaling"],"pubmed_title_synonyms":["Regular Insulin, Chain, Soluble, Iletin, Insulin B Chain, biological signaling, Regular, Sodium Insulin, signalling process, signaling process, Insulin, INS, Insulin A Chain, Sodium, Novolin, Insulin B, Soluble Insulin, single organism signaling., signalling"],"pubmed_abstract_synonyms":["Soluble, 3.4.22.-, scale tissue, Insulin B Chain, biological signaling, advanced, Effects, Laboratory, peltate hair, Mus domesticus, number, mini-ICE, CASP-14, Insulin B, Spectrum Analyses, broad, House Mouse, phosphorylation, Long Term, cell associated, jecur, protein amino acid phosphorylation, period, Readability, House, Insulin, horsetail nucleus, Mass, Mus musculus domesticus, Analysis, Effect, Mice, Mass Spectroscopy, Mass Spectrum Analysis, Chain, study, neuronal nucleus, human disease, Analyses, Longterm, Swiss, cell, plant peltate hair, nucleus of CNS, Tissue, iecur, min, Swiss Mice, Long-Term, number of, \"mouse\" EXACT common_name [], \"Mus muscaris\" RELATED misnomer [], Iletin, nucleus, signaling process, has or lacks parts of type, \"mice C57BL/6xCBA/CaJ hybrid\" RELATED misspelling [], Homo sapiens disease, Long-Term Effect, house mouse, Long-Term Effects, single organism signaling, data, Regular, wide/broad, Entire liver, Arts, mouse, Longterm Effect, Phosphorylations, extra or missing physical or functional parts, Spectrum Analysis, Cell, results, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, Regular Insulin, Spectroscopy, mereological quality, Sodium Insulin, Mus, nervous system nucleus, Diseases., Long Term Effects, Mini-ICE, Insulin A Chain, precocious, cell nucleus, Sodium, Novolin, scales, Soluble Insulin, Mass Spectrum Analyses, Mass Spectrum, Mus musculus, Industrial, Industrial Arts, scale, Caspase-14 subunit p10, mice, INS, Swiss Mouse, Spectrometry, House Mice, Caspase-14 subunit p19, nucleus of neuraxis, Understanding, MICE, neuraxis nucleus, signalling, Longterm Effects, early, Laboratory Mice, domesticus, \"house mouse\" EXACT genbank_common_name [], Livers, wide, signalling process, cardinality, Mouse, cell bound, time, Laboratory Mouse"],"view_count":["25"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320017798"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.0077112893275755705"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.","description":"Data from ProteomeXchange, PXD ID: PXD001792. File: 2_QEp2_LC11_SeHu_LivPhos_Insulin_10m_Bio3.mzml. Published as part of Nat Biotechnol. 2015 Aug 17  . From the Abstract: {{i}} Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes in diverse cells and tissues ... {{/i}}","dates":{"submission":"2015-08-27"},"accession":"GPM32320017798","cross_references":{"pubmed":["26280412"],"Pride":["PXD001792"],"pride":[],"Pride Archive":["PXD001792"]}}