{"database":"GPMDB","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":11,"searchCount":5},"additional":{"omics_type":["Other"],"submitter":["Kieselbach T"],"instrument_platform":["Instrument"],"disease":["Not Available"],"brenda_tissue":["Not available"],"species":["Aggregatibacter_actinomycetemcomitans_anh9381, Aggregatibacter_actinomycetemcomitans_d11s_1"],"submitter_mail":["thomas.kieselbach@chem.umu.se"],"publication":["26381655"],"submitter_affiliation":["Department of Chemistry, Umea University"],"model":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018109","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018110","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018111","http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018112"],"cell_type":["Not available"],"repository":["GPMDB"],"pubmed_abstract":["Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we studied the proteome of density gradient-purified OMVs from a rough-colony type clinical isolate, strain 173 (serotype e) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). This analysis yielded the identification of 151 proteins, which were found in at least three out of four independent experiments. Data are available via ProteomeXchange with identifier PXD002509. Through this study, we not only confirmed the vesicle-associated release of LtxA, and the presence of proteins, which are known to act as immunoreactive antigens in the human host, but we also identified numerous additional putative virulence-related proteins in the A. actinomycetemcomitans OMV proteome. The known and putative functions of these proteins include immune evasion, drug targeting, and iron/nutrient acquisition. In summary, our findings are consistent with an OMV-associated proteome that exhibits several offensive and defensive functions, and they provide a comprehensive basis to further disclose roles of A. actinomycetemcomitans OMVs in periodontal and systemic disease."],"pubmed_title":["Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles."],"pubmed_authors":["Kieselbach Thomas T,Zijnge Vincent V,Granström Elisabeth E,Oscarsson Jan J,","Kieselbach Thomas T, Zijnge Vincent V, Granström Elisabeth E, Oscarsson Jan J"],"name_synonyms":["Actinobacillus (Haemophilus) actinomycetemcomitans, Bacterium comitans, Actinobacillus actinomycetemcomi, Actinobacillus actinomycetemcomitans, Haemophilus actinomyceticomitans, Haemophilus actinomycetemcomitans., Bacterium actinomycetem comitans, Bacterium acetinomycetum comitans"],"description_synonyms":["LC-MS2, liquid chromatography tandem mass spectroscopy, IGF-I, data, steel factor, PLXN5, PreP, Slf, hematopoietic growth factor KL, D10Wsu136e, Somatomedin-C, LC-MS/MS, Nl1, somatomedin, A4, FPH2, PLEXIN-B1, Mell1, coarse, AA410010, infectivity, TYPE, sKITLG, SeP, LGMD2C, LC-MS-MS, strain, DAGA4, MP-1, Readability, LC/MS/MS, Experiment, density gradient, CEH, MMEL2, mechano growth factor, LC-MSMS, Pop, Pathogenicity, NEPII, NL1, cultivar, NL2, SEH, MAM, Igf-1, cell separation by density gradient centrifugation, SCG3, SEP, SHEP7, LCMSMS, mast cell growth factor, DMDA1, Stem cell factor, Ntup1, somatomedin-C, 2310012C15Rik, SF, Kitl, AI047692, mKIAA1104, Understanding, STAT5, Mast cell growth factor, ecotype, sEP, stem cell factor, KL-1, DMDA, IGF1, liquid chromatography-tandem mass spectroscopy, MGF, c-Kit ligand, SCARMD2, MP1, Mechano growth factor, SELP, KITLG, liquid chromatography tandem mass spectrometry, LC-MS-MS., Eph2, virulence, PE, Soluble KIT ligand, AI450383, SCF, associated, Sl, PEP, Proteomes, NEP2"],"pubmed_title_synonyms":["Actinobacillus (Haemophilus) actinomycetemcomitans, Bacterium comitans, Actinobacillus actinomycetemcomi, Actinobacillus actinomycetemcomitans, Haemophilus actinomyceticomitans, Haemophilus actinomycetemcomitans., Bacterium actinomycetem comitans, Bacterium acetinomycetum comitans"],"pubmed_abstract_synonyms":["liquid chromatography tandem mass spectroscopy, host organism, Actinobacillus actinomycetemcomi, Cdt-I toxin, 2410041A17Rik, determination, ACTG, ACTE, nonspecific immune response, cytolethal distending toxin C, cytolethal distending toxin B, leucotoxins, A4, Homo sapiense, cytolethal distending toxin A, infectivity, Readability, unspecified, Homo sapien, ACUG, Homo sapians, Pericementitides, Antigen, symptoms, HEL-176, Endocarditides, 26Fe, CLR7.1, Nutrient, NOD2B, viral infection, human disease, Homo sapients, LCMSMS, Identification, CdtC protein, Endocarditis (disorder), InChI=1/Fe, E coli, proteins, virus process, ecotype, CD, Immune, Homo sapience, medicine, ACT, Act, Homo sapiens disease, associated, evasion or tolerance of immune response of other organism during symbiotic interaction, iron atom, Home sapiens, Infective, LC-MS2, screening, cytotoxin C (Pasteurella haemolytica), non-infective endocarditis, F830032C23Rik, InChIKey=XEEYBQQBJWHFJM-UHFFFAOYAG, CTE-II, AACT, LC-MS/MS, CTE-IIa, Evasion, hBACH, Ach1, ACH1, Actinobacillus actinomycetemcomitans, acute endocarditis (disorder), LACH1, Periodontitis (disorder), GIG25, C230079P11, Iron 56, count, Exhibits as Topics, Alpha-1-antichymotrypsin His-Pro-less, Homo sapian, Diseases., membrane-enclosed vesicle, Pathogenicity, DFNA26, NOS, concluding remarks, Card15, cultivar, immune evasion, Endocarditis, LACH, CLD-toxin, DFNA20, CdtA protein, conclusion, signs, Identifications (Psychology), Card4, Nutrients, Homo sapines, human, Bacterium acetinomycetum comitans, Endocarditis NOS, summary, RGD1562269, Eisen, DMDA, ACTL3, CARD4, Lach1, liquid chromatography-tandem mass spectroscopy, BACH, Proteomes, human being, [Fe], Periodontitides, Exhibits, number, Gene, Serpin A3, Bach, presence, TYPE, LC-MS-MS, Infective Endocarditides, DAGA4, Homo spaiens, CDT-III protein, conclusions, Immune Evasions, LC-MSMS, Pericementitis, Gene Products, Cell growth-inhibiting gene 24|25 protein, Periodontitis, MAM, CARD15, SCG3, Bacterium actinomycetem comitans, study, hierro, acute endocarditis NOS (disorder), PSORAS1, BRWS2, nutrients, man, Haemophilus actinomycetemcomitans, IBD1, drugs, 1700027G07Rik, Homo sampiens, CDT-III, Evasions, Bacterium comitans, species, membrane-bounded vesicle, Cte-II, nutrient, data, findings, CdtB protein, drug, Proteins, Actinobacillus (Haemophilus) actinomycetemcomitans, dJ393D12.2, fer, innate immunity, coarse, Cell, LGMD2C, strain, polypeptide, count in organism, LC/MS/MS, density gradient, Protein, chemical analysis, Homo sapeins, ACTA3, cell separation by density gradient centrifugation, Foods, CLR16.3, ferrum, F830007N14Rik, acute endocarditis, cytolethal distending toxin, DMDA1, NLRC1, NLRC2, VSCM, LTX, Humo sapiens, Understanding, Iron-56, Protein Gene Products, BLAU, Gene Proteins, Homo spiens, \"human\" EXACT genbank_common_name [], acute Endocarditis, SCARMD2, Infective Endocarditis, liquid chromatography tandem mass spectrometry, virulence, ACTSG, assay, quantitative, Haemophilus actinomyceticomitans, Nlrc2, Nlrc1, presence or absence in organism"],"view_count":["11"],"citation_count":["0"],"search_count":["5"],"full_dataset_link":["http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018111"],"search_domains":["dbgap_ncbi~0","patentfamilies~0","rfam~0","merops~0","complex-portal~0","uniprot~0","wormbaseparasite~0","embl-covid19~0","reactome~0","emdb~0","wgs_masters~0","ebiweb_resources~0","opentargets_genetics~0","biomodels_all~0","ipd-mhc~0","ebiweb_teams~0","taxonomy~0","genome_assembly~0","sc-experiments~0","ebiweb_people~0","enzymeportal_enzymes~0","ipd-nhkir~0","cellosaurus~0","pdbe~0","chebi~0","patentproteins~0","interpro7~0","uniref~0","chembl~0","pdbekb~0","gpcrdb~0","hgnc~0","sc-genes~0","intact~0","rhea~0","ebiweb_training~0","alphafold~0","imgt-hla~0","patentnucleotides~0","ensemblroot~0","eva_studies~0","non-coding~0","europepmc~0","pubmed~1","identifiers_registry~0","pdbechem~0","hpa-covid19~0","eva-variants-covid19~0","biosamples~0","gwas_catalog~0","biotools~0","tls_masters~0","mesh~0","coding~0","sra~0","opentargets~0","efo~0","embl-pathogen~0","project~0","pride~1","human_diseases~0","geo_datasets~0","embl~0","treefam~0","uniparc~0","ols~0","dgva~0","intenz~0","go~0","tsa_masters~0","biosamples-covid19~0","ebiweb_corporate~0","omim~0","lrg~0","earlycause-molecular-sequences~0","ipd-kir~0","empiar~0","rnacentral~0","orcid_data_claims~0","gpmdb~2","lineage-covid19~0","metagenomics~0","pfam~0","pride archive~1","varsite~0"],"reanalysis_count":["0"],"submitter_keywords":["Resource Reanalysis"],"citation_count_scaled":["0.0"],"reanalysis_count_scaled":["0.0"],"view_count_scaled":["0.003392967304133251"],"download_count_scaled":["0.0"],"normalized_connections":["1.0"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles.","description":"Data from ProteomeXchange, PXD ID: PXD002509. File: 2013_10_16_Prep_3_Serotype_e_third_experiment_combined_mgf.mgf. Published as part of PLoS One. 2015 Sep 18;10(9):e0138591  . From the Abstract: {{i}} ... To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we studied the proteome of density gradient-purified OMVs from a rough-colony type clinical isolate, strain 173 (serotype e) using liquid chromatography-tandem mass spectrometry (LC-MS/MS) ... {{/i}}","dates":{"submission":"2015-09-21"},"accession":"GPM32320018111","cross_references":{"pubmed":["26381655"],"Pride":["PXD002509"],"pride":[],"Pride Archive":["PXD002509"]}}