GPMDB00655OtherShuai Li, et al.InstrumentNot AvailableNot availableMus_musculus_viruses, Mouseshuai.li@med.uni-muenchen.de26408665http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018780http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018781http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018814http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018815http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018812http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018779http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018813http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018818http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018819http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018816http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018817http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018773http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018774http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018777http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018810http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018811http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018778http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018776http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018791http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018792http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018790http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018825http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018826http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018823http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018824http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018829http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018827http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018828http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018785http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018788http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018821http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018822http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018789http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018786http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018820http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018787http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018795http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018796http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018793http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018794http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018799http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018832http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018830http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018797http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018798http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018831http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018809http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018803http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018804http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018801http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018802http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018807http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018808http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018805http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018806http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018800Institute for Immunology, Ludwig-Maximilians-University, et al.Not availableGPMDBTissues accommodate defined numbers of dendritic cells (DCs) in highly specific niches where different intrinsic and environmental stimuli control DC life span and numbers. DC homeostasis in tissues is important, because experimental changes in DC numbers influence immunity and tolerance toward various immune catastrophes and inflammation. However, the precise molecular mechanisms regulating DC life span and homeostasis are unclear. We report that the GTPase RhoA controls homeostatic proliferation, cytokinesis, survival, and turnover of cDCs. Deletion of RhoA strongly decreased the numbers of CD11b(-)CD8(+) and CD11b(+)Esam(hi) DC subsets, whereas CD11b(+)Esam(lo) DCs were not affected in conditional RhoA-deficient mice. Proteome analyses revealed a defective prosurvival pathway via PI3K/protein kinase B (Akt1)/Bcl-2-associated death promoter in the absence of RhoA. Taken together, our findings identify RhoA as a central regulator of DC homeostasis, and its deletion decreases DC numbers below critical thresholds for immune protection and homeostasis, causing aberrant compensatory DC proliferation.Control of Homeostasis and Dendritic Cell Survival by the GTPase RhoA.Li Shuai S,Dislich Bastian B,Brakebusch Cord H CH,Lichtenthaler Stefan F SF,Brocker Thomas T,Li Shuai S, Dislich Bastian B, Brakebusch Cord H CH, Lichtenthaler Stefan F SF, Brocker Thomas TAutoregulation, Cell Viabilities, Arha2, ARH12, Controlling, activation of homeostatic process, reference sample, positive regulation of homeostatic process, Survival, Arha1, negative regulation of homeostatic process, homeostasis, ARHA, Arha, RHOH12, inhibition of homeostatic process, regulation of homeostatic process, Viabilities, Cell Viability, RHO12., Cell, Viability, ControlledAutoregulation, Interstitial Dendritic Cells, Dendritic Cells, data, Veiled Cells, Controlling, PLXN5, Inflammations, experimental, Mthfd, Interdigitating, Nl1, PLEXIN-B1, Mell1, Arha, Interstitial, Cell, Divisions, SeP, RHO12, Cytokineses, Arha2, ARH12, Plasmacytoid Dendritic Cells, CEH, survival, positive regulation of homeostatic process, MMEL2, Cytoplasmic Division, Arha1, GRIM-12, NEPII, NL1, Interdigitating Dendritic Cell, NL2, Dcs, SEH, Dendritic Cell, regulation of homeostatic process, Interstitial Dendritic Cell, SEP, Plasmacytoid Dendritic Cell, methods, cell cycle cytokinesis, inflammatory response, reference sample, DC homeostasis, death rate, Plasmacytoid Dendritic, Veiled Cell, E430024A07Rik, experimental section, Interdigitating Dendritic, Dendritic, Veiled, Interdigitating Cells, Tissue, Plasmacytoid, inflammation, inhibition of homeostatic process, Division, sEP, experimental procedures, Cytoplasmic, Interstitial Dendritic, activation of homeostatic process, cytokinesis involved in cell cycle, Inflammation, TrxR1, time of survival., Cells, TXNR, SELP, negative regulation of homeostatic process, Interdigitating Dendritic Cells, homeostasis, TR1, Cytoplasmic Divisions, Interdigitating Cell, Eph2, ARHA, RHOH12, NEP2, TR, Controlled, TRXR1Autoregulation, Cell Viabilities, Arha2, ARH12, Controlling, activation of homeostatic process, reference sample, positive regulation of homeostatic process, Survival, Arha1, negative regulation of homeostatic process, homeostasis, ARHA, Arha, RHOH12, inhibition of homeostatic process, regulation of homeostatic process, Viabilities, Cell Viability, RHO12., Cell, Viability, ControlledDendritic Cells, deceased, PRKBA, Inflammations, Laboratory, Interdigitating, Mus domesticus, PI3K, cell surface glycoprotein MAC-1 subunit alpha, CASP-14, Cardiac Death, AKT1, PPP1R50, House Mouse, Divisions, RHO12, Cytokineses, p110-alpha, symptoms, Interdigitating Dendritic Cell, phosphatidylinositol 3-kinase activity, Dendritic Cell, Interstitial Dendritic Cell, present in fewer numbers in organism, inflammatory response, reference sample, catalyst activity, Tissue, Plasmacytoid, Swiss Mice, PI3-kinase activity, inhibition of homeostatic process, PI3Kgamma, PKB|Akt, MCAP, cytokinesis involved in cell cycle, decreased, p55alpha, Inflammation, homeostasis, associated, MCMTC, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, CR-3 alpha chain, PKB alpha, Interstitial Dendritic Cells, screening, Protein kinase B, aberrant, functional failure, P110DELTA, PtdIns-3-kinase activity, mouse, p50alpha, RAC-PK-alpha, Interstitial, 2310008D05Rik, 1-phosphatidylinositol 3-kinase activity, AKT1 kinase, Plasmacytoid Dendritic Cells, positive regulation of homeostatic process, Mini-ICE, Proto-oncogene c-Akt, Near-Death Experience, concluding remarks, failure, regulator, Bcl-2, Mus musculus, cell cycle cytokinesis, absence, death rate, Caspase-14 subunit p10, E430024A07Rik, p85alpha, Determination of Death, mice, p32, Veiled, conclusion, Swiss Mouse, signs, Caspase-14 subunit p19, proto-oncogene c-Akt, MICE, experimental procedures, domesticus, protein kinase B, APDS, summary, p110D, W117m, PI3KBETA, Cells, leukocyte adhesion receptor MO1, Interdigitating Dendritic Cells, ARHA, Mouse, SLEB6, p120-PI3K, RHOH12, Proteomes, RAC-ALPHA, CR3A, 3.4.22.-, atypia, experimental, Arha, mini-ICE, disfunctional, type I phosphatidylinositol kinase activity, phosphatidylinositol 3-kinase, ARH12, Leukocyte adhesion receptor MO1, PIK3, Cardiac, conclusions, reduced, House, subnumerary, Mus musculus domesticus, atypical, aberrant., MAL, Mice, class I, methods, MAC-1, Plasmacytoid Dendritic, Swiss, experimental section, Protein kinase B alpha, Interdigitating Dendritic, Dendritic, neutrophil adherence receptor, absent from organism, inflammation, decreased number, Division, Cytoplasmic, P110BETA, Interstitial Dendritic, CD11 antigen-like family member B, AKT, time of survival, Akt, AW986256, negative regulation of homeostatic process, MO1A, MCM, Controlled, Autoregulation, ITGAM, Veiled Cells, Controlling, findings, Mthfd, class II, CD11b, type III phosphoinositide 3-kinase activity, Neutrophil adherence receptor, Leu2, Vps34p, defective, Cell, Arha2, PIK3C1, 2.7.11.1, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, PKB, survival, CWS6, CWS5, Mus, Cytoplasmic Division, Arha1, PKB-ALPHA, post-mortem, Dcs, PI3CG, regulation of homeostatic process, Death, MAC1A, death, Plasmacytoid Dendritic Cell, DC homeostasis, Veiled Cell, Interdigitating Cells, class III, House Mice, C430015F12Rik, p110gamma, Laboratory Mice, activation of homeostatic process, CLOVE, D830018M01Rik, DEL, IMD14, Cell surface glycoprotein MAC-1 subunit alpha, PKBalpha, CD11B, Cytoplasmic Divisions, Interdigitating Cell, D630044D05Rik, Esam1, RAC, Rac, CD8, Laboratory Mouse, Thymoma viral proto-oncogene, thymoma viral proto-oncogene6505http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320018773dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0200493522516964830.01.0falseControl of Homeostasis and Dendritic Cell Survival by the GTPase RhoAData from ProteomeXchange, PXD ID: PXD001636. File: C1_1_TR1.mzml. Published as part of J Immunol. 2015 Sep 25 . From the Abstract: {{i}} Tissues accommodate defined numbers of dendritic cells (DCs) in highly specific niches where different intrinsic and environmental stimuli control DC life span and numbers. DC homeostasis in tissues is important, because experimental changes in DC numbers influence immunity and tolerance toward various immune catastrophes and inflammation. However, the precise molecular mechanisms regulating DC life span and homeostasis are unclear. We report that the GTPase RhoA controls homeostatic proliferation, cytokinesis, survival, and turnover of cDCs ... {{/i}}2015-10-02GPM3232001877326408665PXD001636PXD001636