GPMDB00275OtherSury MD, et al.InstrumentNot AvailableNot availableDmelanogastermatthias.selbach@mdc-berlin.de20525996http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019430http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019429http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019428http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019423http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019422http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019421http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019420http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019427http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019426http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019425http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019424http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019441http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019440http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019439http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019434http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019433http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019399http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019432http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019431http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019398http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019438http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019437http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019436http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019435http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019452http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019451http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019450http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019445http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019444http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019443http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019442http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019449http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019448http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019447http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019446http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019463http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019462http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019461http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019460http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019456http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019455http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019454http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019453http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019459http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019458http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019457http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019470http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019474http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019473http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019472http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019471http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019508http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019507http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019506http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019505http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019509http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019467http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019500http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019466http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019465http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019464http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019504http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019503http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019469http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019502http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019468http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019501http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019481http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019480http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019485http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019484http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019483http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019482http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019517http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019516http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019478http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019511http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019477http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019510http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019476http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019475http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019515http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019514http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019513http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019512http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019479http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019492http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019491http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019490http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019496http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019495http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019494http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019493http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019409http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019408http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019407http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019406http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019489http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019401http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019400http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019488http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019487http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019486http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019405http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019404http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019403http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019402http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019419http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019417http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019412http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019411http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019499http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019410http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019498http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019497http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019416http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019415http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019414http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019413Max Delbr-ck Center for Molecular MedicineNot availableGPMDBStable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the most widely used small animal models in biology. Here, we show that feeding flies with SILAC-labeled yeast leads to almost complete labeling in the first filial generation. We used these "SILAC flies" to investigate sexual dimorphism of protein abundance in D. melanogaster. Quantitative proteome comparison of adult male and female flies revealed distinct biological processes specific for each sex. Using a tudor mutant that is defective for germ cell generation allowed us to differentiate between sex-specific protein expression in the germ line and somatic tissue. We identified many proteins with known sex-specific expression bias. In addition, several new proteins with a potential role in sexual dimorphism were identified. Collectively, our data show that the SILAC fly can be used to accurately quantify protein abundance in vivo. The approach is simple, fast, and cost-effective, making SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.The SILAC fly allows for accurate protein quantification in vivo.Sury Matthias D MD,Chen Jia-Xuan JX,Selbach Matthias M,Sury Matthias D MD, Chen Jia-Xuan JX, Selbach Matthias Mpolypeptide, proteins.OCT, 3.4.22.-, fruit fly, multi-cellular organism, mol, PLXN2, Laboratory, Aminosaeure, Mus domesticus, Aminocarbonsaeure, baker's yeast, mini-ICE, CASP-14, Sophophora melanogaster, Isotope-Coded Affinity, melanogaster, House Mouse, Saccharomyces italicus, Tier, reduced, House, Drosophila melangaster, yeast, Mus musculus domesticus, tiny, Isotopically-Coded Affinity, animal, Mice, Isotopically-Coded Affinity Tagging, lager beer yeast, amino acids, cell, Swiss, labeling., hypoplasia, Saccharomyes cerevisiae, 2810428A13Rik, Swiss Mice, proteins, metazoa, "mouse" EXACT common_name [], Saccharomyces uvarum var. melibiosus, "Mus muscaris" RELATED misnomer [], Candida robusta, Stable, Saccharomyces capensis, Isotope-Coded Affinity Tagging, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], house mouse, brewer's yeast, Tagging, mKIAA0463, Stable Isotope Labeling, small, flies, data, PlexA2, body, Aminokarbonsaeure, animalia, mouse, whole body, AA589422, Labeling, D. melanogaster, Cell, Saccaromyces cerevisiae, mice C57BL/6xCBA/CaJ hybrid, Mus muscaris, polypeptide, Sccharomyces cerevisiae, Isotope, organism, AW457381, Mus, Stable Isotope, Mini-ICE, Isotope Coded Affinity Tagging, Saccharomyces oviformis, Mus musculus, Yeast, whole organism, Caspase-14 subunit p10, underdeveloped, mice, Swiss Mouse, House Mice, Caspase-14 subunit p19, Isotope Labeling, Amino, MICE, Affinity Tagging, Isotopically-Coded, Laboratory Mice, domesticus, "house mouse" EXACT genbank_common_name [], Drosophila, Drosophila melanogasters, Koerper, Isotope-Coded, Acids, Mouse, Plxn2, Laboratory Mousepolypeptide, proteins.projections, Scientific Bias, fruit fly, Laboratory, adult stage, Aminosaeure, Mus domesticus, CASP-14, melanogaster, House Mouse, Tier, Roles, Epidemiologic Biase, Concepts, Ecological, animal, adult, Isotopically-Coded Affinity Tagging, male, portion of tissue, Ecological Biases, amino acids, Sex, developmental field, Tissue, hypoplasia, male genitalia, Saccharomyes cerevisiae, Swiss Mice, proteins, Saccharomyces uvarum var. melibiosus, Fast, Phenotypic, Saccharomyces capensis, Statistical Biases, papilla, Role Concepts, Ecological Bias, "mice C57BL/6xCBA/CaJ hybrid" RELATED misspelling [], Systematic, house mouse, brewer's yeast, Tagging, Epidemiologic Biases, SIMPLE, Stable Isotope Labeling, FAST, male genital system, anatomical protrusion, Scientific, http://mged.sourceforge.net/ontologies/MGEDOntology.owl#sex, aberrant, functional failure, Aminokarbonsaeure, animalia, Epidemiologic, mouse, lamina, flanges, FASTK, future organ, Ecological Fallacies, female, mice C57BL/6xCBA/CaJ hybrid, Outcome Measurement Errors, organism, Experimental, Role Concept, count, PIG7, Mini-ICE, gamete, shelf, Role, tissue portion, simple tissue, failure, Truncation Bias, Adults, finances, Mus musculus, Genotypic, Yeast, Bias, adults, http://mged.sourceforge.net/ontologies/MGEDOntology.owl#female, whole organism, Caspase-14 subunit p10, mice, shelves, Swiss Mouse, salaries, Caspase-14 subunit p19, Outcome Measurement, MICE, projection, ridge, domesticus, Experimental Bias, organ system, sex-specific, Outcome Measurement Error, Drosophila, spine, Koerper, Acids, Mouse, financial management, Proteomes, 3.4.22.-, atypia, multi-cellular organism, lamellae, Ecological Fallacy, Truncation, Aminocarbonsaeure, number, STK10, baker's yeast, Gene, Systematic Bias, mini-ICE, Sophophora melanogaster, disfunctional, http://mged.sourceforge.net/ontologies/MGEDOntology.owl#male, Isotope-Coded Affinity, presence, process of organ, body system, Saccharomyces italicus, protrusion, lamella, organ field, reduced, House, Drosophila melangaster, yeast, Gene Products, Mus musculus domesticus, system, field, atypical, tiny, Isotopically-Coded Affinity, Mice, lager beer yeast, Fallacies, anatomical systems, Genotypic Sex, Swiss, Aggregation, metazoa, labeling, ridges, financing, "mouse" EXACT common_name [], "Mus muscaris" RELATED misnomer [], funding, Candida robusta, Stable, ATP:Fas-activated serine/threonine protein phosphotransferase activity, Isotope-Coded Affinity Tagging, laminae, Biase, TP53I7, small, flies, Fallacy, fees, data, female human body, body, Males, anatomical process, Proteins, whole body, Truncation Biases, defective, Labeling, D. melanogaster, Cell, Saccaromyces cerevisiae, Mus muscaris, Concept, polypeptide, Sccharomyces cerevisiae, Isotope, count in organism, Mus, Stable Isotope, Protein, Errors, biological sex, connected anatomical system, Isotope Coded Affinity Tagging, flange, organ process, Saccharomyces oviformis, distinct, underdeveloped, Biases, House Mice, Isotope Labeling, Amino, Affinity Tagging, Isotopically-Coded, Laboratory Mice, male human body, Protein Gene Products, "house mouse" EXACT genbank_common_name [], process, processes, Gene Proteins, cost, presence or absence in organism., gender-specific anatomical structure, Error, Aggregation Bias, Phenotypic Sex, Drosophila melanogasters, Statistical Bias, Isotope-Coded, gender-specific, processus, quantitative, Statistical, Laboratory Mouse, Females, presence or absence in organism2705http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019419dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0083281924737816160.01.0falseThe SILAC fly allows for accurate protein quantification in vivo.Data from ProteomeXchange, PXD ID: PXD003040. File: Bert_20090626_JC_tud_M_standard_slice_11_2.mzml. Published as part of Mol Cell Proteomics. 2010 Oct;9(10):2173-83 . From the Abstract: {{i}} Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the most widely used small animal models in biology. Here, we show that feeding flies with SILAC-labeled yeast leads to almost complete labeling in the first filial generation. {{/i}}2015-10-13GPM3232001941920525996PXD003040PXD003040