GPMDB00375OtherWendler S, et al.InstrumentNot AvailableNot availableActinoplanes_sp_se50_110wendler@cebitec.uni-bielefeld.de25896738http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019706http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019705http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019826http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019704http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019825http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019703http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019824http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019709http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019829http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019708http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019707http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019828http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019702http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019823http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019701http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019822http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019700http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019821http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019820http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019838http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019717http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019716http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019837http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019836http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019715http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019714http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019835http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019719http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019718http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019839http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019830http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019713http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019834http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019833http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019712http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019711http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019832http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019710http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019831http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019728http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019849http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019848http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019727http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019726http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019847http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019725http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019846http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019729http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019841http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019720http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019840http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019724http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019845http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019723http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019844http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019722http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019843http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019842http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019721http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019739http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019738http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019859http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019737http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019858http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019857http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019736http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019731http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019852http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019730http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019851http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019850http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019856http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019735http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019734http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019855http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019854http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019733http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019853http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019732http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019742http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019862http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019741http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019740http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019861http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019860http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019745http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019744http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019743http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019760http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019759http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019758http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019757http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019756http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019755http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019771http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019770http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019769http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019764http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019763http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019762http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019761http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019768http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019767http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019766http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019765http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019661http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019782http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019660http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019781http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019780http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019659http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019775http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019774http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019773http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019772http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019779http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019658http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019778http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019657http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019777http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019656http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019776http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019793http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019672http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019671http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019792http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019670http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019791http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019790http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019786http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019665http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019664http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019785http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019784http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019663http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019783http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019662http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019669http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019789http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019668http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019788http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019667http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019787http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019666http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019683http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019682http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019681http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019680http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019797http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019676http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019796http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019675http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019674http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019795http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019794http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019673http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019679http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019678http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019799http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019677http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019798http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019690http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019694http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019693http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019692http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019691http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019687http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019686http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019685http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019684http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019689http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019688http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019698http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019697http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019696http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019695http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019699http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019805http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019804http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019803http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019802http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019809http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019808http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019807http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019806http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019801http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019800http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019816http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019815http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019814http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019813http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019819http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019818http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019817http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019812http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019811http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019810Senior Research Group in Genome Research of Industrial Microorganisms, Center for Biotechnology, Bielefeld University, et al.Not availableGPMDBActinoplanes sp. SE50/110 is a natural producer of the α-glucosidase inhibitor acarbose, a bacterial secondary metabolite that is used as a drug for the treatment of type 2 diabetes, a disease which is a global pandemic that currently affects 387 million people and accounts for 11% of worldwide healthcare expenditures (www.idf.org). The work presented here is the first comprehensive investigation of protein localization and abundance in Actinoplanes sp. SE50/110 and provides an extensive source of information for the selection of genes for future mutational analysis and other hypothesis driven experiments. The conclusion that acarbose or pyochelin family siderophores are synthesized at the inner side of the cytoplasmic membrane determined from this work, indicates that studying corresponding intermediates will be challenging. In addition to previous studies on the genome and transcriptome, the work presented here demonstrates that the next omic level, the proteome, is now accessible for detailed physiological analysis of Actinoplanes sp. SE50/110, as well as mutants derived from this and related species.Acarbose is an α-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and spectral counting to assess the relative abundance of proteins within fractions was applied. The analysis of four different proteome fractions (cytosolic, enriched membrane, membrane shaving and extracellular fraction) resulted in the identification of 1582 of the 8270 predicted proteins. All 22 Acb-proteins and 21 of the 23 Pch-proteins were detected. Predicted membrane-associated, integral membrane or extracellular proteins of the pch and the acb gene cluster were found among the most abundant proteins in corresponding fractions. Intracellular biosynthetic proteins of both gene clusters were not only detected in the cytosolic, but also in the enriched membrane fraction, indicating that the biosynthesis of acarbose and putative pyochelin metabolites takes place at the inner membrane.Acarbose is an α-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and spectral counting to assess the relative abundance of proteins within fractions was applied. The analysis of four different proteome fractions (cytosolic, enriched membrane, membrane shaving and extracellular fraction) resulted in the identification of 1582 of the 8270 predicted proteins. All 22 Acb-proteins and 21 of the 23 Pch-proteins were detected. Predicted membrane-associated, integral membrane or extracellular proteins of the pch and the acb gene cluster were found among the most abundant proteins in corresponding fractions. Intracellular biosynthetic proteins of both gene clusters were not only detected in the cytosolic, but also in the enriched membrane fraction, indicating that the biosynthesis of acarbose and putative pyochelin metabolites takes place at the inner membrane.Actinoplanes sp. SE50/110 is a natural producer of the α-glucosidase inhibitor acarbose, a bacterial secondary metabolite that is used as a drug for the treatment of type 2 diabetes, a disease which is a global pandemic that currently affects 387 million people and accounts for 11% of worldwide healthcare expenditures (www.idf.org). The work presented here is the first comprehensive investigation of protein localization and abundance in Actinoplanes sp. SE50/110 and provides an extensive source of information for the selection of genes for future mutational analysis and other hypothesis driven experiments. The conclusion that acarbose or pyochelin family siderophores are synthesized at the inner side of the cytoplasmic membrane determined from this work, indicates that studying corresponding intermediates will be challenging. In addition to previous studies on the genome and transcriptome, the work presented here demonstrates that the next omic level, the proteome, is now accessible for detailed physiological analysis of Actinoplanes sp. SE50/110, as well as mutants derived from this and related species.<h4>Unlabelled</h4>Acarbose is an α-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and spectral counting to assess the relative abundance of proteins within fractions was applied. The analysis of four different proteome fractions (cytosolic, enriched membrane, membrane shaving and extracellular fraction) resulted in the identification of 1582 of the 8270 predicted proteins. All 22 Acb-proteins and 21 of the 23 Pch-proteins were detected. Predicted membrane-associated, integral membrane or extracellular proteins of the pch and the acb gene cluster were found among the most abundant proteins in corresponding fractions. Intracellular biosynthetic proteins of both gene clusters were not only detected in the cytosolic, but also in the enriched membrane fraction, indicating that the biosynthesis of acarbose and putative pyochelin metabolites takes place at the inner membrane.<h4>Biological significance</h4>Actinoplanes sp. SE50/110 is a natural producer of the α-glucosidase inhibitor acarbose, a bacterial secondary metabolite that is used as a drug for the treatment of type 2 diabetes, a disease which is a global pandemic that currently affects 387 million people and accounts for 11% of worldwide healthcare expenditures (www.idf.org). The work presented here is the first comprehensive investigation of protein localization and abundance in Actinoplanes sp. SE50/110 and provides an extensive source of information for the selection of genes for future mutational analysis and other hypothesis driven experiments. The conclusion that acarbose or pyochelin family siderophores are synthesized at the inner side of the cytoplasmic membrane determined from this work, indicates that studying corresponding intermediates will be challenging. In addition to previous studies on the genome and transcriptome, the work presented here demonstrates that the next omic level, the proteome, is now accessible for detailed physiological analysis of Actinoplanes sp. SE50/110, as well as mutants derived from this and related species.Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster.Wendler Sergej S,Otto Andreas A,Ortseifen Vera V,Bonn Florian F,Neshat Armin A,Schneiker-Bekel Susanne S,Walter Frederik F,Wolf Timo T,Zemke Till T,Wehmeier Udo F UF,Hecker Michael M,Kalinowski Jörn J,Becher Dörte D,Pühler Alfred A,Wendler Sergej S, Otto Andreas A, Ortseifen Vera V, Bonn Florian F, Neshat Armin A, Schneiker-Bekel Susanne S, Walter Frederik F, Wolf Timo T, Zemke Till T, Wehmeier Udo F UF, Hecker Michael M, Kalinowski Jörn J, Becher Dörte D, Pühler Alfred Apyochelin biosynthetic process, determination, acarbosum, pyochelin formation, 6S)-4, 6-trihydroxy-3-(hydroxymethyl)-2-cyclohexen-1-yl)amino)-alpha-D-glucopyranosyl-(1-4)-O-alpha-D-glucopyr anosyl-(1-4)-, Proteins, Gene, pyochelin synthesis, 4 alpha, 5 beta, 4S, polypeptide, Glucor, 6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucose, D-glucose, Bay g 5421, chemical analysis, Protein, Gene Products, Actinoplanes sp. 50/110, 4, relational spatial quality, 5, O-4, placement, 5S, C25H43NO18, 6 alpha))-4, proteins, Protein Gene Products, Glumida, Gene Proteins, Acarbose, pyochelin biosynthesis, peptide modification, acarbose, Actinoplanes sp. (strain 50/110), pyochelin anabolism, 6-dideoxy-4-(((1S-(1 alpha, peptide formation., assay, Precose, Prandase, location, Proteomes, acarbosa, 6-dideoxy-4-{[(1S, GlucobayModb1, Gene., membrane, data, bioformation, DM, determination, acarbosum, 6S)-4, 6-trihydroxy-3-(hydroxymethyl)-2-cyclohexen-1-yl)amino)-alpha-D-glucopyranosyl-(1-4)-O-alpha-D-glucopyr anosyl-(1-4)-, Arts, Proteins, Gene, biosynthesis, 9930121L06Rik, membranous organ component, 4 alpha, 5 beta, exo, predicted, 4S, OB-RGRP, polypeptide, mXrn1, Experiment, Glucor, 6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucose, Dhm2, D-glucose, Glucosidase, Bay g 5421, chemical analysis, Protein, Diabetes mellitus (disorder), Gene Products, Actinoplanes sp. 50/110, NOS, 4, PCH, 5, relational spatial quality, O-4, Leprb, placement, 5S, DM - Diabetes mellitus, multicellular organismal biosynthetic process, treatment, AI661365, LEPROT, Art, single-organism biosynthetic process, membrane of organ, diabetes mellitus, Identification, formation, C25H43NO18, anabolism, 6 alpha))-4, obese-like, Identifications (Psychology), proteins, W, Obr, Diabetes NOS, Glumida, Protein Gene Products, synthesis, Acarbose, Gene Proteins, extracellular, Diabetes mellitus, acarbose, Snm1l, Pontoneocerebllar hypoplasia, Actinoplanes sp. (strain 50/110), Pontoneocerebellar atrophy, disease management, 6-dideoxy-4-(((1S-(1 alpha, assay, obl, Precose, Prandase, location, acarbosa, 6-dideoxy-4-{[(1S, Diabetes, Proteomes, Glucobay, diabetes, dbpyochelin biosynthetic process, determination, acarbosum, pyochelin formation, 6S)-4, 6-trihydroxy-3-(hydroxymethyl)-2-cyclohexen-1-yl)amino)-alpha-D-glucopyranosyl-(1-4)-O-alpha-D-glucopyr anosyl-(1-4)-, Proteins, Gene, pyochelin synthesis, 4 alpha, 5 beta, 4S, polypeptide, Glucor, 6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucose, D-glucose, Bay g 5421, chemical analysis, Protein, Gene Products, Actinoplanes sp. 50/110, 4, relational spatial quality, 5, O-4, placement, 5S, C25H43NO18, 6 alpha))-4, proteins, Protein Gene Products, Glumida, Gene Proteins, Acarbose, pyochelin biosynthesis, peptide modification, acarbose, Actinoplanes sp. (strain 50/110), pyochelin anabolism, 6-dideoxy-4-(((1S-(1 alpha, peptide formation., assay, Precose, Prandase, location, Proteomes, acarbosa, 6-dideoxy-4-{[(1S, GlucobayT2DM - Type 2 Diabetes mellitus, Extended Families, Networks, Adult-Onset Diabetes Mellitus, Materials, Family Member, Extended, Kinship, determination, AI461847, Transcriptome Profile, Noninsulin Dependent, selection process, Gene Expression Profile, Gene, Type 2 Diabetes Mellitus, Extended Family, Network, Profiles, DIABETES MELLITUS TYPE 02, diabetes mellitus type 2, Glucor, D-glucose, Non-Insulin-Dependent, Actinoplanes sp. 50/110, Non-Insulin Dependent Diabetes Mellitus, Life Cycle, Pgi, 4, non-insulin-dependent diabetes mellitus, 5, O-4, Gpi-1, Slow Onset, Family Life Cycle, treatment, study, human disease, asymmetric protein localization, Genetic, Kinship Network, Genomes, Research, Gpi-1r, Nlk, Gpi-1s, Profile, 6 alpha))-4, Phi, Gpi-1t, Maturity Onset Diabetes Mellitus, W, Signatures, NIDDM, Ketosis-Resistant Diabetes Mellitus, inner endospore membrane, plasma membrane lipid bilayer, drugs, Stable, medicine, Expression Signature, plasmalemma, Diabetes Mellitus, Actinoplanes sp. (strain 50/110), disease management, Transcriptomes, Ketosis-Resistant, Homo sapiens disease, Kinship Networks, Family Life Cycles, establishment and maintenance of asymmetric protein localization, Prandase, Non Insulin Dependent, Family, protein localisation, 6-dideoxy-4-{[(1S, Diabetes, Siderochromes, Adult Onset, Gpi, Transcriptome, Family Research, acarbosum, 6S)-4, 6-trihydroxy-3-(hydroxymethyl)-2-cyclohexen-1-yl)amino)-alpha-D-glucopyranosyl-(1-4)-O-alpha-D-glucopyr anosyl-(1-4)-, drug, Expression Profiles, MODY, Reconstituted Families, MF, 4 alpha, 5 beta, Amf, Cistrons, 4S, Adult-Onset, Family Members, whole transcriptome, Gene Expression, cellular membrane, juxtamembrane, 6-trihydroxy-3-(hydroxymethyl)cyclohex-2-en-1-yl]amino}-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-D-glucose, mOC-X, Glucosidase, Adult-Onset Diabetes, Stepfamily, Bay g 5421, species., Expression Signatures, chemical analysis, Gene Expression Signatures, Diseases, Maturity-Onset, Slow-Onset Diabetes Mellitus, Genetic Materials, Type 2 Diabetes Mellitus Non-Insulin Dependent, Ketosis Resistant, NK|GPI, bacterial inner membrane, Gene Expression Signature, Filiation, Expression Profile, Maturity-Onset Diabetes Mellitus, Genetic Material, NK, 5S, Transcriptome Profiles, Org, ORG, Type 2 Diabetes, cell membrane, Pandemic, Reconstituted Family, C25H43NO18, Type II Diabetes, Gpi1-r, Gpi1-s, whole genome, Type 2, Gpi1-t, Glumida, Acarbose, Life Cycles, Reconstituted, acarbose, Non-Insulin Dependent Diabetes, Material, Families, Gene Expression Profiles, 6-dideoxy-4-(((1S-(1 alpha, Cistron, asymmetric protein localisation, assay, establishment and maintenance of protein localization, cytoplasmic membrane, Signature, Type II, Precose, Slow-Onset, Relatives, acarbosa, Stable Diabetes Mellitus, Proteomes, Glucobay, Maturity Onset, Bglap-rs1, Stepfamilies, hypothesis, Gpi1s3705http://gpmdb.thegpm.org/~/dblist_gpmnum/gpmnum=GPM32320019694dbgap_ncbi~0patentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0embl-covid19~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0opentargets_genetics~0biomodels_all~0ipd-mhc~0ebiweb_teams~0taxonomy~0genome_assembly~0sc-experiments~0ebiweb_people~0enzymeportal_enzymes~0ipd-nhkir~0cellosaurus~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0pdbekb~0gpcrdb~0hgnc~0sc-genes~0intact~0rhea~0ebiweb_training~0alphafold~0imgt-hla~0patentnucleotides~0ensemblroot~0eva_studies~0non-coding~0europepmc~0pubmed~1identifiers_registry~0pdbechem~0hpa-covid19~0eva-variants-covid19~0biosamples~0gwas_catalog~0biotools~0tls_masters~0mesh~0coding~0sra~0opentargets~0efo~0embl-pathogen~0project~0pride~1human_diseases~0geo_datasets~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0biosamples-covid19~0ebiweb_corporate~0omim~0lrg~0earlycause-molecular-sequences~0ipd-kir~0empiar~0rnacentral~0orcid_data_claims~0gpmdb~2lineage-covid19~0metagenomics~0pfam~0pride archive~1varsite~00Resource Reanalysis0.00.00.0114127082048118450.01.0falseComprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster.Data from ProteomeXchange, PXD ID: PXD001497. Experiment: SW_Exo_G2, file: 130519_o3_p4_ao_SW_Exo_G2_8.mzml. Published as part of J Proteomics. 2015 Jul 1;125:1-16 . From the Abstract: {{i}} Acarbose is an &#945;-glucosidase inhibitor produced by Actinoplanes sp. SE50/110 that is medically important due to its application in the treatment of type2 diabetes. In this work, a comprehensive proteome analysis of Actinoplanes sp. SE50/110 was carried out to determine the location of proteins of the acarbose (acb) and the putative pyochelin (pch) biosynthesis gene cluster. Therefore, a comprehensive state-of-the-art proteomics approach combining subcellular fractionation, shotgun proteomics and spectral counting to assess the relative abundance of proteins within fractions was applied. The analysis of four different proteome fractions (cytosolic, enriched membrane, membrane shaving and extracellular fraction) resulted in the identification of 1582 of the 8270 predicted proteins ... {{/i}}2015-10-21GPM3232001969425896738PXD001497PXD001497