{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Liangying Dai"],"species":["Fragaria Vesca"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0001091000"],"submitter_email":["daily@hunau.net"],"submitter_affiliation":["Hunan Agricultural University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["The various post-translational modifications (PTMs) of plant proteins have important regulatory roles in development. We therefore examined various modified proteins from strawberry stigmata and found that succinylation of lysine residues was the most abundant type of modification. We then subjected proteins from strawberry stigmata to an efficient enrichment method for succinylated peptides and identified 200 uniquely succinylated lysines (Suks) in 116 proteins. A bioinformatics analysis revealed that these proteins are involved in important biological processes, including stress responses, vesicular transport, and energy metabolism. Proteomics, combined with immunoprecipitation and immunoblotting, revealed an obvious increase in succinylation of the assembly polypeptide 2 (AP2) and clathrin from 0.5 to 2 h after pollination, suggesting that succinylation is involved in the recognition of pollen-stigma signaling substances and vesicular transport. These results suggest that AP2/clathrin-mediated vesicular transport processes are regulated by lysine succinylation during pollen recognition."],"pubmed_title":["Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata."],"pubmed_authors":["Fang Xianping X, Xin Ya Y, Sheng Zheliang Z, Liu Hui H, Jiang Aili A, Wang Fang F, Yang Jian J, Xi Xiaojun X, Zha Qian Q, Zhang Liqing L, Dai Liangying L, Yan Chengqi C, Chen Jianping J"],"additional_accession":[]},"is_claimable":false,"name":"Systematic Identification and Analysis of Lysine Succinylation in Strawberry Stigmata","description":"Strawberry is an important economic crop in the world, and strawberry stigmata are vital to promote pollen germination and allow pollen tubes to enter into stigma tissue. Post-translational modification (PTM) of proteins can significantly affect protein structure and function, and plays a very important regulatory role in plant development. So the functional study of PTM during the pollen-stigma recognition process will improve understanding of the molecular mechanism of the pollen-stigma recognition reactions in strawberry plants. However, to date, little is known about the functions of PTM in strawberry stigmata. In this study, we first analyzed various protein PTMs that likely exist in strawberry stigmata, and found lysine succinylation is highly abundant among six types of PTM. Then a highly efficient enrichment method for succinylation peptides was used to identify 209 succinylation modification sites in 120 proteins in the stigmata. Our bioinformatics analysis further revealed that the succinylated proteins are involved in many important biological processes including resistance and stress responses, vesicular transport, as well as processes related to energy and metabolism. Mass spectrometry (MS)-based proteomics, combining with immunoprecipitation (IP) and western blotting (WB), showed that there was anobvious increase in assembly polypeptide 2 (AP2)/clathrin succinylation abundance at 0.5 h or 2 h after pollination, revealing the significance of succinylation in the processes involved in the recognition of pollen-stigma signal substances and vesicular transport. In conclusion, our results suggest that lysine succinylation is involved in many important biological processes, and also reveal that AP2/clathrin-mediated vesicular transport process is strongly regulated by lysine succinylation during pollen recognition. These results suggest novel research directions for studying the molecular mechanism underlying the interaction and recognition between pollen and stigmata in plants, as well as the signal regulatory mechanism.","dates":{"publication":"Fri Dec 15 00:00:00 GMT 2017"},"accession":"PXD008475","cross_references":{"TAXONOMY":["57918"],"pubmed":["30148364"]}}