{"database":"iProX","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":0,"searchCount":0},"additional":{"omics_type":["Proteomics"],"submitter":["Ting Wang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0001907000"],"submitter_email":["twang1@tmu.edu.cn"],"submitter_affiliation":["Tianjin Medical University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["The protein O-GlcNAcylation catalysed by O-GlcNAc transferase (OGT) is tightly regulated by glucose availability. It is upregulated and essential for tumor cell proliferation under hypoxic conditions. However, the mechanism behind is still unclear. Here, we showed that the glycolytic regulator 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3), which also promotes cell cycle progression in the nucleus, was O-GlcNAcylated in response to hypoxia. The O-GlcNAcylation of PFKFB3 could compete phosphorylation by hypoxia-activated ERK at the same modification site Ser172. Phosphorylated PFKFB3 could interact with the protein G3BP2 and retain in the cytosol; this in turn led to the accumulation of hypoxia-induced-P27 in the nucleus resulting in the cell cycle arrest. Such a pathway was compromised by high level of PFKFB3 O-GlcNAcylation in tumor cells contributing to cell cycle progression. Consistently, the PFKFB3-Ser172 phosphorylation level inversely correlated with the OGT level in pancreatic cancer patients. Our findings uncovered an O-GlcNAcylation mediated mechanism to promote tumor cell proliferation under metabolic stress, linking the aberrant OGT activity to tumorigenesis in pancreatic cancer."],"pubmed_title":["O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia."],"pubmed_authors":["Lei Yinrui Y, Chen Tao T, Li Yeyi Y, Shang Man M, Zhang Yan Y, Jin Yuepeng Y, Yu Qiujing Q, Guo Fang F, Wang Ting T"],"citation_count":["0"],"additional_accession":[]},"is_claimable":false,"name":"human PFKFB3 immunoprecipitates LC-MS/MS Raw DATA","description":"Flag-PFKFB3 was expressed in SW1990 cells. Immunoprecipitation analysis was performed using the anti-Flag antibody, and the extracts were analyzed by mass spectrometry.","dates":{"publication":"Mon Dec 09 00:00:00 GMT 2019"},"accession":"PXD016644","cross_references":{"TAXONOMY":["9606"],"pubmed":["32060258"]}}