{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Shengli Zhang"],"species":["Bos Taurus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002230000"],"submitter_email":["zhangslcau@cau.edu.cn"],"submitter_affiliation":["China Agricultural University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["The aim of this study was to understand the molecular mechanisms behind the biological differences of X- and Y-sperm and to screen the sex-specific candidate antigen proteins for sexed semen production. To this end, we investigated differential expression of total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique; 1521 proteins were identified. In the X-sperm group, 8 and 23 proteins were significantly up- and down-regulated, respectively. In the X- and the Y-sperm group, 151 and 88 proteins were specifically expressed, respectively. These were overexpressed in the dynamic changes of the actin cytoskeleton, and cell senescence/apoptosis induced by the immune response, and could result in differences in the state, size, and immune sensitivity of the X-/Y-sperm membranes. The prediction of transmembrane structure, subcellular localization, and Western blotting validation results showed that the CLRN3 and SCAMP1 proteins were cell surface specific antigens of X- and Y-sperm, respectively. Our findings help explain the molecular mechanism behind the biological differences of X-/Y-sperm and lay the foundation for application of immunological methods to produce sex-sorted semen and control livestock sex. Data are available via ProteomeXchange with identifier PXD019435."],"pubmed_title":["Differential Membrane Protein Profile in Bovine X- and Y-Sperm."],"pubmed_authors":["Shen Dan D, Zhou Chenghao C, Cao Mingyue M, Cai Wentao W, Yin Hongwei H, Jiang Li L, Zhang Shengli S"],"additional_accession":[]},"is_claimable":false,"name":"Differential Membrane Protein Profile in Sperm","description":"We investigated the differential expression of the total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique.","dates":{"publication":"Tue May 18 00:00:00 GMT+01:00 2021"},"accession":"PXD019435","cross_references":{"TAXONOMY":["9913"],"pubmed":["34009990"]}}