iProXProteomicsShengli ZhangBos Taurushttp://www.iprox.org/page/project.html?id=IPX0002230000zhangslcau@cau.edu.cnChina Agricultural UniversityiProXThe aim of this study was to understand the molecular mechanisms behind the biological differences of X- and Y-sperm and to screen the sex-specific candidate antigen proteins for sexed semen production. To this end, we investigated differential expression of total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique; 1521 proteins were identified. In the X-sperm group, 8 and 23 proteins were significantly up- and down-regulated, respectively. In the X- and the Y-sperm group, 151 and 88 proteins were specifically expressed, respectively. These were overexpressed in the dynamic changes of the actin cytoskeleton, and cell senescence/apoptosis induced by the immune response, and could result in differences in the state, size, and immune sensitivity of the X-/Y-sperm membranes. The prediction of transmembrane structure, subcellular localization, and Western blotting validation results showed that the CLRN3 and SCAMP1 proteins were cell surface specific antigens of X- and Y-sperm, respectively. Our findings help explain the molecular mechanism behind the biological differences of X-/Y-sperm and lay the foundation for application of immunological methods to produce sex-sorted semen and control livestock sex. Data are available via ProteomeXchange with identifier PXD019435.Differential Membrane Protein Profile in Bovine X- and Y-Sperm.Shen Dan D, Zhou Chenghao C, Cao Mingyue M, Cai Wentao W, Yin Hongwei H, Jiang Li L, Zhang Shengli SfalseDifferential Membrane Protein Profile in SpermWe investigated the differential expression of the total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique.Tue May 18 00:00:00 BST 2021PXD019435991334009990