{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["HuiJing Yi"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002598000"],"submitter_email":["jyhui@sibcb.ac.cn"],"submitter_affiliation":["State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Y-box-binding protein 1 (YB-1) is a multifunctional RNA binding protein involved in virtually every step of RNA metabolism. However, the functions and mechanisms of YB-1 in one of the most aggressive cancers, glioblastoma, are not well understood. In this study, we found that YB-1 protein was markedly overexpressed in glioblastoma and acted as a critical activator of both mTORC1 and mTORC2 signaling. Mechanistically, YB-1 bound the 5'UTR of CCT4 mRNA to promote the translation of CCT4, a component of the CCT chaperone complex, that in turn activated the mTOR signaling pathway by promoting mLST8 folding. In addition, YB-1 autoregulated its own translation by binding to its 5'UTR, leading to sustained activation of mTOR signaling. In patients with glioblastoma, high protein expression of YB-1 correlated with increased expression of CCT4 and mLST8 and activated mTOR signaling. Importantly, the administration of RNA decoys specifically targeting YB-1 in a mouse xenograft model resulted in slower tumor growth and better survival. Taken together, these findings uncover a disrupted proteostasis pathway involving a YB-1/CCT4/mLST8/mTOR axis in promoting glioblastoma growth, suggesting that YB-1 is a potential therapeutic target for the treatment of glioblastoma."],"pubmed_title":["Upregulated YB-1 protein promotes glioblastoma growth through a YB-1/CCT4/mLST8/mTOR pathway."],"pubmed_authors":["Wang Jin-Zhu JZ, Zhu Hong H, You Pu P, Liu Hui H, Wang Wei-Kang WK, Fan Xiaojuan X, Yang Yun Y, Xu Keren K, Zhu Yingfeng Y, Li Qunyi Q, Wu Ping P, Peng Chao C, Wong Catherine Cl CC, Li Kaicheng K, Shi Yufeng Y, Zhang Nu N, Wang Xiuxing X, Zeng Rong R, Huang Ying Y, Yang Liusong L, Wang Zefeng Z, Hui Jingyi J"],"additional_accession":[]},"is_claimable":false,"name":"the function of RNA Binding Protrein in cancer","description":"RNA Binding Protrein plays of vital imortance in cancer progression.","dates":{"publication":"Wed Dec 08 00:00:00 GMT 2021"},"accession":"PXD022776","cross_references":{"TAXONOMY":["9606"],"pubmed":["35239512"]}}