{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Huangying Pang"],"species":["Vibrio Alginolyticus 40b"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002589000"],"submitter_email":["phying1218@163.com"],"submitter_affiliation":["Fisheries College, Guangdong Ocean University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Recent studies have shown that a key strategy of many pathogens is to use post-translational modification (PTMs) to modulate host factors critical for infection. Lysine succinylation (Ksuc) is a major PTM widespread in prokaryotic and eukaryotic cells, and is associated with the regulation of numerous important cellular processes. <i>Vibrio alginolyticus</i> is a common pathogen that causes serious disease problems in aquaculture. Here we used the affinity enrichment method with LC-MS/MS to report the first identification of 2082 lysine succinylation sites on 671 proteins in <i>V. alginolyticus</i>, and compared this with the lysine acetylation of <i>V. alginolyticus</i> in our previous work. The Ksuc modification of SodB and PEPCK proteins were further validated by Co-immunoprecipitation combined with Western blotting. Bioinformatics analysis showed that the identified lysine succinylated proteins are involved in various biological processes and central metabolism pathways. Moreover, a total of 1,005 (25.4%) succinyl sites on 502 (37.3%) proteins were also found to be acetylated, which indicated that an extensive crosstalk between acetylation and succinylation in <i>V. alginolyticus</i> occurs, especially in three central metabolic pathways: glycolysis/gluconeogenesis, TCA cycle, and pyruvate metabolism. Furthermore, we found at least 50 (7.45%) succinylated virulence factors, including LuxS, Tdh, SodB, PEPCK, ClpP, and the Sec system to play an important role in bacterial virulence. Taken together, this systematic analysis provides a basis for further study on the pathophysiological role of lysine succinylation in <i>V. alginolyticus</i> and provides targets for the development of attenuated vaccines."],"pubmed_title":["First Succinylome Profiling of <i>Vibrio alginolyticus</i> Reveals Key Role of Lysine Succinylation in Cellular Metabolism and Virulence."],"pubmed_authors":["Zeng Fuyuan F, Pang Huanying H, Chen Ying Y, Zheng Hongwei H, Li Wanxin W, Ramanathan Srinivasan S, Hoare Rowena R, Monaghan Sean J SJ, Lin Xiangmin X, Jian Jichang J"],"additional_accession":[]},"is_claimable":false,"name":"Succinylationin V alginolyticus virulence","description":"In this project successfully identified a total of 2082 succinylation sites matched with 671 proteins in V. alginolyticus.","dates":{"publication":"Tue Dec 15 00:00:00 GMT 2020"},"accession":"PXD023153","cross_references":{"TAXONOMY":["674977"],"pubmed":["33614530"]}}