{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Catherine CL Wong"],"species":["Severe Acute Respiratory Syndrome Coronavirus 2"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002719000"],"submitter_email":["catherine_wong@bjmu.edu.cn"],"submitter_affiliation":["Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_title":["O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an \"O-Follow-N\" rule."],"pubmed_authors":["Tian Wenmin W, Li Delin D, Zhang Nan N, Bai Guijie G, Yuan Kai K, Xiao Haixia H, Gao Feng F, Chen Yang Y, Wong Catherine C L CCL, Wong Catherine C L CCL, Gao George Fu GF"],"additional_accession":[]},"is_claimable":false,"name":"N- and O-glycosylation patterns of the SARS-CoV-2 spike protein reveal a new “O- follow-N” rule","description":"Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19, has caused a worldwide pandemic since its discovery at the end of 2019. The spike (S) protein of SARS-CoV-2 is responsible for the host cell entry and the critical targets of the immune system. Consequently, S protein has been extensively used in antibody and vaccine studies. Here, we identified a total of 23 N-glycosites, including N334 in the noncanonical consensus motif N-X-C, on the full-length wild-type S protein protomer. Furthermore, 55 O-glycosylation sites were identified, and this uncovered a pattern of O-glycosylation near N-glycosites, which we named the “O-follow-N” rule, revealed by site-specific mutagenesis. The comprehensive mapping of the S protein O- and N-glycosylation landscape provides further information for studying the mechanisms underlying viral pathogenesis and immune evasion.","dates":{"publication":"Wed Mar 24 00:00:00 GMT 2021"},"accession":"PXD023346","cross_references":{"TAXONOMY":["2697049"],"pubmed":["34341488"]}}