iProXProteomicsCatherine CL WongSevere Acute Respiratory Syndrome Coronavirus 2http://www.iprox.org/page/project.html?id=IPX0002719000catherine_wong@bjmu.edu.cnCenter for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking UniversityiProXO-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule.Tian Wenmin W, Li Delin D, Zhang Nan N, Bai Guijie G, Yuan Kai K, Xiao Haixia H, Gao Feng F, Chen Yang Y, Wong Catherine C L CCL, Wong Catherine C L CCL, Gao George Fu GFfalseN- and O-glycosylation patterns of the SARS-CoV-2 spike protein reveal a new “O- follow-N” ruleSevere acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19, has caused a worldwide pandemic since its discovery at the end of 2019. The spike (S) protein of SARS-CoV-2 is responsible for the host cell entry and the critical targets of the immune system. Consequently, S protein has been extensively used in antibody and vaccine studies. Here, we identified a total of 23 N-glycosites, including N334 in the noncanonical consensus motif N-X-C, on the full-length wild-type S protein protomer. Furthermore, 55 O-glycosylation sites were identified, and this uncovered a pattern of O-glycosylation near N-glycosites, which we named the “O-follow-N” rule, revealed by site-specific mutagenesis. The comprehensive mapping of the S protein O- and N-glycosylation landscape provides further information for studying the mechanisms underlying viral pathogenesis and immune evasion.Wed Mar 24 00:00:00 GMT 2021PXD023346269704934341488