{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Heng Yin"],"species":["Arabidopsis Thaliana"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002920000"],"submitter_email":["yinheng@dicp.ac.cn"],"submitter_affiliation":["Dalian Institute of Chemical Physics, Chinese Academy of Sciences."],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["As an essential enzyme in the uridine diphosphate (UDP)-GlcNAc biosynthesis pathway, the significant role of N-acetylglucosamine phosphate mutase (AGM) remains unknown in plants. In the present study, a functional plant AGM (AtAGM) was identified from Arabidopsis thaliana. AtAGM catalyzes the isomerization of GlcNAc-1-P and GlcNAc-6-P, and has broad catalytic activity on different phosphohexoses. UDP-GlcNAc contents were significantly decreased in AtAGM T-DNA insertional mutants, which caused temperature-dependent growth defects in seedlings and vigorous growth in adult plants. Further analysis revealed that protein O-GlcNAcylation but not N-glycosylation was dramatically impaired in Atagm mutants due to UDP-GlcNAc shortage. Combined with the results from O-GlcNAcylation or N-glycosylation deficient mutants, and O-GlcNAcase inhibitor all suggested that protein O-GlcNAcylation impairment mainly leads to the phenotypic variations of Atagm plants. In conclusion, based on the essential role in UDP-GlcNAc biosynthesis, AtAGM is important for plant growth mainly via protein O-GlcNAcylation-level regulation."],"pubmed_title":["Protein O-GlcNAcylation impairment caused by N-acetylglucosamine phosphate mutase deficiency leads to growth variations in Arabidopsis thaliana."],"pubmed_authors":["Jia Xiaochen X, Zhang Hongyan H, Qin Hongqiang H, Li Kuikui K, Liu Xiaoyan X, Wang Wenxia W, Ye Mingliang M, Yin Heng H"],"additional_accession":[]},"is_claimable":false,"name":"Impaired O-GlcNAcylation rather than N-glycosylation caused by AtAGM deficiency leads to growth variations in Arabidopsis thaliana","description":"To reveal the function of N-acetylglucosamine phosphate mutase (AGM) and UDP-GlcNAc in Arabidopsis growth, WT and a T-DNA insertional mutant Atagm-2 were used for proteomic analysis.","dates":{"publication":"Thu Mar 25 00:00:00 GMT 2021"},"accession":"PXD024977","cross_references":{"TAXONOMY":["3702"],"pubmed":["36799458"]}}