iProXProteomicsHeng YinArabidopsis Thalianahttp://www.iprox.org/page/project.html?id=IPX0002920000yinheng@dicp.ac.cnDalian Institute of Chemical Physics, Chinese Academy of Sciences.iProXAs an essential enzyme in the uridine diphosphate (UDP)-GlcNAc biosynthesis pathway, the significant role of N-acetylglucosamine phosphate mutase (AGM) remains unknown in plants. In the present study, a functional plant AGM (AtAGM) was identified from Arabidopsis thaliana. AtAGM catalyzes the isomerization of GlcNAc-1-P and GlcNAc-6-P, and has broad catalytic activity on different phosphohexoses. UDP-GlcNAc contents were significantly decreased in AtAGM T-DNA insertional mutants, which caused temperature-dependent growth defects in seedlings and vigorous growth in adult plants. Further analysis revealed that protein O-GlcNAcylation but not N-glycosylation was dramatically impaired in Atagm mutants due to UDP-GlcNAc shortage. Combined with the results from O-GlcNAcylation or N-glycosylation deficient mutants, and O-GlcNAcase inhibitor all suggested that protein O-GlcNAcylation impairment mainly leads to the phenotypic variations of Atagm plants. In conclusion, based on the essential role in UDP-GlcNAc biosynthesis, AtAGM is important for plant growth mainly via protein O-GlcNAcylation-level regulation.Protein O-GlcNAcylation impairment caused by N-acetylglucosamine phosphate mutase deficiency leads to growth variations in Arabidopsis thaliana.Jia Xiaochen X, Zhang Hongyan H, Qin Hongqiang H, Li Kuikui K, Liu Xiaoyan X, Wang Wenxia W, Ye Mingliang M, Yin Heng HfalseImpaired O-GlcNAcylation rather than N-glycosylation caused by AtAGM deficiency leads to growth variations in Arabidopsis thalianaTo reveal the function of N-acetylglucosamine phosphate mutase (AGM) and UDP-GlcNAc in Arabidopsis growth, WT and a T-DNA insertional mutant Atagm-2 were used for proteomic analysis.Thu Mar 25 00:00:00 GMT 2021PXD024977370236799458