{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Zeyu Sun"],"species":["Severe Acute Respiratory Syndrome Coronavirus 2"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0002757000"],"submitter_email":["zeyusun@zju.edu.cn"],"submitter_affiliation":["Zhejiang University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["The coronavirus disease 2019 (COVID-19) pandemic has led to worldwide efforts to understand the biological traits of the newly identified human coronavirus (HCoV-19) virus. In this mass spectrometry (MS)-based study, we reveal that out of 21 possible glycosites in the HCoV-19 spike protein (S protein), 20 are completely occupied by <i>N</i>-glycans, predominantly of the oligomannose type. All seven glycosylation sites in human angiotensin I converting enzyme 2 (hACE2) were found to be completely occupied, mainly by complex <i>N</i>-glycans. However, glycosylation did not directly contribute to the binding affinity between HCoV-19 S protein and hACE2. Additional post-translational modification (PTM) was identified, including multiple methylated sites in both proteins and multiple sites with hydroxylproline in hACE2. Refined structural models of HCoV-19 S protein and hACE2 were built by adding <i>N</i>-glycan and PTMs to recently published cryogenic electron microscopy structures. The PTM and glycan maps of HCoV-19 S protein and hACE2 provide additional structural details for studying the mechanisms underlying host attachment and the immune response of HCoV-19, as well as knowledge for developing desperately needed remedies and vaccines."],"pubmed_title":["Mass Spectrometry Analysis of Newly Emerging Coronavirus HCoV-19 Spike Protein and Human ACE2 Reveals Camouflaging Glycans and Unique Post-Translational Modifications."],"pubmed_authors":["Sun Zeyu Z, Ren Keyi K, Zhang Xing X, Chen Jinghua J, Jiang Zhengyi Z, Jiang Jing J, Ji Feiyang F, Ouyang Xiaoxi X, Li Lanjuan L"],"additional_accession":[]},"is_claimable":false,"name":"MS characterization of SARS-CoV-2 NCP PTM profile","description":"Glycosylation and PTM profile of NCP from SARS-CoV-2","dates":{"publication":"Wed Apr 14 00:00:00 BST 2021"},"accession":"PXD025404","cross_references":{"TAXONOMY":["2697049"],"pubmed":["32904601"]}}