{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Weimin Zhang"],"species":["Candida Albicans"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0003029000"],"submitter_email":["wmzhang@gdim.cn"],"submitter_affiliation":["Guangdong Institute of Microbiology"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<i>Candida albicans</i> is the main causal pathogen of fungal infections in human beings. Although diverse anti-<i>C. albicans</i> drugs have been explored, the drug resistance and side effects of these drugs are intensifying. Thus, it is urgent to explore new anti-<i>C. albicans</i> compounds from natural products. In this study, we identified trichoderma acid (TA), a compound from <i>Trichoderma spirale</i> with a strong inhibitory effect on <i>C. albicans</i>. Transcriptomic and iTRAQ-based proteomic analyses of TA-treated <i>C. albicans</i> in combination with scanning electronic microscopy and reactive oxygen species (ROS) detection were performed to investigate the potential targets of TA. The most significant differentially expressed genes and proteins after TA treatment were verified through Western blot analysis. Our results revealed that mitochondrial membrane potential, endoplasmic reticulum, ribosomes in the mitochondria, and cell walls were disrupted in TA-treated <i>C. albicans</i>, leading to the accumulation of ROS. The impaired enzymatic activities of superoxide dismutase further contributed to the increase in ROS concentration. The high concentration of ROS led to DNA damage and cell skeleton destruction. The expression levels of Rho-related GTP-binding protein RhoE (RND3), asparagine synthetase (ASNS), glutathione S-transferase, and heat shock protein 70 were significantly up-regulated in response to apoptosis and toxin stimulation. These findings suggest that RND3, ASNS, and supereoxide dismutase 5 are the potential targets of TA, as further demonstrated through Western blot analysis. The combination of transcriptomic, proteomic, and cellular analyses would provide clues for the anti-<i>C. albicans</i> mechanism of TA and the defensive response mechanism of <i>C. albicans</i>. TA is thus recognized as a promising new anti-<i>C. albicans</i> leading compound that alleviates the hazard of <i>C. albicans</i> infection in human beings."],"pubmed_title":["Potential Anti-<i>Candida albicans</i> Mechanism of Trichoderma Acid from <i>Trichoderma spirale</i>."],"pubmed_authors":["Ye Wei W, Chen Yuchan Y, Zhang Weimin W, Liu Taomei T, Liu Yuping Y, Li Mengran M, Li Saini S, Xu Liqiong L, Liu Hongxin H"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome and iTRAQ proteomic analysis reveal the molecular mechanism of anti- Candida albicans action of trichoderma acid, a potent novel drug candidate","description":"Candida albicans has become one of the main pathogens of fungal infection, which causes great threaten to human health. Hence, it is urgent to develop new anti- C. albicans drugs. A compound named as trichoderma acid was isolated from Trichoderma spirale , which showed stronger inhibitory effect towards C. albicans even than positive control nystatin. The transcriptome sequencing and iTRAQ-based sequencing of trichoderma acid-treated C. albicans were performed to investigate the molecular mechanism of anti- C. albicans. And the most significant differential genes were verified by qRT-PCR and Western blot analysis. The results indicated that the disruption of mitochondrial membrane potential, cell membrane, endoplasmic reticulum and the ribosomal in mitochondrial and cell wall were detected in trichoderma acidtreated C. albicans, resulting in the accumulation of ROS. The expression level of supereoxidase SOD was down-regulated, thus enhancing the concentration of ROS. High concentration of ROS led to the DNA damage and destruction of cell skeleton. And the expression levels of Rho-related GTP-binding protein RND3, the asparagine synthetase ASNS, peroxidases, glutathione S-transferase and heat shock protein HSP70 were significantly up-regulated in response to the apoptotic process and toxin stimulus, which was demonstrated by Western blot analysis. The transciptomic, proteomic and cellular analysis would provide anti- C. albicans mechanism of trichoderma acid and the defensive response mechanism of C. albicans.","dates":{"publication":"Thu May 06 00:00:00 GMT+01:00 2021"},"accession":"PXD025834","cross_references":{"TAXONOMY":["5476"],"pubmed":["36982520"]}}