{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Jing Wang"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0003096000"],"submitter_email":["wangjing@ibms.pumc.edu.cn"],"submitter_affiliation":["Peking Union Medical College"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Silicosis is the most prevalent and fatal occupational disease with no effective therapeutics, and currently used drugs cannot reverse the disease progress. Worse still, there are still challenges to be addressed to fully decipher the intricated pathogenesis. Thus, specifying the essential mechanisms and targets in silicosis progression then exploring anti-silicosis pharmacuticals are desperately needed. In this work, multi-omics atlas was constructed to depict the pivotal abnormalities of silicosis and develop targeted agents. By utilizing an unbiased and time-resolved analysis of the transcriptome, proteome and phosphoproteome of a silicosis mouse model, we have verified the significant differences in transcript, protein, kinase activity and signaling pathway level during silicosis progression, in which the importance of essential biological processes such as macrophage activation, chemotaxis, immune cell recruitment and chronic inflammation were emphasized. Notably, the phosphorylation of EGFR (p-EGFR) and SYK (p-SYK) were identified as potential therapeutic targets in the progression of silicosis. To inhibit and validate these targets, we tested fostamatinib (targeting SYK) and Gefitinib (targeting EGFR), and both drugs effectively ameliorated pulmonary dysfunction and inhibited the progression of inflammation and fibrosis. Overall, our drug discovery with multi-omics approach provides novel and viable therapeutic strategies for the treatment of silicosis."],"pubmed_title":["Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration."],"pubmed_authors":["Wang Mingyao M, Zhang Zhe Z, Liu Jiangfeng J, Song Meiyue M, Zhang Tiantian T, Chen Yiling Y, Hu Huiyuan H, Yang Peiran P, Li Bolun B, Song Xiaomin X, Pang Junling J, Xing Yanjiang Y, Cao Zhujie Z, Guo Wenjun W, Yang Hao H, Wang Jing J, Yang Juntao J, Wang Chen C"],"additional_accession":[]},"is_claimable":false,"name":"Proteome and phosphoproteome landscape of silicosis occurrence in mice model","description":"To map the multi-omics atlas of silicosis progression, we collected four replicates of whole lung lobes from mice at different stages (W2, W4, W6, W10) after silica-treated as well as W6 after PBS-treated (as control). Then we performed (phospho) proteome analysis as well as bioinformatic analysis.","dates":{"publication":"Wed Aug 04 00:00:00 BST 2021"},"accession":"PXD027693","cross_references":{"TAXONOMY":["10090"],"pubmed":["35551173"]}}