iProXProteomicsDongfang HuMus Musculushttp://www.iprox.org/page/project.html?id=IPX0003535000dongfang.hu@qq.comHenan Institute of Science and TechnologyiProXFlorfenicol (FLO) has been shown to elicit diverse toxic effects in plants, insects, and mammals. Previously, our investigations revealed that FLO induced abnormal cardiac development and early embryonic mortality in chicken embryos. However, the effect of FLO on mitochondrial responses in stem cells remains unclear. In this study, we show that FLO significantly diminishes proliferation viability and obstructs the directed differentiation of P19 stem cells (P19SCs) into cardiomyocytes. Proteomic analysis revealed 148 differentially expressed proteins in response to FLO. Functional analysis has pinpointed FLO interference with biological processes associated with oxidative phosphorylation within the mitochondria. In alignment with the results of proteomic analysis, we confirmed that FLO inhibits the expression of both nuclear DNA-encoded and mitochondrial DNA-encoded subunits of the electron transport chain. Subsequent experiments demonstrated that FLO disrupts mitochondrial dynamics and induces the mitochondrial unfolded protein response to maintain mitochondrial homeostasis. These findings collectively highlight the significance of mitochondrial dynamics and the mitochondrial unfolded protein response to mediate the decreased proliferation viability and directed differentiation potential in P19SCs treated with FLO. In conclusion, this study provides a comprehensive overview of mitochondrial responses to FLO-induced cytotoxicity and enhances our understandings of the molecular mechanisms underlying FLO-induced embryonic toxicity.Proteomic Analysis of the Mitochondrial Responses in P19 Embryonic Stem Cells Exposed to Florfenicol.Dong Zhihua Z, Hou Xueke X, Wang Xueying X, Shen Zihui Z, Pang Huiqing H, Chen Lingli L, Yin Zhihong Z, Ren Fei F, Li Weiguo W, Ge Yaming Y, Ning Hongmei H, Hu Dongfang DfalseMechanisms underlying Florfenicol-induced stem cell toxicity using iTRAQiTRAQ-based proteomics was performed to explore the mechanism underlying Florfenicol-induced stem cell toxicity and assess the overall protein changes in P19SCs post FLO treatment.Sun Sep 26 00:00:00 GMT+01:00 2021PXD0287641009038133393