{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Shufa Xu"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0003749000"],"submitter_email":["xushufa@caas.cn"],"submitter_affiliation":["Institute of Apicultural Research, Chinese Academy of Agricultural Sciences"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["10-Hydroxydec-2-enoic acid (10-HDA), an unsaturated hydroxyl fatty acid from the natural food royal jelly, can protect against cell and tissue damage, yet the underlying mechanisms are still unexplored. We hypothesized that the neutralization of the hydroxyl free radical (•OH), the most reactive oxygen species, is an important factor underlying the cytoprotective effect of 10-HDA. In this study, we found that the •OH scavenging rate by 10-HDA (2%, g/ml) was more than 20%, which was achieved through multiple-step oxidization of the -OH group and C=C bond of 10-HDA. Moreover, 10-HDA significantly enhanced the viability of vascular smooth muscle cells (VSMCs) damaged by •OH (<i>P</i> < 0.01), significantly attenuated •OH-derived malondialdehyde production that represents cellular lipid peroxidation (<i>P</i> < 0.05), and significantly increased the glutathione levels in •OH-stressed VSMCs (<i>P</i> < 0.05), indicating the role of 10-HDA in reducing •OH-induced cytotoxicity. Further proteomic analyses of VSMCs identified 195 proteins with decreased expression by •OH challenge that were upregulated by 10-HDA rescue and were primarily involved in protein synthesis (such as translation, protein transport, ribosome, and RNA binding) and energy metabolism (such as fatty acid degradation and glycolysis/gluconeogenesis). Taken together, these findings indicate that 10-HDA can effectively promote cell survival by antagonizing •OH-induced injury in VSMCs. To the best of our knowledge, our results provide the first concrete evidence that 10-HDA-scavenged •OH could be a potential pharmacological application for maintaining vascular health."],"pubmed_title":["10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism."],"pubmed_authors":["Fan Pei P, Sha Fangfang F, Ma Chuan C, Wei Qiaohong Q, Zhou Yaqi Y, Shi Jing J, Fu Jiaojiao J, Zhang Lu L, Han Bin B, Li Jianke J"],"additional_accession":[]},"is_claimable":false,"name":"10-HDA reduces the hydroxyl free radical-induced damage to vascular smooth muscle cells by rescuing protein and energy metabolisms","description":"10-hydroxydec-2-enoic acid (10-HDA), a natural agent from royal jelly, could fight against cell and tissue damage, yet the underlying mechanisms are still unexplored. Here, we found the scavenging rate of hydroxyl free radical (•OH) by 2% (g/mL) 10-HDA was more than 20%, which was achieved by a multiple-step oxidization of the −OH group and C=C bond in 10-HDA. Moreover, 10-HDA could enhance the viability of vascular smooth muscle cells (VSMCs) damaged by •OH, indicating its role in reducing the •OH-induced cytotoxicity. The 195 proteins decreased by •OH challenge in VSMCs were up-regulated by 10-HDA rescue, which mainly elevated the activities of protein and energy metabolisms within nucleus and cytoplasm. These evidences indicate that 10-HDA plays key roles in promoting cell survival through antagonizing •OH-induced injury in VSMCs. Our results provide the first solid evidence that 10-HDA scavenging •OH could be a potential pharmacological application in keeping vascular health.","dates":{"publication":"Mon Nov 22 00:00:00 GMT 2021"},"accession":"PXD029873","cross_references":{"TAXONOMY":["10090"],"pubmed":["35711556"]}}