iProXProteomicsDingqiang ChenKlebsiella Pneumoniae Jh1http://www.iprox.org/page/project.html?id=IPX0003841000jyksys@126.comZhuJiang Hospital of Southern Medical UniversityiProXCarbapenem-resistant Klebsiella pneumoniae (CRKP) is an urgent threat to human health. Major outer membrane proteins (OMPs) porin mutation is one important resistance mechanism of CRKP, and may also affect the inhibition activity of β-lactam and β-lactamase inhibitor combinations. The ertapenem-resistant K. pneumoniae strain 2018B120 with major porin mutations was isolated from a clinical patient. Genomic and time-series proteomic analyses were conducted to retrieve the ertapenem-challenged response of 2018B120. The abundance changing of proteins from PTS systems,  ABC transporters, the autoinducer 2 (AI-2) quorum sensing system, and antioxidant systems can be observed. Overexpression of alternative porins was also noticed to balance major porins' defection. These findings added a detailed regulation network in bacterial resistance mechanisms and gave new insights into bypass adaptation mechanisms the porin deficient bacteria adopted under carbapenem antibiotics pressure. SIGNIFICANCE: Outer membrane porins deficiency is an important mechanism of carbapenem resistance in K. pneumoniae. Comprehensive genomic and proteomic profiling of an ertapenem-resistant K. pneumoniae strain 2018B120 gives a detailed systematic regulation network in bacterial resistance mechanisms. Overexpression of alternative porins to balance major porins' defection was noticed, giving new insights into bypass adaptation mechanisms of porin deficient bacteria.Proteomics profiling of ertapenem challenged major porin deficient carbapenem-resistant Klebsiella pneumoniae.Yuan Pei-Bo PB, Ling Jia-Hui JH, Zhu Jia-Hui JH, Peng Chen C, Chen En-Zhong EZ, Zhong Yu-Xia YX, Liu Wan-Ting WT, Wang Lin-Jing LJ, Yang Ling L, Chen Ding-Qiang DQfalseProteomics profiling of major porin deficient Klebsiella pneumoniae in response to ertapenem treatmentThe ertapenem-resistant K. pneumoniae strain 2018B120 with major porin loss was isolated from a clinical patient, comprehensive genomics and proteomics analysis were conducted.Mon Aug 14 00:00:00 BST 2023PXD030432103789036058541