iProXProteomicsYang ZhangHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0003967000zhangyang@fudan.edu.cnInstitutes of Biomedical Sciences, Fudan UniversityiProXTrichosanthin (TCS) is a traditional Chinese herbal medicine used to treat some gynecological diseases. Its effective component has diverse biological functions, including antineoplastic activity. The human trophoblast cell line BeWo was chosen as an experimental model for in vitro testing of a drug screen for anticancer properties of TCS. The MTT method was used in this study to get a primary screen result. The result showed that 100 mM had the best IC₅₀ value. Proteomics analysis was then performed for further investigation of the drug effect of TCS on the BeWo cell line. In this differential proteomic expression analysis, the total proteins extracted from the BeWo cell line and their protein expression level after the drug treatment were compared by 2DE. Then, 24 unique three-fold differentially expressed proteins (DEPs) were successfully identified by MALDI-TOF/TOF MS. Label-free proteomics was run as a complemental method for the same experimental procedure. There are two proteins that were identified in both the 2DE and label-free methods. Among those identified proteins, bioinformatics analysis showed the importance of pathway and signal transduction and gives us the potential possibility for the disease treatment hypothesis.Comparative Proteomic Analysis of Drug Trichosanthin Addition to BeWo Cell Line.Hu Yajun Y, Yao Jun J, Wang Zening Z, Liang Hui H, Li Cunyu C, Zhou Xinwen X, Yang Fengying F, Zhang Yang Y, Jin Hong HfalseComparative proteomic analysis of drug trichosanthin addition to BeWo cell lineTrichosanthin (TCS) is a traditional Chinese herbal medicine to treat some gynecological diseases. Its effective component has diverse biological function, including antineoplastic activity. The human trophoblast cell line BeWo was chosen as an experimental model for in vitro test of drug screen for anti-cancer property of TCS. MTT method was used in this study to get primary screen result. The result showed that 100 mM had the best IC50 value. Proteomics analysis was then performed for further investigation for the drug effect of TCS to the BeWo cell line. In this differential proteomic expression analysis, total protein extract from BeWo cell line and their protein expression level after the drug treatment were compared by 2DE. Then 24 unique three-fold differential expressed proteins (DEPs) were successfully identified by MALDI-TOF-TOF-MS. Label-free proteomics as complemental method was run for the same experimental procedure. There are two proteins were identified in both 2DE and label free methods. Among those identified proteins, bioinformatics analysis shows the importance of pathway and signal transduction and gives us the potential possibility for the disease treatment hypothesis.Thu Jan 13 00:00:00 GMT 2022PXD030920960635268705