{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Jiayi Wang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0004298000"],"submitter_email":["karajan2@163.com"],"submitter_affiliation":["Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["N<sup>6</sup>-Methyladenosine (m<sup>6</sup>A) RNA modification, methylation at the N6 position of adenosine, plays critical roles in tumorigenesis. m<sup>6</sup>A readers recognize m<sup>6</sup>A modifications and thus act as key executors for the biological consequences of RNA methylation. However, knowledge about the regulatory mechanism(s) of m<sup>6</sup>A readers is extremely limited. In this study, RN7SK was identified as a small nuclear RNA that interacts with m<sup>6</sup>A readers. m<sup>6</sup>A readers recognized and facilitated secondary structure formation of m<sup>6</sup>A-modified RN7SK, which in turn prevented m<sup>6</sup>A reader mRNA degradation from exonucleases. Thus, a positive feedback circuit between RN7SK and m<sup>6</sup>A readers is established in tumor cells. From findings on the interaction with RN7SK, new m<sup>6</sup>A readers, such as EWS RNA binding protein 1 (EWSR1) and KH RNA binding domain containing, signal transduction-associated 1 (KHDRBS1), were identified and shown to boost Wnt/β-catenin signaling and tumorigenesis by suppressing translation of Cullin1 (CUL1). Moreover, several Food and Drug Administration-approved small molecules were demonstrated to reduce RN7SK expression and inhibit tumorigenesis. Together, these findings reveal a common regulatory mechanism of m<sup>6</sup>A readers and indicate that targeting RN7SK has strong potential for tumor treatment."],"pubmed_title":["A positive feedback circuit between RN7SK snRNA and m<sup>6</sup>A readers is essential for tumorigenesis."],"pubmed_authors":["Xu Xin X, Ma Lifang L, Zhang Xiao X, Guo Susu S, Guo Wanxin W, Wang Yikun Y, Qiu Shiyu S, Tian Xiaoting X, Miao Yayou Y, Yu Yongchun Y, Wang Jiayi J"],"additional_accession":[]},"is_claimable":false,"name":"Mass spectrometry for the proteomics following RNA pull down of m6A methylated RN7SK in lung adenocarcinoma A549 cell line.","description":"The aim of this project was to investigate the proteins that bind to m6A methylated RN7SK in lung adenocarcinoma A549 cell line. And mass spectrometry of A549 cell treated with or without m6A methylated RN7SK in three replicates.","dates":{"publication":"Tue Apr 12 00:00:00 BST 2022"},"accession":"PXD033184","cross_references":{"TAXONOMY":["9606"],"pubmed":["36566349"]}}