{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Xiaomin Zhang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0005033000"],"submitter_email":["xzhang08@tmu.edu.cn"],"submitter_affiliation":["Tianjin Medical University Eye Hospital"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<h4>Background</h4>Uveitis and posterior scleritis are sight-threatening diseases with undefined pathogenesis and accurate diagnosis remains challenging.<h4>Methods</h4>Two plasma-derived extracellular vesicle (EV) subpopulations, small and large EVs, obtained from patients with ankylosing spondylitis-related uveitis, Behcet's disease uveitis, Vogt-Koyanagi-Harada syndrome, and posterior scleritis were subjected to proteomics analysis alongside plasma using SWATH-MS. A comprehensive bioinformatics analysis was performed on the proteomic profiles of sEVs, lEVs, and plasma. Candidate biomarkers were validated in a new cohort using ELISA. Pearson correlation analysis was performed to analyze the relationship between clinical parameters and proteomic data. Connectivity map database was used to predict therapeutic agents.<h4>Results</h4>In total, 3,668 proteins were identified and over 3000 proteins were quantified from 278 samples. When comparing diseased group to healthy control, the proteomic profiles of the two EV subgroups were more correlated with disease than plasma. Comprehensive bioinformatics analysis highlighted potential pathogenic mechanisms for these diseases. Potential biomarker panels for four diseases were identified and validated. We found a negative correlation between plasma endothelin-converting enzyme 1 level and mean retinal thickness. Potential therapeutic drugs were proposed, and their targets were identified.<h4>Conclusions</h4>This study provides a proteomic landscape of plasma and EVs involved in ankylosing spondylitis-related uveitis, Behcet's disease uveitis, Vogt-Koyanagi-Harada syndrome, and posterior scleritis, offers insights into disease pathogenesis, identifies valuable biomarker candidates, and proposes promising therapeutic agents."],"pubmed_title":["Comprehensive profiling of extracellular vesicles in uveitis and scleritis enables biomarker discovery and mechanism exploration."],"pubmed_authors":["Wu Lingzi L, Zhou Lei L, An Jinying J, Shao Xianfeng X, Zhang Hui H, Wang Chunxi C, Zhao Guixia G, Chen Shuang S, Cui Xuexue X, Zhang Xinyi X, Yang Fuhua F, Li Xiaorong X, Zhang Xiaomin X"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic landscape of plasma and plasma-derived sEVs and lEVs in uveitis and posterior scleritis","description":"Uveitis and posterior scleritis are sight-threatening diseases with undefined pathogenesis, accurate diagnosis remains challenging. Here, two plasma-derived extracellular vesicle (EV) subpopulations, small and large EVs, were obtained from patients with uveitis and posterior scleritis and subjected to proteomics analysis alongside plasma. We identify 3,658 proteins, with 3,097, 3,312, and 2,820 quantifiable proteins for sEVs, lEVs, and plasma, respectively. The proteomes of the two EV subgroups differed significantly and were more highly correlated with disease development than that of plasma. Comprehensive bioinformatics analysis of the EVs and plasma highlighted a potential pathogenic mechanism for these diseases. Four potential biomarker panels for four diseases were identified and validated. We found a negative correlation between plasma endothelin-converting enzyme 1 levels and mean retinal thickness. This study provides a proteomic landscape of the molecular changes in plasma and EVs involved in four diseases, offers insights into their potential pathogenesis, and identifies valuable biomarker candidates.","dates":{"publication":"Mon Sep 19 00:00:00 BST 2022"},"accession":"PXD036844","cross_references":{"TAXONOMY":["9606"],"pubmed":["37322475"]}}