iProXProteomicsWei SongHomo SapiensMus Musculushttp://www.iprox.org/page/project.html?id=IPX0005384000roy_sw0925@sina.comInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical CollegeiProXRNA-binding proteins (RBPs), as key regulators of mRNA fate, are abundantly expressed in the testis. However, RBPs associated with human male infertility remain largely unknown. Through bioinformatic analyses, we identified 62 such RBPs, including an evolutionarily conserved RBP, DEAD-box helicase 20 (DDX20). Male germ-cell-specific inactivation of Ddx20 at E15.5 caused T1-propsermatogonia (T1-ProSG) to fail to reenter cell cycle during the first week of testicular development in mice. Consequently, neither the foundational spermatogonial stem cell (SSC) pool nor progenitor spermatogonia were ever formed in the knockout testes. Mechanistically, DDX20 functions to control the translation of its target mRNAs, many of which encode cell-cycle-related regulators, by interacting with key components of the translational machinery in prospermatogonia. Our data demonstrate a previously unreported function of DDX20 as a translational regulator of critical cell-cycle-related genes, which is essential for cell-cycle reentry of T1-ProSG and formation of the SSC pool.DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice.Zou Dingfeng D, Li Kai K, Su Luying L, Liu Jun J, Lu Yan Y, Huang Rong R, Li Mengzhen M, Mang Xinyu X, Geng Qi Q, Li Pengyu P, Tang Jielin J, Yu Zhixin Z, Zhang Zexuan Z, Chen Dingyao D, Miao Shiying S, Yu Jia J, Yan Wei W, Song Wei WfalseMS analysis of endogenously precipitated DDX20 protein complexes (immunoprecipitation (IP)–MS)Protein lysates were derived from HEK293T cells and spermatogonial stem cells，and interacting proteins were “pulled-down” for analysis by gel electrophoresis and identification by mass spectrometry. An equal amount of non-specific IgG from rabbit serum (Thermos Fisher Scientific) was used as a negative control.Wed Apr 24 00:00:00 GMT+01:00 2024PXD03817110090960638657611