iProXProteomicsKunlun HeHuman Parechovirus Strain T82-0169http://www.iprox.org/page/project.html?id=IPX0005258000hekl301@163.comChinese PLA general hospitaliProX<b>Introduction:</b> We aimed to identify urine biomarkers for screening individuals with adaptability to high-altitude hypoxia with high stamina levels. Although most non-high-altitude natives experience rapid decline in physical ability when ascending to high altitudes, some individuals with high-altitude adaptability continue to maintain high endurance levels. <b>Methods:</b> We divided the study population into two groups: the LC group (low change in endurance from low to high altitude) and HC group (high change in endurance from low to high altitude). We performed blood biochemistry testing for individuals at high altitudes and sea level. We used urine peptidome profiling to compare the HH (high-altitude with high stamina) and HL (high-altitude with low stamina) groups and the LC and HC groups to identify urine biomarkers. <b>Results:</b> Routine blood tests revealed that the concentration of white blood cells, lymphocytes and platelets were significantly higher in the HH group than in the HL group. Urine peptidome profiling showed that the proteins ITIH1, PDCD1LG2, NME1-NME2, and CSPG4 were significantly differentially expressed between the HH and HL groups, which was tested using ELISA. Urine proteomic analysis showed that LRG1, NID1, VASN, GPX3, ACP2, and PRSS8 were urine proteomic biomarkers of high stamina during high-altitude adaptation. <b>Conclusion:</b> This study provides a novel approach for identifying potential biomarkers for screening individuals who can adapt to high altitudes with high stamina.Identification of novel urine proteomic biomarkers for high stamina in high-altitude adaptation.Liu Chunlei C, Guo Ge G, Li Xin X, Shen Yanying Y, Xu Xiang X, Chen Yibing Y, Li Hanlu H, Hao Jianxiu J, He Kunlun KfalseIdentification of novel urine proteomic biomarkers for high stamina in high-altitude adaptationAscending to high-altitude of non-high-altitude natives showed rapidly declined physical ability, but some with high-altitude adaptability were still able to maintain a high level. Our goal was to find urine biomarkers that could be used to assess people for high-altitude hypoxia adaptation with high stamina. Blood routine tests were done for HH (High-altitude with high stamina) and HL (High-altitude with low stamina) groups at high altitude and at sea level. By contrasting urine peptidome profiling between the HH and HL groups, urine biomarkers were discovered. Blood routine tests showed that WBC, LYM, and PLT concentrations were considerably greater in HH than in HL. Four proteins have significantly different levels of expression between HH and HL, according to urine peptidome profiling. Expression of these proteins were tested by ELISA in HH and HL samples. Our study provided a novel approach for identifying potential biomarkers for screening people who can adapt to high altitudes with high stamina.Thu Feb 02 00:00:00 GMT 2023PXD039791100105637250129