{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Ying Gai"],"species":["Larix"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0005981000"],"submitter_email":["gaiying@bjfu.edu.cn"],"submitter_affiliation":["Beijing Forestry University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<i>Larix olgensis</i> is an economically important tree species native to northeastern China. The use of somatic embryogenesis (SE) is efficient and enables the rapid production of varieties with desirable qualities. Here, isobaric labeling via tandem mass tags was used to conduct a large-scale quantitative proteomic analysis of proteins in three critically important stages of SE in <i>L. olgensis</i>: the primary embryogenic callus, the single embryo, and the cotyledon embryo. We identified 6269 proteins, including 176 shared differentially expressed proteins across the three groups. Many of these proteins are involved in glycolipid metabolism, hormone response/signal transduction, cell synthesis and differentiation, and water transport; proteins involved in stress resistance and secondary metabolism, as well as transcription factors, play key regulatory roles in SE. The results of this study provide new insights into the key pathways and proteins involved in SE in <i>Larix</i>. Our findings have implications for the expression of totipotency, the preparation of synthetic seeds, and genetic transformation."],"pubmed_title":["Large-Scale Quantitative Proteomic Analysis during Different Stages of Somatic Embryogenesis in <i>Larix olgensis</i>."],"pubmed_authors":["Hou Jiayin J, Wang Xuechun X, Liu Weifeng W, Jiang Xiangning X, Gai Ying Y"],"additional_accession":[]},"is_claimable":false,"name":"Large-scale Quantitative Proteomics Analysis During Different Stages of Somatic Embryogenesis in Larix olgensis","description":"Larix olgensis is an economically important tree species native to northeastern China. Its somatic embryogenesis (SE) system is both efficient and stable, and this permits varieties with desirable qualities to be reproduced rapidly. Here, isobaric labeling via tandem mass tags was used to conduct a large-scale quantitative proteomics analysis of proteins in three critically important stages of SE in L. olgensis: the primary embryogenic callus, single embryo, and cotyledon embryo. We identified 6,269 proteins, including 176 shared differentially expressed proteins across the three groups. Many of these proteins were involved in glycolipid metabolism, hormone response/signal transduction, cell synthesis and differentiation, and water transport; proteins involved in stress resistance and secondary metabolism, as well as transcription factors, play key regulatory roles in SE. The results of this study provide new insights into the key pathways and proteins involved in SE in Larix. Our findings have implications f","dates":{"publication":"Fri Feb 24 00:00:00 GMT 2023"},"accession":"PXD040378","cross_references":{"TAXONOMY":["3325"],"pubmed":["36975500"]}}