iProXProteomicsHui JiangMus Musculushttp://www.iprox.org/page/project.html?id=IPX0006068000jianghui@ahtcm.edu.cnExperimental Center of Clinical Research, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, ChinaiProX<h4>Background</h4>Liver fibrosis (LF) is a kind of progressive liver injury reaction. The goal of this study was to achieve a more detailed understanding of the molecular changes in response to CCl₄-induced LF through the identification of a differentially expressed liver transcriptomic and proteomic.<h4>Results</h4>A total of 1224 differentially expressed genes (DEGs) and 302 differentially expressed proteins (DEPs) were significantly identified at the transcriptomic and proteomic level, respectively, and 69 genes (hereafter "cor-DEGs-DEPs" genes) were detected at both levels. Pathway enrichment analysis showed that these cor-DEGs-DEPs genes were significantly enriched in 133 pathways. Importantly, among the cor-DEGs-DEPs genes, Gstm1, Gstm3, Ephx1 and Gstp1 were shown to be associated with metabolic pathways, and confirmed by RT-qPCR and parallel reaction monitoring (PRM) verification.<h4>Conclusions</h4>Through the combined analysis of transcriptomic and proteomic data, this study provides valuable insights into the potential mechanism of the pathogenesis of LF, and lays a theoretical foundation for the further development of targeted therapy for LF.Liver transcriptomic and proteomic analyses provide new insight into the pathogenesis of liver fibrosis in mice.Zhang Lili L, Zhou Qiumei Q, Zhang Jiafu J, Cao Kefeng K, Fan Chang C, Chen Sen S, Jiang Hui H, Wu Furong FfalseGlobal quantitative analysis of proteomic of mice with liver fibrosis based on DIA and PRM technologyLiver fibrosis (LF) is a kind of progressive liver injury reaction. The goal of this study was to achieve a more detailed understanding of the molecular changes in response to CCl4-induced LF through the identification of a differentially expressed liver proteomic. A total of 302 differentially expressed proteins (DEPs) were significantly identified at the proteomic level. Importantly,Gstm1, Gstm3, Ephx1 and Gstp1 were shown to be associated with metabolic pathways, and parallel reaction monitoring (PRM) verification showed that the results were consistent with those of data -independent acquisition (DIA). Through the analysis of proteomic data, this study provides valuable insights into the potential mechanism of the pathogenesis of LF, and lays a theoretical foundation for the further development of targeted therapy for LF.Tue Mar 14 00:00:00 GMT 2023PXD0408761009037918454