iProXProteomicsZewen WenStaphylococcus Aureushttp://www.iprox.org/page/project.html?id=IPX0006096000wenzw05@163.comNanshan HospitaliProX0.00.00.00.00.0Comparative Studies, 6-trifluoro-4-((5S)-5-((3-isoxazolylamino)methyl)-2-oxo-3-oxazolidinyl)phenyl)-, Controlling, DMSO, DIMETHYL SULFOXIDE, grupo, dimethyli sulfoxidum, U-100766, dmso, Peptidomics, 3-dihydro-1-(2, protein complex, Zyvox, methylsulfinylmethane, number, dimethylsulfoxyde, protein, protein-containing complex, presence, 5-trifluoro-4-(4-oxo-3, group, (CH3)2SO, count in organism, protein polypeptide chains, native protein, natural protein, polypeptide chain, Protein, 2, U 100766, group., 3, PNU100766, Dimethyl sulfoxide, Biofilm, protein aggregate, Staphlococcus pyogenes citreus, S(O)Me2, sulfinylbis(methane), (methanesulfinyl)methane, Staphylococcus aureus subsp. anaerobius, reference sample, 5-((isoxazol-3-ylamino)methyl)-3-(2, Staphilococcus aureus, 4(1H)-Pyridinone, dimethyl sulphoxide, Rest, N-((3-(3-fluoro-4-morpholinylphenyl)-2-oxo-5-oxazolidinyl)methyl)acetamide, dimethyl sulfoxide, U, Micrococcus aureus, proteins, Streptococcus aureus, dimetil sulfoxido, Micrococcus pyogenes, Staphylococcus pyogenes aureus, grupos, MRX-I compound, U100766, 4-dihydropyridin-1(2H)-yl)phenyl)oxazolidin-2-one, Linezolide, Staphylococus aureus, quantitative, dimethyl sulfur oxide, groupe, Dimethylsulfoxid, 100766, PNU-100766, Controlled, Gruppe, PNU 100766, presence or absence in organismDIMETHYL SULFOXIDE, Tandem, determination, Experiment., Zyvox, SDH, protein, lysis, Mass Spectrometry Mass Spectrometry, Alkylations, dmTAF[[II]]230, SDS, Background, protein polypeptide chains, deoxycholate, Cultural, 2, 3, Min, protein aggregate, DmelCG42273, ethnicity, Eubacteria, reference sample, sarcoma of breast, TFIID TAF250, cel, DmelCG13176, dimethyl sulphoxide, min, ORF19, U, mAPC, IBP, proteins, Mass Spectrometry, free, AI047805, Bacteria <bacteria>, SOLO DANCERS, Prokaryotae, anatomical tube, Procaryotae, autolysin activity, necrosis, 12alpha-dihydroxy-5beta-cholanate, Bladder pain syndrome, Dimethylsulfoxid, PNU-100766, Tube, DMSO, dTAF[[II]]230, dimethyli sulfoxidum, BZRP, U-100766, dmso, 3-dihydro-1-(2, methylsulfinylmethane, Liquid Chromatography, Dmel_CG7826, TAF200, AI836084, TAFII-250, breast sarcoma, TAF250/230, 5-trifluoro-4-(4-oxo-3, PBS, PBR, TUBE, (CH3)2SO, Cultural Background, TAFII250, Cultures, Dm1, bacteriocin activity, prokaryotes, PNU100766, 1110049F14Rik, WASH, Biofilm, Dmel_CG7835, Hydrogen Oxide, Mnb, MNB, (methanesulfinyl)methane, Painful Bladder Syndrome, MS/MS, 4(1H)-Pyridinone, AW124434, CG17603, TAF[[II]], lysin activity, Painful bladder syndrome, Taf250, MRX-I compound, SR3-5, duct, bacteriolytic toxin activity, U100766, Prokaryota, TAF230, Gruppe, Bladder Pain Syndrome, BPBS, F10B6_15, Beliefs, d230, sarcoma of the breast, number, dimethylsulfoxyde, dTAFII250, eubacteria, protein-containing complex, EfW1, presence, CG7826, F10B6.15, Buffer, polypeptide chain, dmTAF1, Taf230, p63, p65, CG7835, CG42273, U 100766, Backgrounds, S(O)Me2, TAF250, sulfinylbis(methane), Taf200, FAM39E, dTAF[[II]]250, 2310061K05Rik, 5-((isoxazol-3-ylamino)methyl)-3-(2, cell, MS2, N-((3-(3-fluoro-4-morpholinylphenyl)-2-oxo-5-oxazolidinyl)methyl)acetamide, 4733401P19Rik, Cultural Relativisms, Taf1p, pk18, ESI, Monera, MBR, dimetil sulfoxido, dTAF250, grupos, Customs, PTBR, Linezolide, fungi, culture, TAF, Controlled, PNU 100766, 6-trifluoro-4-((5S)-5-((3-isoxazolylamino)methyl)-2-oxo-3-oxazolidinyl)phenyl)-, Controlling, DYRK1, bacteria, TAF[[II]]250, grupo, bead, DBI, protein complex, l(3)84Ab, BG:DS00004.13, Cultural Backgrounds, buffer, 3alpha, Cell, group, Belief, dTAF230, Wash2, count in organism, Wash1, native protein, PKBS, natural protein, p230, 12alpha-dihydroxy-5beta-cholan-24-oate, Protein, chemical analysis, AI551343, TAF[[II]]250/230, TFIID, Dyrk1, Dimethyl sulfoxide, dJ142L7.2, tandem MS, CG13176, AU020952, Taf[[II]]250, TAF[[II]]230, holin, CC1, Rest, dimethyl sulfoxide, SWDS, TAF[II]250, Mass Spectrometry-Mass Spectrometry, CGI-97, mDRC, ME-IV, Desoxycholat, DmelCG17603, prokaryote, Relativisms, 4-dihydropyridin-1(2H)-yl)phenyl)oxazolidin-2-one, assay, quantitative, Relativism, dimethyl sulfur oxide, Cultural Relativism, groupe, 100766, TAF1, presence or absence in organismfalse1/4 MIC Contezolid, 1/4 MIC Linezolid and DMSO control group of Staphylococcus aureus biofilm quantitative proteomics comparative study to observe the global expression of protein Contezolid, Linezolid treatment groups and DMSO control groupS. aureus isolate in stable phase were inoculated into 15 ml TSBG plate culture, which contained 1/4 MIC of contezolid and linezolid treatment (DMSO as control) with 3 technical replicates in each group,. Incubate at 37 ° for 16 h and centrifuge at 5,000 rpm for 5 min to collect bacteria, including the formed biofilm bacteria. Wash with 1 ml PBS in water twice, transferred to a 1.5 ml EP tube, centrifuge and discard the supernatant. Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.Thu Mar 16 00:00:00 GMT 2023PXD0409231280