iProXProteomicsLuyang YuHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0006849000luyangyu@zju.edu.cnZhejiang UniversityiProXPerinatal stem cells have prominent applications in cell therapy and regenerative medicine. Among them, human Wharton's jelly mesenchymal stem/stromal cells (hWJMSCs) and human amniotic epithelial stem cells (hAESCs) have been widely used. However, the distinction in the therapeutic potential of hWJMSCs and hAESCs is poorly understood. In this study, we reported the phenotypic differences between these two distinct cell types and provided the first systematic comparison of their therapeutic potential in terms of immunomodulation, extracellular matrix (ECM) remodelling, angiogenesis and antioxidative stress using proteomics. The results revealed that the two cell types presented different protein expression profiles and were both promising candidates for cell therapy. Both types of cells demonstrated angiogenic and antifibrotic potential, whereas hAESCs presented superior immunological tolerance and antioxidant properties, which were supported by a series of relevant in vitro assays. Our study provides clues for the selection of appropriate cell types for diverse indications in cell therapy, which contributes to the advancement of their clinical translation and application.Proteomic analysis of human Wharton's jelly mesenchymal stem/stromal cells and human amniotic epithelial stem cells: a comparison of therapeutic potential.Ge Zhen Z, Qiu Chen C, Zhou Jiayi J, Yang Zhuoheng Z, Jiang Tuoying T, Yuan Weixin W, Yu Luyang L, Li Jinying JWhartons Jelly, Mother Cell, Therapy, Colony Forming Unit, human being, Man (Taxonomy), Wharton's, Stromal, axis, Stem, Modern, Progenitor Cells, Colony-Forming Units, Progenitor Cell, Mother, stalk, Colony Forming Units, Treatments., Scanning Transmission, human, culm, Cell, Wharton's Jelly, Human, umbilical cord mesenchyme, Electron Microscopy, Colony-Forming Unit, Homo sapiens, primary axis, epithelial, Stem Cell, Therapeutic, Stromal Cell, Modern Man, Cells, proteomic analysis, STEM, Mother Cells, Therapies, Scanning Transmission 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protein biosynthesis, Immunomodulatory Therapy, DNA Oxidative Damages, techniques, neurotrophin receptor signaling pathway, Wharton's jelly, epitheliocyte, little, Mother Cell, Anti Oxidant Effects, antioxidants, Anti-oxidative Stress, DNA Oxidative Damage, Stem, Colony Forming Units, TNFalpha, Methodological, Cleavage, Immunomodulatory Therapies, Methodological Study, Treatments, cell_type, human, Nitro-Oxidative, Protein Gene Products, Gene Proteins, Antioxidant Effects, DmelCG6794, Endogenous Antioxidants, Tolerance, primary axis, Therapeutic, Modern Man, Cells, Stress, STEM, Regenerative Medicines, Tnfa, Treatment, Oxidative Stress Injury, DNA, MMRN, Nitro Oxidative Stress, Jelly, Tnfsf1a, methodology, Oxidative DamagefalseProteomic analysis of human Wharton's jelly mesenchymal stem/stromal cells and human amniotic epithelial stem cells: a comparison of therapeutic potentialBackground: Perinatal stem cells have prominent applications in cell therapy and regenerative medicine. Among them, human Wharton's jelly mesenchymal stem/stromal cells (hWJMSCs) and human amniotic epithelial stem cells (hAESCs) are widely used. However, little is known about the dif-ferences in the therapeutic potential of hWJMSCs and hAESCs. Methods: In this study, we reported the phenotypic differences between the two cells and provided the first systematic comparison of their therapeutic potential in terms of immunomodulation, extracellular matrix (ECM) remod-eling, angiogenesis and anti-oxidative stress by means of proteomics. Results: The results showed that the two cells had different protein expression profiles and were both promising candidates for cell therapy. hWJMSCs had a stronger angiogenic potential, while hAESCs had advantages in terms of immune tolerance and antioxidant properties. Notably, hAESCs were found to be enriched in the neurotrophin signaling pathway. Conclusions: Our study provides clues on the choice of cell type in cell therapy for different indications, which will be beneficial in advancing their further clinical translation and application.Wed Aug 02 00:00:00 GMT+01:00 2023PXD044276960639543366