{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Songhu Wang"],"species":["Arabidopsis Thaliana"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0007133000"],"submitter_email":["wangsonghu@ahau.edu.cn"],"submitter_affiliation":["Anhui Agricultural University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Clathrin-mediated vesicular formation and trafficking are responsible for molecular cargo transport and signal transduction among organelles. Our previous study shows that CHLOROPLAST VESICULATION (CV)-containing vesicles (CVVs) are generated from chloroplasts for chloroplast degradation under abiotic stress. Here, we show that CV interacts with the clathrin heavy chain (CHC) and induces vesicle budding toward the cytosol from the chloroplast inner envelope membrane. In the defective mutants of CHC2 and the dynamin-encoding DRP1A, CVV budding and releasing from chloroplast are impeded. The mutations of CHC2 inhibit CV-induced chloroplast degradation and hypersensitivity to water stress. Moreover, CV-CHC2 interaction is impaired by the oxidized GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPC). GAPC1 overexpression suppresses CV-mediated chloroplast degradation and hypersensitivity to water stress, while CV silencing alleviates the hypersensitivity of the gapc1gapc2 plant to water stress. Together, our work identifies a pathway of clathrin-assisted CVV budding outward from chloroplast, which is involved in chloroplast degradation and stress response."],"pubmed_title":["Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by CHLOROPLAST VESICULATION but inhibited by GAPC."],"pubmed_authors":["Pan Ting T, Liu Yangxuan Y, Hu Xufan X, Li Pengwei P, Lin Chengcheng C, Tang Yuying Y, Tang Wei W, Liu Yongsheng Y, Guo Liang L, Kim Chanhong C, Fang Jun J, Lin Honghui H, Wu Zhihua Z, Blumwald Eduardo E, Wang Songhu S"],"additional_accession":[]},"is_claimable":false,"name":"Stress-induced endocytosis from chloroplast inner envelope membrane is mediated by Chloroplast Vesiculation (CV) but inhibited by GAPC","description":"control-1 stands for CPro-CV-GFP control-1  control-2 stands for CPro-CV-GFP control-2 control-3 stands for CPro-CV-GFP control-3 stress -1 stands for CPro-CV-GFP stress -1 stress -2 stands for CPro-CV-GFP stress -2 stress -2 stands for CPro-CV-GFP stress -3 chc2 stress -1 stands for CPro-CV-GFP chc2 stress -1 chc2 stress -2 stands for CPro-CV-GFP chc2 stress -2 chc2 stress -3 stands for CPro-CV-GFP chc2 stress -3","dates":{"publication":"Fri Sep 15 00:00:00 BST 2023"},"accession":"PXD045400","cross_references":{"TAXONOMY":["3702"],"pubmed":["37792531"]}}