iProXProteomicsJianye DaiRattus Norvegicushttp://www.iprox.org/page/project.html?id=IPX0007588000daijy@lzu.edu.cnLanzhou UniversityiProXfalseActivity-based protein profiling reveals Hydroxysafflor yellow A binds to malate dehydrogenase 1 against myocardial ischemia reperfusion injury by maintaining mitochondrial functional homeostasisHydroxysafflor yellow A (HSYA), a natural product derived from Carthamus tinctorius L., has been approved for the treatment of cardiovascular and cerebrovascular diseases and emerged as an effective therapeutic drug on myocardial ischemia reperfusion (MI/R) injury via mechanisms which are poorly understood. We employed Reductive dimethyl labeling with tandem orthogonal proteolysis-activity-based protein profiling (rdTOP-ABPP) method to reveal the direct binding proteins and site in mitochondrion.Sat Nov 18 00:00:00 GMT 2023PXD04708110116