iProXProteomicsChenXi JiaHomo SapiensEscherichia Colihttp://www.iprox.org/page/project.html?id=IPX0008329000cjia99@126.comState Key Laboratory of Medical Proteomics，Beijing Proteome Research Center, National Center for Protein Sciences(Beijing), Beijing Institute of Lifeomics, Beijing 102206, ChinaiProX<i>Escherichia coli</i> Nissle 1917 (EcN 1917) exhibits distinct tumor-targeting activity, and early studies demonstrated that outer membrane vesicles (OMVs) mediate bacteria-host interactions. To decipher the molecular mechanism underlying the interaction between EcN 1917 and host cells via OMV-mediated communication, we investigated the phenotypic changes in Caco-2 cells perturbed by EcN 1917-derived OMVs and constructed proteomic maps of the EcN 1917-derived OMV components and OMV-perturbed host cells. Our findings revealed that the size of the EcN 1917-derived OMV proteome increased 4-fold. Treatment with EcN 1917-derived OMVs altered the proteomic and phosphoproteomic profiles of host cells. Importantly, for the first time, we found that treatment with EcN 1917-derived OMVs inhibited cancer cell migration by suppressing the expression of ANXA9. In addition, phosphoproteomic data suggested that the ErbB pathway may be involved in OMV-mediated cell migration. Taken together, our study provides valuable data for further investigations of OMV-mediated bacteria-host interactions and offers great insights into the underlying mechanism of probiotic-assisted colorectal cancer therapy.Proteomic Analysis of Caco-2 Cells Disrupted by EcN 1917-Derived OMVs Reveals Molecular Information on Bacteria-Mediated Cancer Cell Migration.Zhao Ling L, Zhao Mingxin M, Wang Xiankun X, Jia Chenxi CfalseCellular proteomic perturbed by bacterial OMVs reveals molecular information on bacteria-mediated cancer cell migrationCellular proteomic perturbed by bacterial OMVs reveals molecular information on bacteria-mediated cancer cell migrationWed Mar 06 00:00:00 GMT 2024PXD050386562960638845157