{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Zusen Fan"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0008466000"],"submitter_email":["fanz@moon.ibp.ac.cn"],"submitter_affiliation":["Institute of Biophysics, Chinese Academy of Sciences"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"name_synonyms":["Mus musculus, 3.4.22.-, Caspase-14 subunit p10, Laboratory, Swiss, mice, ANCA-associated vasculitis, Mus domesticus, Swiss Mouse, CASP14, mouse, iecur, mini-ICE, House Mice, Swiss Mice, CG1263, Caspase-14 subunit p19, CASP-14, Rp L8, MICE, House Mouse, rpL8, Laboratory Mice, domesticus, jecur, Livers, DmelCG1263, anon-EST:Posey5, House, Mus, L8, Mini-ICE, CTRL1, Mus musculus domesticus, Mouse, AAV, M(3)62F, Mice, Proteomes, Laboratory Mouse, Mus musculus., antineutrophil cytoplasmic antibody-associated vasculitis, M(3)LS2"],"description_synonyms":["Hac-1/Dark, CDF, Bru, IL1BC, MeCN, Meth, NCMe, Raw, DmelCG6829, CASP-1, CG7788, crice, CREB3L1, protein, Apaf-1, Alkylations, Polypeptides, POF, peptido, CH3-C#N, High Performance Liquid Chromatography, extracellular vesicular exosome, Min, AGAMOUS-like 61, Apaf1, protein aggregate, Il1bc, ARK, DmelCG42273, DiA, (R*, Svc, l(2)k07135, hac-1, peptides, Tissue, hypoplasia, iecur, min, arc, mAPC, Processed cyclic AMP-responsive element-binding protein 3-like protein 1, DIASP, ark, IL-1BC, T1, DIA, Dia, HPLC, AI047805, 4-(4-dihexadecylaminostyryl)N-methylpyridium iodide, High-Performance, column, ICE, Ice, Chromatography, 38E.16, High-Performance Liquid, High Speed Liquid, autolysin activity, necrosis, dApaf1, TEAB, Caspase-1 subunit p10, iCE, ice, D-Apaf-1, drice, anon-53Fa, Liquid Chromatography, Dmel_CG7826, l(2)SH0173, 4-dimercapto-2, Thermo Scientific, DmelCG7788, ms(2)04138, Desmoplastic infantile astrocytoma, Dm1, drIce, drICE, exosome., bacteriocin activity, CG6829, Dmel_CG7835, Crystal, dark/hac-1/dapaf-1, Mnb, MNB, MS/MS, POF2, F27C12_24, P45, F27C12.24, hac1, AW124434, beta Trypsin, Speed, dapaf-1S, R*)-1, apaf-1, lysin activity, dapaf-1L, Acetamide, Drice, DIANA, p45, bacteriolytic toxin activity, Liquid, Polypeptide, methyl cyanide, DRICE, 4-Dimercapto-2, Old astrocyte specifically-induced substance, rac-Dithiothreitol, mouse protein, Dapaf-1/HAC-1, threo-1, DrIce, DrICE, Cleland's reagent, Gene, protein-containing complex, Chalk, CG7826, jecur, Buffer, Dark/Hac-1/dApaf1, Hac1, Dark/Hac-1/dApaf-1, reduced, Oasis, DmelCG1768, Tina, Gene Products, CG7835, HILDA, CG42273, Del(8)44H, tiny, anatomical systems, Glass, MS2, PCE-2, dapaf-1, beta-Trypsin, dapaf, dark, High-Performance Liquid Chromatographies, Solution, Caspase-1 subunit p20, DARK, Ultra Performance Liquid Chromatography, peptidos, UPLC, DIA2, dArk, Dapaf-1, 2-iodo-, High-Performance Liquid Chromatography, small, 3-butanediol, dark/dapaf-1/hac-1, DYRK1, 1-(14)C-labeled, 3H-labeled, caspase 3, protein complex, dApaf-1, Desmoplastic astrocytoma of infancy, Proteins, apaf1, CE-2, CG1768, acetonitrile, DRF2, buffer, Dithiothreitol, Peptide, Dithiotreitol, APAF1, Temperatures, native protein, Hac-1, Protein, MLPLI, DL-threo-1, Dyrk1, CES2A1, Dark, Vacuums, Dias, Dark/Apaf-I, ACETONITRILE, tandem MS, DTL, cyanomethane, HLB, PSEC0238, AU020952, DTT, holin, PRSS, Col4a-1, underdeveloped, CC1, Tripcellim, OASIS, Dark/Dapaf-1/HAC1, IL-1 beta-converting enzyme, ethanenitrile, Interleukin-1 beta-converting enzyme, dApaf-1/DARK/HAC-1, ME-IV, Interleukin-1 beta convertase, Protein Gene Products, cAMP-responsive element-binding protein 3-like protein 1, Livers, Gene Proteins, Trypure, High Pressure, 3.4.22.36, Peptid, High Performance Liquid, dAPAF-1, Ultrasonic"],"additional_accession":[]},"is_claimable":false,"name":"Liver proteomes from WT, Rcrin knockout, AAV-Ctrl and AAV-Rpl8  mice","description":"The liver tissues were disrupted by the ultrasonic processor on ice in lysis buffer. After centrifugation, the extracted proteins were reduced with 10 mM DTT for 2 h at room temperature followed by alkylation with 20 mM iodoacetamide for 30 min in the dark. The protein solution was diluted 1:5 with 50 mM triethylammonium bicarbonate (TEAB) and digested with trypsin (1:50) at 37 °C overnight. The digestion was desalted on OASIS HLB column and peptides eluted with 60% acetonitrile were lyophilized via vacuum centrifugation. All nanoLC-MS/MS experiments were performed on Orbitrap Eclipse (Thermo Scientific) equipped with an Easy n-LC 1200 HPLC system (Thermo Scientific). The DIA raw data from Orbitrap Eclipse were analyzed using Spectronaut version 17 (Biognosys) with the “DirectDIA” mode for protein identification and quantification. The Uniprot mouse protein database (updated on 03-2023) was used for searching the data from exosome samples.","dates":{"publication":"Thu Mar 28 00:00:00 GMT 2024"},"accession":"PXD051039","cross_references":{"TAXONOMY":["10090"]}}