{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Zhenguo Chen"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0008428000"],"submitter_email":["czg1984@smu.edu.cn"],"submitter_affiliation":["Southern Medical University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Centriolar satellites facilitate the delivery of proteins required for centrosome assembly and function, and rely on the nucleation of the scaffold protein pericentriolar material 1 (PCM1). During sperm maturation, centrosomes undergo remodeling to facilitate neckpiece formation and flagellogenesis. However, the role and mechanism of PCM1 in centrosome remodeling and sperm maturation remain to be elucidated. Herein we show that PCM1 is downregulated in patients with non-obstructive azoospermia. Pcm1 knockout mice exhibit disrupted spermiogenesis, characterized by a disorganized manchette and head-tail coupling apparatus, defective flagellogenesis, and male infertility. Mechanistically, PCM1 binds centrosomal proteins and governs their precise translocation via intra-manchette transport, ensuring proper centrosome remodeling and axoneme biogenesis. Inactivation of PCM1 in sperm leads to severe retardation of embryo development, which cannot be overcome by intra-cytoplasmic sperm injection. Collectively, these findings establish PCM1 as a key regulator of spermatogenesis and spermiogenesis, highlighting the contribution of sperm centrosome-related proteins to embryo development and fertility."],"pubmed_title":["PCM1 orchestrates centrosomal and flagellar protein transport to promote sperm maturation."],"pubmed_authors":["Huang Zicong Z, Yi Runduan R, Cen Xixian X, Zhang Hanbin H, Xie Minyu M, Ma Ke K, Ruan Shipeng S, Zhuang Yuge Y, Zhang Xiaoyuan X, Liu Wenyuan W, Feng Hongrui H, Chen Deyu D, Ahmed Mohamed Morsi M MMM, Liu Hong H, Huang Chuyu C, Kang Xiangjin X, Chen Zhenguo Z"],"name_synonyms":["ptc4, Mus musculus, 3.4.22.-, Caspase-14 subunit p10, CXXC3, Peptidomics, Laboratory, Swiss, mice, Mus domesticus, Swiss Mouse, CASP14, mouse, PCM1, C030044G17Rik, mini-ICE, House Mice, Swiss Mice, Caspase-14 subunit p19, CASP-14, MICE, Cxxc3, House Mouse, 9430077F19Rik, Laboratory Mice, domesticus, RFT, pcm-1, 2600002H09Rik, House, Mus, Mini-ICE, PTC4, Mus musculus domesticus, AGM1, Mouse, Mice, Laboratory Mouse, Mus musculus."],"pubmed_abstract_synonyms":["Pericentriolar Material, azoospermia, Postnidation Embryo, Sperm Counts, Male, Pre-implantation Embryo Development, Post-implantation, ciliary axoneme assembly, Low Sperm Count, Cxxc3, Pre implantation Embryo Development, 9430077F19Rik, Embryonic Programming, World Fertility Survey, Fertility Preferences, Embryo Development, Roles, Centrosomal, Below Replacement, symptoms, Concepts, AGM1, Infertility, Injectables, IKKg, Fertility Surveys, multicellular organismal biosynthetic process, male, KEY, Key, Spermatozoa Maturation, Male Infertility, X-Chromosome-Bearing Sperm, Sperm Count, sperm cell, Fertility Incentives, single-organism biosynthetic process, X-Bearing, HTCA, Natural, Absent sperm in semen, Sub-Fertility, Centrosome, Preimplantation Embryo Development, Y Chromosome Bearing Sperm, Maturation, Knock out, \"male infertility\" EXACT [SNOMEDCT_2005_07_31:155924001], Y-Bearing Sperm, X Chromosome Bearing Sperm, infertility, Y-Chromosome-Bearing Sperms, Role Concepts, Fertility Determinant, Centrosomal Associated, Fertility Preference, Pericentriolar Materials, TL, Differential Fertility, ejaculate, Y-Chromosome-Bearing, screening, sterility, Knock-out Mice, embryonal development, aberrant, Marital Fertility., Low Sperm, World Fertility Surveys, PCM1, Knockout Mouse, male reproductive system infertility disorder, RFT, DmIKKgamma, spermatid cell development, Role Concept, single-organism transport, dIKK, Kenny, Role, Region, regulator, X-Chromosome-Bearing, IKK-gamma, signs, infertility disorder of male reproductive system, spermatozoid, X Bearing Sperm, Preferences, Fertility Determinants, seminal fluid, DmelCG16910, \"Infertility, Patient, Material, unspecified\" EXACT [ICD9CM_2006:606.9], Sperms, Knockout, Surveys, Centrosome Proteins, flagellum axoneme assembly, Mouse, Male Sub-Fertility, Fecundability, Postimplantation, Embryonic Programmings, \"male infertility NOS (finding)\" EXACT [SNOMEDCT_2005_07_31:198018002], cilium axoneme assembly, Pericentriolar Matrix, ptc4, atypia, Spermiogenesis, Subfecundity, Sperm, Count, Male Sterility, Matrix, embryogenesis, Gene, Fertility, Development, Y-Chromosome-Bearing Sperm, cilium axoneme biogenesis, dIKK-gamma, Azoospermia (finding), Prenidation Embryo Development, Subfertility, Determinant, spermiogenesis, Injection, DmIKK-gamma, Oligospermia, \"male infertility (finding)\" EXACT [SNOMEDCT_2005_07_31:2904007], Embryonic Developments, Gene Products, Spermatozoon, Survey, Low, atypical, Preference, Animal, dmIKKgamma, World Fertility, IKK[[gamma]], Mice, Y Bearing Sperm, \"male infertility, Fecundity, Knock-out, sperm neck, Postnidation, sperm connecting piece, Postnidation Embryo Development, Oligozoospermia, X-Bearing Sperms, Pre-implantation, X-Chromosome-Bearing Sperms, Injectable, Maturation of Spermatozoa, Male Subfertility, embryogenesis and morphogenesis, IKK, sperm, Clients, male infertility, Below Replacement Fertility, PTC4, SPERM, Fertility Incentive, spermatogenic failure, Pericentriolar Matrices, X-Bearing Sperm, findings, Sub Fertility, World, Proteins, Post implantation Embryo Development, Post-implantation Embryo Development, Preimplantation, C030044G17Rik, Spermatocytogenesis, function, defective, Client, Low Sperm Counts, spermatozoon, Concept, Centrosomal Proteins, IKKgamma, pcm-1, Centrosomes, generation of spermatozoa, male\" EXACT [ICD9CM_2006:606], 2600002H09Rik, Protein, Natural Fertility, Pericentriolar Region, Determinants, Y-Bearing, Postimplantation Embryo Development, Embryonic, Embryo, Counts, Exhibit, male sterility, CXXC3, Dmikkgamma, Pericentriolar, flagellar axoneme assembly, Embryogenesis, axoneme biogenesis, CG16910, implantation fossa, Protein Gene Products, Gene Proteins, Y-Bearing Sperms, Differential, Marital, male reproductive system infertility, Sterility, Pericentriolar Regions, Knockout Mice, Severe, Fertility Survey"],"pubmed_title_synonyms":["Spermatozoa Maturation., ptc4, Sperm, Transmembrane, Gated Protein Transport, Protein Trafficking, CXXC3, enzyme transport, Gated, Protein Translocation, PCM1, C030044G17Rik, Protein Targeting, Traffickings, Cxxc3, 9430077F19Rik, RFT, Maturation of Spermatozoa, Maturation, Cellular Protein Targeting, pcm-1, Vesicular Protein Transport, 2600002H09Rik, Protein, Protein Transport, Transmembrane Protein Transport, PTC4, Vesicular, Protein Sorting, Cellular, AGM1, Protein Localization Processes"],"description_synonyms":["Pericentriolar Matrix, ptc4, Pericentriolar Material, 3.4.22.-, Pericentriolar Matrices, Spermiogenesis, Laboratory, Mus domesticus, CASP14, mouse, PCM1, Matrix, C030044G17Rik, Spermatocytogenesis, mini-ICE, CASP-14, Cxxc3, House Mouse, 9430077F19Rik, RFT, pcm-1, Centrosomes, 2600002H09Rik, House, Mus, Mini-ICE, Mus musculus domesticus, AGM1, Pericentriolar Region, Region, Mice, multicellular organismal biosynthetic process, Mus musculus, single-organism biosynthetic process, Caspase-14 subunit p10, CXXC3, Swiss, mice, Swiss Mouse, Pericentriolar, House Mice, Swiss Mice, Caspase-14 subunit p19, generation of spermatozoa., MICE, Laboratory Mice, domesticus, Material, PTC4, Pericentriolar Regions, Mouse, Pericentriolar Materials, Laboratory Mouse"],"additional_accession":[]},"is_claimable":false,"name":"Testicular Proteomics of PCM1 knockdown mice","description":"Loss of pcm1 in mice disrupts the centrosome remodel and flagellar formation during spermatogenesis.","dates":{"publication":"Wed Apr 03 00:00:00 GMT+01:00 2024"},"accession":"PXD051164","cross_references":{"TAXONOMY":["10090"],"pubmed":["40481240"]}}