iProXProteomicsLiang YangMycobacteroides Abscessus Atcc 19977http://www.iprox.org/page/project.html?id=IPX0008842000yangl@sustech.edu.cnShenzhen Third People's Hospital, the Second Affiliated Hospital of southern University of Science and Technology, National clinical Research center for Infectious DiseaseiProX<i>Pseudomonas aeruginosa</i> and <i>Mycobacterium abscessus</i> are opportunistic pathogens that cause severe infections in hospitals, and their co-infections are increasingly reported. The interspecies interactions between these two bacterial species and their potential impacts on infections are largely unexplored. In this study, we first demonstrated that <i>P. aeruginosa</i> inhibits the growth of <i>M. abscessus</i> by iron chelating via <i>pqs</i> quorum sensing. Next, through proteomic analysis, we discovered that the PQS molecule significantly changed a large amount of protein expression in <i>M. abscessus</i>, including proteins involved in the type VII secretion system and iron homeostasis. Furthermore, we revealed that PQS significantly enhanced the production of bacterial membrane vesicles (MVs) by <i>M. abscessus</i>. Our study suggests that the <i>P. aeruginosa</i> PQS can serve as an interspecies signaling molecule to communicate with <i>Mycobacterium</i> and affect their physiology and virulence.&lt;i&gt;Pseudomonas aeruginosa pqs&lt;/i&gt; Quorum Sensing Mediates Interaction with &lt;i&gt;Mycobacterium abscessus&lt;/i&gt; In Vitro.Long Yun Y, Li Zhi Z, Li Menglu M, Lu Peiyi P, Deng Yujia Y, Wu Pengyao P, Li Xue X, Qin Gangjian G, Huang Jiamin J, Gao Wenying W, Li Guobao G, Jia Tianyuan T, Yang Liang LfalseQuantitative TMT Mass-spec based proteomics on Mycobacterium AbscessusTo study the changes in the TMT proteomics profile of Mycobacterium Abscessus samples, TMT was used as the labeling strategyfor comparative quantitative proteomic analysis.Mon May 20 00:00:00 BST 2024PXD05241956100739858882