{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Qingshan Chen"],"species":["Glycine Max"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0009020000"],"submitter_email":["1261640420@qq.com"],"submitter_affiliation":["Northeast Agricultural University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"name_synonyms":["AI504783, liquid chromatography tandem mass spectroscopy, LC-MS2, CD3epsilon, TCRE, T3E, LCMSMS, CD3E, protein complex, Proteins, LC-MS/MS, Gene, proteins, protein, protein-containing complex, Protein Gene Products, Gene Proteins, protein polypeptide chains, LC/MS/MS, T3e, native protein, natural protein, polypeptide chain, liquid chromatography-tandem mass spectroscopy, CD3e, Protein, LC-MSMS, IMD18, Gene Products, liquid chromatography tandem mass spectrometry, CD3, LC-MS-MS., protein aggregate, T-cell surface antigen T3/Leu-4 epsilon chain"],"description_synonyms":["DCWHKTA, liquid chromatography tandem mass spectroscopy, o-hydroxybenzoate, DUbc9, Endopeptidase Inhibitor, l(3)Ca, LYNAP, Monocyte-derived neutrophil chemotactic factor, determination, classic hairy cell leukaemia, Peptide Hydrolase Inhibitor, d-titin, protein, Hydrogenchlorid, salM, GRO:0005338, Neutrophil-activating factor, IL-8(6-77), prevention, dmTAF[[II]]230, protein polypeptide chains, MDNCF-c, halite, Lymphocyte-derived neutrophil-activating factor, Lwr, hydrogen chloride, Protease Inhibitor, IL-8, ionic compounds, DmelCG1915, 1, Msal-1, 2, Inhibitors, CG6464, Enterococcus coli, protein aggregate, Hydrolase Inhibitors, IL8|NAP1 form III, MDNCF-a, prevention and control, Ket, MDNCF-b, 3-Propanetriol, (Ala-IL-8)77, Oelsuess, LCMSMS, Salm, Escherichia/Shigella coli, enzymes, TFIID TAF250, reference sample, Soybean, cel, N, Soy Beans, Salt, Msal, proteins, sel, Salz, ket, 2-hydroxybenzoic acid ion(1-), IL8|NAP1 form IV, E. coli, salts, cloruro sodico, preventive measures, salt, Wasserstoffchlorid, Peptidohydrolase Inhibitor, MDNCF, SalM, climbing root (narrow), sample, IL8|NAP1 form II, slam, GCP|IL-8 protein V, MONAP, GCP|IL-8 protein I, LC-MS2, Glyceritol, Soybeans, Emoctakin, DmelCG6464, nsal, dTAF[[II]]230, rock salt, preventive therapy, GCP|IL-8 protein III, Peptidase Inhibitor, IL-8(7-77), DPII, DP, titin, LC-MS/MS, TAF200, aerial root (narrow), 0020/01, T-cell chemotactic factor, TAFII-250, classic hairy cell leukemia, TAF250/230, Peptide Peptidohydrolase, Granulocyte chemotactic protein 1, Soy Bean, spalt, TAFII250, common salt, Stickstoff, glycerol, Hydrolase Inhibitor, Chlorwasserstoff, hbl, SPALT, l(2)02858, l(2)05487, l(2)05486, Gro, GCP|IL-8 protein IV, Spalt, glycerine, Antagonists, Phaseolus max, i56, ionic compound, Interleukin-8, HCl, IL8|NAP1 form VI, GCP|IL-8 protein II, root, CG17603, natrii chloridum, TAF[[II]], i105, Neutrophil-activating protein 1, cv. Wye, CG1915, nitrogen, Peptidase Inhibitors, Taf250, SR3-5, liquid chromatography-tandem mass spectroscopy, Biocatalyst, Antiproteases, NAP1, NAF, CG18242, Endopeptidase, Peptide Peptidohydrolase Inhibitor, 7N, chlorane, l(2)03602, Inhibitor, TAF230, CG18245, d230, NAP-1, DmelCG4922, Biocatalysts, chlorure d'hydrogene, Endopeptidase Inhibitors, Gene, Coding, dTAFII250, Protease, protein-containing complex, SLS, Sls, EfW1, LC-MS-MS, Buffer, Glycerine, glycyl alcohol, dip4, Propanetriol, polypeptide chain, dmTAF1, Taf230, Protein 3-10C, Trihydroxypropane, IL-8(1-77), LC-MSMS, Gene Products, Protease Antagonist, Ubc 9, Titin, Peptide Hydrolase, Medical, anon-CREST, IL-8(8-77), cloruro de hidrogeno, DPI, l(3)j1D7, TAF250, Taf200, dTAF[[II]]250, Clinical, GCP|IL-8 protein VI, DmelCG3018, Hydrogen chloride, cell, Medical Coding, soybeans, Glycerin, chlorure de sodium, Taf1p, CG18857, Ubc-9, Antagonist, semi, l(2)01519, soybean, IL-8(5-77), dTAF250, l(3)dre8, Chemokine (C-X-C motif) ligand 8, Enzyme, glycerolum, Protease Antagonists, sels, KZ, Kochsalz, BcDNA:RE40068, Beans, Peptide Peptidohydrolase Inhibitors, l(3)rL182, liquid, IL8, TAF, MCP, Soy, Controlled, CXCL8, Controlling, UBC9, Bacterium coli, TAF[[II]]250, LECT, Ubc9, protein complex, Glyzerin, sales, Proteins, GCP-1, l(3)84Ab, l(3)62Ca, enzyme activity, BG:DS00004.13, chloridohydrogen, B1164, LUCT, Monocyte-derived neutrophil-activating peptide, (Ser-IL-8)72, buffer, GCP1, Cell, Peptide, dTAF230, all, kettin, [HCl], IL8|NAP1 form I, LC/MS/MS, native protein, FBgn0010602, natural protein, Natriumchlorid, IL8|NAP1 form V, Bacillus coli, p230, Protein, dUbc9, chemical analysis, dUBC9, Hydrochloride, sequence, TAF[[II]]250/230, 3602, TFIID, table salt, IL-8(9-77), ubc9, NaCl, CG4922, D-titin, Sal, SAL, Taf[[II]]250, 3-Trihydroxypropane, TAF[[II]]230, Protein., prophylaxis, Bean, B130022O04Rik, Salze, TAF[II]250, azote, D-Titin, Peptidohydrolase Inhibitors, primary structure of sequence macromolecule, sample population, sam, sal, nitrogeno, Protein Gene Products, Gene Proteins, Peptidase, DmelCG17603, C-X-C motif chemokine 8, CT41299, Bacterium coli commune, Antiprotease, control, Dmubc9, Glycine max, l(3)S002001, liquid chromatography tandem mass spectrometry, Peptide Hydrolase Inhibitors, assay, salII, hydrochloric acid, CG3018, DmUbc9, TAF1"],"additional_accession":[]},"is_claimable":false,"name":"Rhizobia T3E NopL interaction protein identified by LC-MSMS","description":"The coding sequence of the NopL was cloned into pET28a vector using BamH I and Sal I restriction enzymes. The resulting vector was transformed into E. coli BL21 (DE3) for the expression of recombinant protein. His-NopL protein was purified by Ni NTA Beads 6FF (Smart-lifesciences, SA005005). DN50 roots inoculated with HH103 were collected at 1 dpi and frozen in liquid nitrogen. Soybean root hair cells were collected by scraping the soybean roots using the cell scraper (Corning Incorporated, 3010). Soybean root hair proteins were extracted using protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10% Glycerol, 0.2% Trixton X-100, 1 mM DTT, 1×protease inhibitor cocktail, 1 mM NaF, 1 mM Na3VO4 and 1 mM PMSF). The obtained His-NopL protein was added to the soybean root protein extract, while no His-NopL protein was added to the control sample. After mixing, the NopL interacting proteins were pull-down using Ni NTA Magarose Beads (Smart-lifesciences, SM008001) semi-in vivo. Finally, the eluted proteins were subjected to analysis using LC-MS/MS to identify and characterize the proteins that interact with NopL.","dates":{"publication":"Sun Jun 09 00:00:00 GMT+01:00 2024"},"accession":"PXD052987","cross_references":{"TAXONOMY":["3847"]}}