iProXProteomicsXiaofeng CaoArabidopsis Thalianahttp://www.iprox.org/page/project.html?id=IPX0009605000xfcao@genetics.ac.cnInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesiProXTranslation, a fundamental process regulating cellular growth and proliferation, relies on functional ribosomes. As sessile organisms, plants have evolved adaptive strategies to maintain a delicate balance between growth and stress response. But the underlying mechanisms, particularly on the translational level, remain less understood. In this study, we revealed the mechanisms of AtPRMT3-RPS2B in orchestrating ribosome assembly and managing translational regulation. Through a forward genetic screen, we identified PDCD2-D1 as a suppressor gene restoring abnormal development and ribosome biogenesis in atprmt3-2 mutants. Our findings confirmed that PDCD2 interacts with AtPRMT3-RPS2B, and facilitates pre-ribosome transport through nuclear pore complex, finally ensuring normal ribosome translation in the cytoplasm. Additionally, the dysfunction of AtPRMT3-RPS2B was found to enhance freezing tolerance. Moreover, we revealed that AtPRMT3-RPS2B promotes the translation of housekeeping mRNAs while concurrently repressing stress-related mRNAs. In summary, our study sheds light on the regulatory roles of AtPRMT3-RPS2B in ribosome assembly and translational balance, enabling the trade-off between growth and stress.AtPRMT3-RPS2B promotes ribosome biogenesis and coordinates growth and cold adaptation trade-off.Wang Zhen Z, Zhang Xiaofan X, Liu Chunyan C, Duncan Susan S, Hang Runlai R, Sun Jing J, Luo Lilan L, Ding Yiliang Y, Cao Xiaofeng XfalseAtPRMT3-RPS2B promotes ribosome biogenesis and coordinates growth and cold adaptation trade-off _DIA MSQuantitative MS analysis for nuclear proteins from Col-0, atprmt3-2, and suppressor m20.Thu Sep 05 00:00:00 GMT+01:00 2024PXD055607370239375381