iProXProteomicsXiangmin LinAeromonas Hydrophilahttp://www.iprox.org/page/project.html?id=IPX0009799000313461278@qq.comFujian Agriculture and Forestry UniversityiProXPost-translational modifications are crucial in regulating biological functions across both prokaryotes and eukaryotes. In Aeromonas hydrophila, CobQ, a recently identified novel deacetylase, plays a significant role in lysine deacetylation, influencing bacterial metabolism and stress responses. The present study utilized quantitative proteomics to investigate the impact of cobQ deletion on the global protein expression profile in A. hydrophila. Through data-independent acquisition mass spectrometry, we identified 233 upregulated and 41 downregulated proteins in the cobQ deletion mutant (ΔahcobQ) strain compared to the wild-type (WT) strain. Key differentially expressed proteins were involved in oxidative phosphorylation, bacterial secretion, and ribosomal function. Additionally, phenotypic assays demonstrated that the ΔahcobQ strain exhibited an increased resistance to oxidative phosphorylation inhibitors, suggesting a pivotal role for AhCobQ in energy metabolism. Outer membrane proteins and efflux pumps also showed altered expression, indicating potential implications for membrane permeability and antibiotic resistance. These results suggested that AhCobQ plays a vital regulatory role in maintaining metabolic homeostasis and responding to environmental stress, highlighting its potential as a target for therapeutic interventions against A. hydrophila infections.Proteomic Insights into the Regulatory Role of CobQ Deacetylase in Aeromonas hydrophila.Wang Guibin G, Chen Linxin L, Lian Juanqi J, Gong Lanqing L, Tian Feng F, Wang Yuqian Y, Lin Xiangmin X, Liu Yanling YCognitive Functions, Cognitive Function, Insights, Cognitions, Proteus ichthyosmius., Functions, Aeromonas dourgesi, Aeromonas hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980), Aeromonas liquefaciens, Function, Proteus hydrophilus, Bacterium hydrophilum, Pseudomonas hydrophila, Insight, Bacillus hydrophilus fuscus, Cognitiveimprinted and ancient gene protein, Gene., study, Peptidomics, protein complex, Proteins, proteins, protein, protein-containing complex, Ximpact, impact-a, Protein Gene Products, Gene Proteins, protein polypeptide chains, native protein, natural protein, polypeptide chain, DEL, Protein, Gene Products, IMPACT, imprinted and ancient gene protein homolog, protein aggregate, E430016J11Rik, RWDD5Proteus hydrophilus, Proteus ichthyosmius., Bacterium hydrophilum, Pseudomonas hydrophila, Bacillus hydrophilus fuscus, Aeromonas dourgesi, Aeromonas hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980), Aeromonas liquefaciensbiochemical pathways, Post Translational Amino Acid Modification, MGC130048, Metabolic Process, Resistances, Product, Lysine Hydrochloride, Metabolisms, Metabolic Concepts, A4, Infestations and Infections, Bioenergetics, protein, Protein Processing, Mendosicutes, Drug Antimicrobial Resistance, Drug Resistances, protein polypeptide chains, Lysine Acetate, Roles, Oxidative, Biological, Aeromonas liquefaciens, efflux, Concepts, 2, Bacterium hydrophilum, deacetylation, Analysis, Biological Product, Metabolism Concept, Expenditure, protein aggregate, Phenomenon, IKKg, L Lysine, KEY, Key, imprinted and ancient gene protein, Mass Spectrum Analysis, Post-Translational Protein, treatment, gamma sarcoglycan, Biologic Drugs, Metabacteria, Analyses, Post-Translational, K, Natural, catabolism, epsilon-diaminocaproic acid, Bodily, Pseudomonas hydrophila, metabolic process resulting in cell growth, proteins, Biological Drugs, Eucarya, inhibition of homeostatic process, Posttranslational Protein Processing, ecotype, DIA, eucaryotes, Bacteria Woese et al. 2024, Secretion, Biological Medicine, LYS, Prokaryotae, Lysin, Secretions, Role Concepts, disease management, Medicine, Drug Antimicrobial Resistances, Therapies, gamma-sarcoglycan, homeostasis, biotransformation, Medicines, Procaryotae, Strains, Biologic Drug, Catabolism, Therapy, Post-Translational Modification, Archaebacteria, Biologic Products, Process, external secretion, metabolism resulting in cell growth, Modifications, SG-gamma, Bacillus hydrophilus fuscus, Microbial, Spectrum Analysis, Spectroscopy, exocrine gland fluid/secretion, Bodily Secretion, DmIKKgamma, Post Translational Modifications, Role Concept, positive regulation of homeostatic process, Aeromonas dourgesi, Playthings and Play, dIKK, Biopharmaceuticals, Kenny, Role, lysine, Plaything, Biologic Product, prokaryotes, secretion, sarcoglycan, cultivar, Phosphorylation, environmental stress, Biological Medicines, Biological Drug, Processing, IKK-gamma, Eukarya, Toys, Spectrometry, Proteus hydrophilus, Infection and Infestation, Biologics, alpha, gamma (35kDa dystrophin-associated glycoprotein), Treatments, Energy Metabolisms, DMDA, DmelCG16910, Amino Acid Modification, antagonists and inhibitors, Antimicrobial Drug Resistance, Aeromonas hydrophila (Chester 1901) Stanier 1943 (Approved Lists 1980), 35kD dystrophin-associated glycoprotein, Posttranslational Modifications, Antimicrobial Resistance, E430016J11Rik, Strains and Sprains, exocrine gland secretion, Posttranslational Modification, Prokaryota, Plays, Post-Translational Protein Modifications, SGCG_HUMAN, exocrine gland fluid, Biologic Pharmaceuticals, Peptidomics, exocrine gland fluid or secretion, Processes, Drug Antimicrobial, Eukaryotes, Gene, eubacteria, Spectrum Analyses, Metabolic Processes, protein-containing complex, Ximpact, TYPE, dIKK-gamma, Posttranslational, DAGA4, Antimicrobial Drug Resistances, polypeptide chain, Antibiotic Resistance, Metabolism, DmIKK-gamma, 35DAG, Post Translational Modification, Gene Products, Mass, Antibiotic, Post-Translational Modifications, Lysine, Bacteriobiota, dmIKKgamma, MAM, gamma-SG, IKK[[gamma]], Metabolism Phenomena, Sprains, SCG3, Post Translational, Mass Spectroscopy, permeability of membrane, Biologicals, Proteus ichthyosmius, Toy, Drugs, Modification, study, Playthings, Natural Product, inhibiteur, Metabolic Concept, Energy, Infections and Infestations., Expenditures, respiratory-chain phosphorylation, Monera, Protein Modifications, Post-Translational Amino Acid Modification, Bacteria (ex Cavalier-Smith 1987), secreted substance, Infestation and Infection, Oxidative Phosphorylations, Puppets, inhibidor, IKK, bodily secretion, Posttranslational Amino Acid Modification, negative regulation of homeostatic process, Posttranslational Protein, Play, Archaebiota, Biologic, Puppet, Pharmaceuticals, Autoregulation, inhibitors, Products, bacteria, Eukaryote, degradation, 35 kDa dystrophin-associated glycoprotein, Biologic Medicines, protein complex, Proteins, Sprain, Phosphorylations, Antimicrobial Resistances, Post-Translational Protein Modification, inhibitor, Eukaryotas, function, impact-a, SGCG, LGMD2C, eukaryotes, Concept, Metabolic Phenomena, strain, Metabolism Concepts, IKKgamma, MS, native protein, natural protein, Acetate, Protein, Biopharmaceutical, Phenomena, Strain, Resistance, Infection, IMPACT, imprinted and ancient gene protein homolog, EnvironmentalStress, Protein Modification, regulation of homeostatic process, L-Lysine, metabolism, Mass Spectrum Analyses, Metabolic Phenomenon, Energy Expenditure, antagonists, Mass Spectrum, multicellular organism metabolic process, biodegradation, DMDA1, Metabolic, Dmikkgamma, Post Translational Protein Processing, not Bacteria Haeckel 1894, archaea, Energy Expenditures, CG16910, Bioenergetic, Drug, Protein Gene Products, Gene Proteins, Eukaryotae, membrane permeability trait, Post Translational Protein Modification, activation of homeostatic process, Therapeutic, DEL, prokaryote, SCARMD2, Post-Translational Protein Processing, Natural Products, Treatment, Eucaryotae, 6-diaminohexanoic acid, Enisyl, RWDD5, AnabolismfalseProteomic Insights into the Regulatory Role of CobQ Deacetylase in Aeromonas hydrophilaThis study utilizes quantitative proteomics to investigate the impact of the cobQ deletion (ΔahcobQ) on the global protein expression profile in A. hydrophila.Tue Sep 24 00:00:00 BST 2024PXD05620764439659247