{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Xiaojun Wang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0009750000"],"submitter_email":["wangxiaojun@caas.cn"],"submitter_affiliation":["Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Human ANP32A/B (huANP32A/B) poorly support the polymerase activity of avian influenza viruses (AIVs), thereby limiting interspecies transmission of AIVs from birds to humans. The SUMO-interacting motif (SIM) within NS2 promotes the adaptation of AIV polymerase to huANP32A/B via a yet undisclosed mechanism. Here we show that huANP32A/B are SUMOylated by the E3 SUMO ligase PIAS2α, and deSUMOylated by SENP1. SUMO modification of huANP32A/B results in the recruitment of NS2, thereby facilitating huANP32A/B-supported AIV polymerase activity. Such a SUMO-dependent recruitment of NS2 is mediated by its association with huANP32A/B via the SIM-SUMO interaction module, where K68/K153-SUMO in huANP32A or K68/K116-SUMO in huANP32B interacts with the NS2-SIM. The SIM-SUMO-mediated interactions between NS2 and huANP32A/B function to promote AIV polymerase activity by positively regulating AIV vRNP-huANP32A/B interactions and AIV vRNP assembly. Our study offers insights into the mechanism of NS2-SIM in facilitating AIVs adaptation to mammals."],"pubmed_title":["Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction."],"pubmed_authors":["Sun Liuke L, Guo Xing X, Yu Mengmeng M, Wang Xue-Feng XF, Ren Huiling H, Wang Xiaojun X"],"additional_accession":[]},"is_claimable":false,"name":"Mass spectrometry analysis of Flag-H9N2-NS2 interacting proteins","description":"Mass spectrometry analysis of Flag-H9N2-NS2 interacting proteins","dates":{"publication":"Fri Sep 27 00:00:00 GMT+01:00 2024"},"accession":"PXD056322","cross_references":{"TAXONOMY":["9606"],"pubmed":["39737943"]}}