{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Jigang Wang"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0010157000"],"submitter_email":["jgwang@icmm.ac.cn"],"submitter_affiliation":["Southern University of Science and Technology"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Rheumatoid arthritis (RA), a prevalent and incurable autoimmune disease globally, is characterized by the immune system attacking the body's own tissues, leading to joint inflammation and damage. Capsaicin (CAP), from <i>Capsicum annuum L</i>., is known for its burning sensation-inducing property and has shown various pharmacological effects, yet its specific mechanisms and targets in RA treatment remain largely unclear. This study aimed to investigate the role of CAP in RA by synthesizing CAP probes and using activity-based protein profiling. We found that CAP reduced joint swelling in arthritic mice and exerted anti-inflammatory and antiproliferative effects on fibroblast-like synoviocytes. We identified that CAP binds to PRDX2, inhibiting its antioxidant function and inducing oxidative stress and apoptosis, contributing to the antiarthritic effects. These results suggest that PRDX2 is a potential target for CAP in RA treatment, providing new insights into the molecular mechanisms and potential therapeutic strategies for RA."],"pubmed_title":["Inhibition Peroxiredoxin-2 by Capsaicin Ameliorates Rheumatoid Arthritis via ROS-Mediated Apoptosis in Fibroblast-Like Synoviocytes."],"pubmed_authors":["He Hengkai H, Hao Mingjing M, Luo Piao P, Chen Junhui J, An Yehai Y, Huang Jingnan J, He Ruiyi R, Du Qingfeng Q, Zhang Qian Q, Wang Jigang J"],"additional_accession":[]},"is_claimable":false,"name":"capsaicin ameliorates rheumatoid arthritis via ROS-mediated apoptosis in fibroblast-like synoviocytes","description":"In this project we discovered that CAP not only reduces joint swelling in arthritic model mice but also exhibits anti-inflammatory and antiproliferative effects on fibroblast-like synoviocytes (FLS). By synthesizing alkyne-tagged CAP probes and employing activity-based protein profiling (ABPP), we identified and confirmed that CAP binds to PRDX2, inhibiting its antioxidant function and thereby inducing cellular oxidative stress and apoptosis, which contribute to its anti-arthritic effects. Furthermore, we found that the PRDX2 inhibitor, Conoidin A (ConA), produces similar effects. The results indicate that PRDX2 serve as a potential target for capsaicin to therapeutic intervention in rheumatoid arthritis.","dates":{"publication":"Thu Nov 07 00:00:00 GMT 2024"},"accession":"PXD057639","cross_references":{"TAXONOMY":["9606"],"pubmed":["40443720"]}}