{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Ying Huang"],"species":["Drosophila Melanogaster"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0010502000"],"submitter_email":["huangy@shsmu.edu.cn"],"submitter_affiliation":["Xinhua Hospital Affiliated to Shanghai Jiao Tong University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Drosophila P75 (dP75), a homolog of the human LEDGF/p75, is crucial for oogenesis by recruiting the histone kinase Jil-1 to euchromatin and impeding H3K9me2 spreading. Like LEDGF, dP75 binds transcriptionally active chromatin, but its precise mechanism remains unclear. Here we show that its PWWP domain prefers binding to thymidine-rich DNA over GC-rich sequences. Crystal structures both in apo and ssDNA-bound states, reveal a domain-swapped homodimer. The aromatic cage, known to recognize histone methyllysine, also engages thymine. Mutations in this cage mimic dP75 knockout phenotypes, including impaired chromatin binding, transposon upregulation, and female sterility. Although dP75 maintains chromatin-bound in H3K36A mutant flies, alterations in the aromatic cage disrupt this localization, underscoring its role in DNA binding. These findings reveal how dP75 targets euchromatin through a PWWP domain that integrates histone reading and nucleotide recognition, advancing our understanding of PWWP domains."],"pubmed_title":["Structural basis of thymidine-rich DNA recognition by Drosophila P75 PWWP domain."],"pubmed_authors":["Jin Zhaohui Z, Meng Zhe Z, Liu Yanchao Y, Li Chongyang C, Zhang Xuedi X, Yin Yue Y, Gao Guanjun G, Dou Kun K, Huang Ying Y"],"name_synonyms":["subgenus>, p75NTR, fruit fly, Drosophila Fallen, GOLIM6, p52, Nbla10317, CDP, Clox, Tumor necrosis factor receptor superfamily member 1b, CDP1, DmelCG9373, IL15RB, TNFR-II, CASP, TNF-RII, hrp59, CUX, p75NGFR, dSRp54, anon-EST:Liang-2.16, High affinity IL-2 receptor subunit beta, LckBP1, Flies, CG4602, Cux/CDP, TNF-R2, LEDGF, clone 2.16, Drosophilas, p75, Fly, IL-2R subunit beta, CDP|Cut, Drosophila Fruit, TNFalpha-R2, Tumor necrosis factor-binding protein 2, cg9373, p200, DFS70, fruit flies, Hematopoietic cell-specific LYN substrate 1, TBP-2, COY1, TNF-R-II, KSRP, Cux|CDP, PSIP2, Hrp59, p80 TNF-alpha receptor, HS1, DmelCG4602, CG9373, p110, membrane form, Etanercept, genus>, TNFBR, hnRNP M, Tnfr-2, Tnfr-1, TNF-alphaR2, FUSE-binding protein 2, TNFRII, Tumor necrosis factor receptor 2, p100, p75TNFR, TNFR1B, RNNGFRR, 1823, lckBP1, LNGFR, Drosophila , Tnfrsf16, fruit fly , high affinity IL-2 receptor subunit beta, CTTNL, CD122., Tumor necrosis factor receptor type II, CD120b, p70-75, Fruit Flies, Tnfr2, CUTL1, Drosophila, TNFR2, SRp54, CDP/Cut, Drosophila Fruit Fly, IL-2RB, KH type-splicing regulatory protein, Fruit Fly, IL2RB, IL-2 receptor subunit beta, TNF-R75, TBPII, PAIP, TNFR80, Drosophila , Euchromatins, fruit fly, Meth, postpartum sterility, Nbla10317, CDP, Clox, Tumor necrosis factor receptor superfamily member 1b, sci, CDP1, IL15RB, Mutations, mAb2A, Histone H2b, 5-Methyluracil, TNF-RII, Histone H2a, Readability, hrp59, IL-1, CUX, lamin/chromatin binding, 4-dihydroxy-5-methylpyrimidine, Roles, CG9063, symptoms, Concepts, HOW, How, 2, Kinase, establishment and maintenance of substrate location, LckBP1, Flies, Chromatins, l(3)j5D5, CG4602, Cux/CDP, Female Infertility, 24B, Su(var)3-103, LEDGF, thymus nucleic acid, Man (Taxonomy), 5-methyl-2, Drosophilas, Fly, stru, Ledgf, T, l(3)S053606, Female, BcDNA:GH03694, CG10293, Sub-Fertility, CDP|Cut, 5 Methyluracil, l(3)j5B5, Thy, female subfertility, Ice, CT26, 3H)-Pyrimidinedione, chromosome scaffold, Role Concepts, Histone H5, Histone H4, Histone H7, Double-Stranded DNA, Psip2, fruit flies, deoxyribonucleic acids, DNAn, Nucleotide, Hematopoietic cell-specific LYN substrate 1, TBP-2, COY1, DmelCG9063, Histone H1, Histone H3, homodimer, TNF-R-II, nuclear chromatin, establishment and maintenance of substance location, screening, sterility, Thymin, 0904/17, p80 TNF-alpha receptor, HS1, DmelCG4602, Modern, Up-Regulation (Physiology), Etanercept, TNFBR, Double-Stranded, postpartum, Su-var(3)103, female, hnRNP M, Tnfr-2, Tnfr-1, (Deoxyribonucleotide)n+m, TNF-alphaR2, single organism localization, FUSE-binding protein 2, Role Concept, SZ1, 2'-Deoxythymidine, Role, p75TNFR, TNFR1B, RNNGFRR, desoxyribose nucleic acid, transcriptionally active chromatin, Female Sterility, nucleotides, 1823, lckBP1, Crystal, LNGFR, Drosophila , Tnfrsf16, JIL1, True Flies, Jil1, Upregulation, BcDNAGH03694, SBP75, fruit fly , signs, CTTNL, 5-methyluracil, 3H)-pyrimidinedione, Tumor necrosis factor receptor type II, human, 3L6, Tnfr2, CUTL1, Speed, Phenotypes, Drosophila, Female Subfertility, TNFR2, SRp54, rich, CDP/Cut, JIL, Drosophila Fruit Fly, IL-2RB, ds DNA, KH type-splicing regulatory protein, anon-EST:Liang-2.39, DNA, IL-2 receptor subunit beta, TNF-R75, TBPII, PAIP, establishment and maintenance of cellular component location, Drosophila , CAGE (cap analysis gene expression), qkr, l(3)S090417, Histone, nuclear membrane vesicle binding to chromatin, Concept, CAGE, TNFRII, KH93F, Tumor necrosis factor receptor 2, structure specific DNA binding, p100, sub-fertility, 4(1H, establishment and maintenance of localization, ds-DNA, ALS1, DmelCG6297, 2' Deoxythymidine, who, hSod1, Oogeneses, high affinity IL-2 receptor subunit beta, single-organism localization, Who/How, Histone H1(s), CD120b, p70-75, Fruit Flies, 5-methyl-, localisation, HEL-S-44, Modern Man, Sterility, Desoxyribonukleinsaeure, qkr[93F], Fruit Fly, Protamine, female sterility, IL2RB, female sub-fertility, TNFR80, CD122"],"pubmed_title_synonyms":["subgenus>, p75NTR, fruit fly, Drosophila Fallen, DNS, (Deoxyribonucleotide)n, GOLIM6, p52, Nbla10317, CDP, Clox, Tumor necrosis factor receptor superfamily member 1b, CDP1, Deoxyribonucleic acids, DmelCG9373, Deoxythymidine, IL15RB, TNFR-II, CASP, TNF-RII, hrp59, CUX, Deoxyribonucleic Acid, CG9063, p75NGFR, dSRp54, anon-EST:Liang-2.16, High affinity IL-2 receptor subunit beta, LckBP1, Flies, CG4602, Cux/CDP, TNF-R2, thymus nucleic acid, LEDGF, clone 2.16, Drosophilas, p75, Fly, Double Stranded, Deoxyribonucleic acid, IL-2R subunit beta, BcDNA:GH03694, CDP|Cut, Drosophila Fruit, TNFalpha-R2, Tumor necrosis factor-binding protein 2, cg9373, p200, Double-Stranded DNA, (Deoxyribonucleotide)m, DFS70, deoxyribonucleic acids, fruit flies, DNAn, Hematopoietic cell-specific LYN substrate 1, TBP-2, COY1, DmelCG9063, TNF-R-II, KSRP, Cux|CDP, PSIP2, Hrp59, p80 TNF-alpha receptor, HS1, DmelCG4602, CG9373, DNAn+1, p110, membrane form, Etanercept, genus>, Double-Stranded, TNFBR, hnRNP M, Tnfr-2, Tnfr-1, (Deoxyribonucleotide)n+m, TNF-alphaR2, FUSE-binding protein 2, TNFRII, 2'-Deoxythymidine, Tumor necrosis factor receptor 2, p100, p75TNFR, TNFR1B, RNNGFRR, ds-DNA, desoxyribose nucleic acid, 2' Deoxythymidine, 1823, lckBP1, LNGFR, Drosophila , Tnfrsf16, BcDNAGH03694, fruit fly , high affinity IL-2 receptor subunit beta, CTTNL, CD122., Tumor necrosis factor receptor type II, 3L6, CD120b, p70-75, Fruit Flies, Tnfr2, CUTL1, Drosophila, TNFR2, SRp54, rich, CDP/Cut, Drosophila Fruit Fly, IL-2RB, ds DNA, Desoxyribonukleinsaeure, KH type-splicing regulatory protein, Fruit Fly, DNA, IL2RB, IL-2 receptor subunit beta, TNF-R75, TBPII, PAIP, TNFR80, Drosophila