iProXProteomicsXiaozhong PengHomo Sapienshttp://www.iprox.org/page/project.html?id=IPX0010843000pengxiaozhong@pumc.edu.cnInstitute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical CollegeiProXfalseIdentifying the target of compound 10 via DARTS assayH1299 cells were collected and lysed using M-PER lysis reagent (Thermo, 78501), and the cell lysates were incubated with compound 10 or DMSO (100 μM) for 1 h at room temperature. The lysates were equally divided and degraded with a 1:30 dilution of protease (Sigma, 10165921001, 1.25 mg/ml) for 30 min. Treated protein samples were sent to DARTS detection.Mon Jan 13 00:00:00 GMT 2025PXD0596949606