{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Xiuling Li"],"species":["Homo Sapiens","Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0009561000"],"submitter_email":["lixiuling@dicp.ac.cn"],"submitter_affiliation":["Dalian Institute of Chemical Physics"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Serial multi-omic analysis of proteome, phosphoproteome, and glycoproteome is pivotal for elucidating drug mechanisms, discovering biomarkers, and identifying therapeutic targets. However, simultaneous multi-level post-translational modifications (PTMs) analysis via parallel processing is hampered by laborious, time-consuming procedures and inconsistent reproducibility. We present an integrated Multi-level PTMs-Proteomic Enrichment platform (MuPPE), enabling sequential glycoproteome, phosphoproteome, and proteome analysis from single biological samples. It combines protein aggregation capture with on-bead digestion and tandem enrichment, achieving superior reproducibility (CV 12.3% vs 17.6% conventional methods) while reducing processing time by 87.5% (4 hours vs 32 hours). MuPPE also enhances coverage, identifying more serum glycopeptides and brain phosphopeptides than other platforms. Applied to aging mouse cohorts, the platform uncovers tissue-specific PTMs remodeling and brain barrier dysfunction. For arsenic mechanisms of action, MuPPE reveals drug-induced PTMs crosstalk between glycosylation and phosphorylation-driven pathway regulation. MuPPE offers a transformative tool for advancing multi-omics insights across precision medicine and disease research."],"pubmed_title":["A versatile platform for sequential glyco-, phospho-, and proteomics with multi-PTMs integration."],"pubmed_authors":["Dong Xuefang X, Xiong Fangfang F, Du Guangzhu G, Yang Yunfei Y, Chen Cheng C, Cui Yun Y, Ding Xinlian X, Li Xiuling X, Shen Yidong Y, Liang Xinmiao X"],"additional_accession":[]},"is_claimable":false,"name":"Mass spectrometry data for proteomics and PTM proteomics","description":"Mass spectrometry data for proteomics and PTM proteomics","dates":{"publication":"Sat Jan 11 00:00:00 GMT 2025"},"accession":"PXD059701","cross_references":{"TAXONOMY":["10090","9606"],"pubmed":["41605919"]}}