{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Xiaoping Wang"],"species":["Colaphellus Bowringi"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0010971000"],"submitter_email":["xpwang@mail.hzau.edu.cn"],"submitter_affiliation":["Huazhong Agricultural University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes. This process is characterized by arrested ovarian development and lipid accumulation in females. A reduction in juvenile hormone (JH) biosynthesis is known to initiate reproductive diapause, but its regulatory mechanism remains unclear. Seven up (Svp), a transcription factor from the nuclear receptor family, plays a crucial role in various developmental processes in insects. In this study, using the cabbage beetle Colaphellus bowringi as a model, we observed higher expression of Svp in the heads of female adults under reproductive photoperiodic conditions (short-day [SD]) compared to diapause conditions (long-day [LD]). RNA interference-mediated knockdown of Svp in SD females induced typical diapause phenotypes, including ovarian arrest and lipid accumulation. The application of methoprene (ME), a JH receptor agonist, reversed these diapause phenotypes and restored reproduction, indicating that Svp's regulation of reproductive diapause is dependent on JH signaling. Additionally, Svp knockdown led to the downregulation of JH pathway genes and a reduction in JH titers. Further evidence suggested that Svp regulates the expression of JHAMT1, a critical gene in JH biosynthesis, which determines diapause entry in C. bowringi. These findings suggest that diapause-inducing photoperiods suppress Svp expression, blocking JH production and triggering diapause. This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production, providing a potential target for controlling pests capable of entering reproductive diapause.","In insects, the juvenile hormone (JH) biosynthetic pathway regulates the in vivo JH titer. Thus, its downregulation potentially contributes to the lowering of JH titers typically observed in insects undergoing reproductive diapause, a developmental arrest at the adult stage. However, no systematic evidence has yet been presented to demonstrate the physiological and genetic roles of JH biosynthetic genes in reproductive diapause. In this work, we performed RNA interference (RNAi)-based reverse genetic analyses by targeting JH biosynthetic genes, followed by analysis of the reproductive diapause traits in Colaphellus bowringi, an economically important cabbage beetle. We identified a total of 22 genes encoding homologues of enzymes involved in the mevalonate pathway and the JH branch of JH biosynthesis in C. bowringi. Among these, 18 genes showed significant downregulation of their expression in the long day-induced diapausing females, compared to the short day-induced reproductive females. RNAi knockdown of almost any one of the 18 genes in reproductive females reduced the expression of the JH-responsive gene, Krüppel homolog1 (Kr-h1), indicating a lowered circulating JH. Most importantly, depleting transcripts of 3-hydroxy-3-methylglutaryl-CoA reductase 2 (HMGR2), farnesyl-pyrophosphate synthase 1 (FPPS1) and juvenile hormone acid methyltransferase 1 (JHAMT1) induced diapause-associated traits, including immature and inactive ovaries, large accumulations of lipids and adult burrowing behavior. Meanwhile, genes related to ovarian development, lipid accumulation and stress response showed expression patterns like those of diapausing females. RNAi-mediated diapause phenotypes could be reversed to reproductive phenotypes by application of methoprene, a JH receptor agonist. These results suggest that photoperiodic reproductive diapause in C. bowringi is triggered by transcriptional suppression of JH biosynthetic genes, with HMGR2, FPPS1 and JHAMT1 playing a critical role in this process. This work provides sufficient evidence to reveal the physiological roles of JH biosynthetic genes in reproductive diapause."],"pubmed_title":["Seven up regulates reproductive diapause initiation via juvenile hormone biosynthesis in the cabbage beetle Colaphellus bowringi.","Juvenile hormone biosynthetic genes are critical for regulating reproductive diapause in the cabbage beetle."],"pubmed_authors":["Tian Zhong Z, Guo Shuang S, Li Jia-Xu JX, Zhu Fen F, Liu Wen W, Wang Xiao-Ping XP","Wang Kou K, Zhao Yu-Lian YL, Jiang Yan-Zi YZ, Liu Wen W, Wang Xiao-Ping XP"],"name_synonyms":["Mass Spectrum Analysis, Spectroscopy, Mass Spectrum, MS, Analyses, Mass, head (volume), Spectrometry, cephalic area., Analysis, Heads, Spectrum Analyses, adult head, Mass Spectrum Analyses, Spectrum Analysis, Mass Spectroscopy"],"pubmed_abstract_synonyms":["Networks, T-cell leukemia, Materials, SVP-1, Kinship, Nuclear Hormone, AGL4, Receptors, AGL22, dormancy, Human Reproductive Indexes, ZR 515, Nuclear Receptor, Light-Dark Cycle, Dark-Light Cycles, Downregulation, Social Controls, E)-, Roles, Nuclear Hormone Receptors, insects, Atelocerata, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, Cytoplasmic Receptor, Post Transcriptional, symptoms, Concepts, Life Cycle, 2, 7, Reproductive Periods, ovary development, Microeconomic, Formal Social Controls, adult, multicellular organismal biosynthetic process, Family Life Cycle, 11-trimethyl-2, Human Reproductive Index, single-organism biosynthetic process, Theory, Utility Theories, Kinship Network, reference sample, Hormone Receptor, Household Consumption, Svp, SVP, anabolism, Economic Policies, F10N7_150, Heads, Living Costs, Cauliflower, quelling, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Cabbage, Altosid, Social, Insect Growth Regulator, Gene Silencings, NR2F3, Cabbages, Light Dark Cycle, Role Concepts, reproductive physiological process, l(3)ck16, AGAMOUS-like 4, Periods, Household Consumptions, Economic, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, Theories, Juvenile Hormone, Family Life Cycles, Cytoplasmic Receptors, E)-11-methoxy-3, TRANSCRIPTION FACTOR, single organism signaling, RNA polymerase II distal enhancer sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific binding, screening, anatomical protrusion, Home Economics, Indices, Index, Hormones, 4-dodecadienoate, Hypothesis, Light, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, isopropyl (EE)-(RS)-11-methoxy-3, female, Macroeconomic Factors, Economics, Reproductive Index, ck16, Nuclear Hormone Receptor, Altosid PS 10, Nuclear and Cytoplasmic Receptors, Economic Condition, Role Concept, Household, Receptor Down-Regulation, Playthings and Play, agonist, Utility Theory, Human Reproductive Indices, Lipid, lethargus., Role, Plaything, AT2G22550, Broccoli, Genetic Materials, Daylight, Uniramia, Macroeconomic, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, diapause, Filiation, RNA Silencing, Adults, Genetic Material, Post-Transcriptional Gene, CG11502, F14P3.4, Light Cycle, Factors, Hormone Receptors, death rate, Consumer Price Indices, F14P3_4, Toys, Control, signs, hexapods, 0598/01, INSDC_feature:gene, Controls, Co Suppression, Transcription factor, Home, Post Transcriptional Gene Silencing, Condition, Life Cycles, Phenotypes, Cytoplasmic and Nuclear Receptors, Dark-Light, Gene Silencing, Easterlin Hypothesis, 0106/18, spine, Material, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, l(3)rA028, Cistron, Intracellular Membrane, Co-Suppression, metal ion regulated sequence-specific DNA binding, Regulation, Intracellular, metal ion regulated proximal promoter sequence-specific DNA binding, Remittances, Regulations, lipids, post-transcriptional gene silencing by RNA, Policies, 0351/01, Membrane Receptors, Plays, bioformation, Family Member, l(3)rL069, 4-Dodecadienoic acid, Utility, Growth Regulator, Gene, Growth Regulators, Beetle, Cytosolic and Nuclear Receptors, Network, SEPALLATA 2, 0861/07, SHORT VEGETATIVE PHASE, Transcription Factor, (E, CG18158, Human, protrusion, Hormone, Intracellular Membrane Receptors, Easterlin, 1-methylethyl ester, Nuclear and Cytoplasmic, zinc ion regulated core promoter proximal region sequence-specific DNA binding, ZR-515, Cycle, 0042/01, Light Cycles, Toy, Cycles, don, study, Transcription, Genetic, 4E)-11-methoxy-3, Playthings, Research, Kale, BcDNA:GH08189, l(3)j2E2, Microeconomic Factors, Economic Conditions, Cosuppression, Reproductive Indices, F14M13.6, l(3)07842, Cytoplasmic, Puppets, Daylight Cycles, l(3)87Bd, E)-isopropyl 11-methoxy-3, RNA polymerase II proximal promoter sequence-specific DNA binding, time of survival, Capital, copper ion regulated proximal promoter sequence-specific DNA binding, 4-dodecadienoic acid 1-methylethyl ester, F14M13_6, Play, isopropyl (2E, Kinship Networks, Family, Human Reproductive, Puppet, Controlled, Policy, agonista, Light-Dark, Economic Policy, Reproductive, Regulators, RNA, Controlling, findings, Cytoplasmic Hormone, Family Research, ovarian development, female human body, Formal Social Control, Conditions, Reproductive Period, posttranscriptional gene silencing by siRNA, Brussel Sprout, 11-methoxy-3, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, Production, Collard Greens, cosuppression, Living Cost, Cistrons, 11-trimethyldodeca-2, Down Regulation, Juvenile, Concept, Consumption, Insect Growth Regulators, transcription factor activity, Family Members, RNAi, E)-1-Methylethyl 11-methoxy-3, SVP1, Down-Regulation, survival, Interference, Period, Consumer Price Index, Social Control, Consumer Price, Down-Regulation (Physiology), Insect Growth, AGAMOUS-like 22, RNA polymerase II transcription factor activity, Cytoplasmic Hormone Receptors, agonists, Insects, Nuclear, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, Light-Dark Cycles, Daylight Cycle, 0005/12, KNOTTED1-like homeobox gene 5, Silencing, agoniste, Cytosol and Nuclear, Post-Transcriptional Gene Silencings, Regulator, Posttranscriptional, Posttranscriptional Gene Silencing, DmelCG11502, Altosid PS10, metal ion regulated core promoter proximal region sequence-specific binding, 0114/20, copper ion regulated core promoter proximal region sequence-specific binding, 4-dienoate, Insect, F10N7.150, Nuclear Receptors, Dark-Light Cycle, Post-Transcriptional, 11-trimethyl-, signalling process, Remittance, Cytosolic and Nuclear, lethargus, Families, Indexes, Cost of Living, Collard Green, Dark Light Cycle, Cytosol and Nuclear Receptors, Altosid PS-10, Beetles, regulation, Photoperiods, Receptor, Post-Transcriptional Gene Silencing, Relatives, ZR515, Females, Tracheata"],"pubmed_title_synonyms":["juvenile hormone biosynthesis, juvenile hormone anabolism, juvenile hormone synthesis, Cabbages, lethargus, Kale, Collard Green, Broccoli, Beetles, Brussel Sprout, dormancy, juvenile hormone formation, diapause, Cauliflower, Collard Greens, Beetle., Cabbage"],"description_synonyms":["Co-Immunoprecipitations, study, protein complex, Co-Immunoprecipitation, gel, Proteins, Precipitation, Gene, Beetle, protein, protein-containing complex, CG1106, Co Immunoprecipitation, Protein Gene Products, Gene Proteins, Immune, native protein, DmelCG1106, Protein, Immune Precipitations, Gene Products, DGS, Beetles, Precipitations, protein aggregate, Beetle., Immune Precipitation"],"additional_accession":[]},"is_claimable":false,"name":"Mass spectrometry identification of immunoprecipitated head samples of Colaphellus bowringi","description":"We used immunoprecipitation to purify total proteins from the beetle using anti-JHAMT1. Subsequently, protein identification was carried out after excising the target area from the gel. This study aims to identify proteins that interact with JHAMT1 in the beetle","dates":{"publication":"Fri Jan 24 00:00:00 GMT 2025"},"accession":"PXD060186","cross_references":{"TAXONOMY":["561076"],"pubmed":["39822051","34571141"]}}