{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Chen Ding"],"species":["Homo Sapiens"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0011114000"],"submitter_email":["chend@fudan.edu.cn"],"submitter_affiliation":["Fudan University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["Human pluripotent stem cell-derived cardiovascular progenitor cells (hCVPCs) and cardiomyocytes (hCMs) possess therapeutic potential for infarcted hearts; however, their efficacy needs to be enhanced. Here we tested the hypotheses that the combination of decellularized porcine small intestinal submucosal extracellular matrix (SIS-ECM) with hCVPCs, hCMs, or dual of them (Mix, 1:1) could provide better therapeutic effects than the SIS alone, and dual hCVPCs with hCMs would exert synergic effects in cardiac repair. The data showed that the SIS patch well supported the growth of hCVPCs and hCMs. Epicardially implanted SIS-hCVPC, SIS-hCM, or SIS-Mix patches at 7-day post-myocardial infarction significantly ameliorated functional worsening, ventricular dilation and scar formation at 28- and 90-day post-implantation in C57/B6 mice, whereas the SIS only mildly improved function at 90-day post-implantation. Moreover, the SIS and SIS-cell patches improved vascularization and suppressed MI-induced cardiomyocyte hypertrophy and expression of <i>Col1</i> and <i>Col3</i>, but only the SIS-hCM and the SIS-Mix patches increased the ratio of collagen III/I fibers in the infarcted hearts. Further, the SIS-cell patches stimulated cardiomyocyte proliferation via paracrine action. Notably, the SIS-Mix had better improvements in cardiac function and structure, engraftments, and cardiomyocyte proliferation. Proteomic analysis showed distinct biological functions of exclusive proteins secreted from hCVPCs and hCMs, and more exclusive proteins secreted from co-cultivated hCVPCs and hCMs than mono-cells involving in various functional processes essential for infarct repair. These findings are the first to demonstrate the efficacy and mechanisms of mono- and dual-hCVPC- and hCM-seeding SIS-ECM for repair of infarcted hearts based on the side-by-side comparison."],"pubmed_title":["Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted hearts."],"pubmed_authors":["Jiang Yun Y, Zhang Ling-Ling LL, Zhang Fan F, Bi Wei W, Zhang Peng P, Yu Xiu-Jian XJ, Rao Sen-Le SL, Wang Shi-Hui SH, Li Qiang Q, Ding Chen C, Jin Yin Y, Liu Zhong-Min ZM, Yang Huang-Tian HT"],"pubmed_title_synonyms":["dTAF[[II]]230, TAF[[II]]250, d230, human being, Modern, TAF200, Matrix, Endogenous, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, TAF250/230, Matrices, EfW1, Cell, Human, dTAF230, ter, dmTAF[[II]]230, TAFII250, iPSC, Homo sapiens, dmTAF1, Taf230, Term, p230, TAF[[II]]250/230, TFIID, iPS cell, Extracellular Matrices, Man, TAF250, Taf[[II]]250, Regenerations, Taf200, matrisome, dTAF[[II]]250, TAF[[II]]230, Man (Taxonomy), TFIID TAF250, cel, cell, CG4216, Taf1p, TAF[II]250, CG17603, TAF[[II]], human, dTAF250, DmelCG4216, DmelCG17603, Endogenous Regeneration, Hearts., Taf250, SR3-5, Bsg75C, Modern Man, Extracellular, Regeneration, TAF, humans, TAF230, TAF1"],"description_synonyms":["liquid chromatography tandem mass spectroscopy, LC-MS2, DmelCG7212, DXS1357E, 6C6-AG, LCMSMS, sd-5, CDM, BAP31, Proteins, LC-MS/MS, number, Gene, imp13, LFQ., 3R23, Mischung, NAG22, LC-MS-MS, Protein Gene Products, L-CAD, Gene Proteins, LC/MS/MS, liquid chromatography-tandem mass spectroscopy, H-CAD, LC-MSMS, Protein, cardinality, proteomic analysis, Gene Products, liquid chromatography tandem mass spectrometry, CG7212, LCAD, Imp13, DDCH, CG 7212, HCAD"],"name_synonyms":["dTAF[[II]]230, TAF[[II]]250, d230, human being, Modern, TAF200, Matrix, Endogenous, l(3)84Ab, dTAFII250, BG:DS00004.13, TAFII-250, TAF250/230, Matrices, EfW1, Cell, Human, dTAF230, ter, dmTAF[[II]]230, TAFII250, iPSC, Homo sapiens, dmTAF1, Taf230, Term, p230, TAF[[II]]250/230, TFIID, iPS cell, Extracellular Matrices, Man, TAF250, Taf[[II]]250, Regenerations, Taf200, matrisome, dTAF[[II]]250, TAF[[II]]230, Man (Taxonomy), TFIID TAF250, cel, cell, CG4216, Taf1p, TAF[II]250, CG17603, TAF[[II]], human, dTAF250, DmelCG4216, DmelCG17603, Endogenous Regeneration, Hearts., Taf250, SR3-5, Bsg75C, Modern Man, Extracellular, Regeneration, TAF, humans, TAF230, TAF1"],"pubmed_abstract_synonyms":["Heart, , projections, Heart Muscle Cells, Needs, Collagens, Therapeutic Effects, Product, Strokes, infarction (MI), Laboratory, Myocyte, postnatal development, Mus domesticus, Mononucleosis, growth and development, CASP-14, House Mouse, C57 Black/6, Cardiac Muscle, dmTAF[[II]]230, C57, Generic Action, DmelCG4636, Biological, ATCOL1, Mother Cells, symptoms, 3, B6, Extracellular Matrices, cardiomyocyte, Zyderm, Biological Product, PDGF2, Effect, Muscle Cell, MIX, C57B/6, multicellular organismal biosynthetic process, treatment, S4, Cardiac infarction, single-organism biosynthetic process, Target Populations, Biologic Drugs, TFIID TAF250, myocardial infarction (disease), cel, Natural, anabolism, Cardiac Myocytes, Microfibril Collagen Hemostat, hypoplasia, Swiss Mice, mononucleosis, Biological Drugs, Stroke, Biological Medicine, GPIa*, l(2)k03107, glandular fever, Hearts., collagen, Cardiomyocytes, WAVE-1, Collagen Fleece, papilla, Therapeutic Use, disease management, Therapies, Medicine, Health Services Need, CG7776, Medicines, Gammaherpesviral mononucleosis, l(2)k13811, Collagen, Heart Attacks, Biologic Drug, Myocardial infarction, Therapy, screening, Infarct, dTAF[[II]]230, Scar, C57Bl, SCAR/WAVE, Biologic Products, Myocardial Infarcts, Infarcts, Needs and Demand, mouse, e(Pc), lamina, Need, TAF200, Myocardial Infarct, flanges, TAFII-250, DmSCAR, TAF250/230, CG4636, cardiocyte, L[[3]]CP3, Cicatrization, TAFII250, SCAR, Colony-Forming Unit, Stem Cell, PDGF-B, Cardiovascular Stroke, BEST:SD02991, Biopharmaceuticals, Myocardial infarction NOS, Mini-ICE, shelf, Biologic Product, NOS, scar, Therapeutic Effect, familial hypertrophic cardiomyopathy, DWave, SIS, Sis, Hypertrophies, Mother Cell, D-SCAR, MILD1, Mus musculus, Action, Populations, heart attack, DmelCG7776, Caspase-14 subunit p10, growth pattern, AI195380, Biological Medicines, non-developmental growth, mice, Stem, wave, F14F8.230, Biological Drug, shelves, Health Services Needs, cardiac myocyte proliferation, WAVE, Swiss Mouse, heart muscle cell proliferation, signs, Colony Forming Units, Caspase-14 subunit p19, Biologics, RPS4, MICE, CG17603, PDGF-2, TAF[[II]], projection, Treatments, sis, MYOCARDIAL INFARCTION, ridge, domesticus, infectious mononucleosis, Heart Muscle Cell, myocardial, pdgf2, Taf250, rps4, SR3-5, DmelLcp3, E(PC), BG:DS02740.11, anon-48Ac, Cells, Infarction of heart, Heart attack, heart muscle cell, Filatov's disease, Collagen Felt, dWAVE, Mouse, (MI), Dilation, CONSTANS-like 1, Infarction, TAF230, 3.4.22.-, IBGC5, GH05739, Dilatations, Pfeiffer's disease (disorder), d230, C57/B6, conformation, Biologic Pharmaceuticals, Effects, lamellae, Colony-Forming Units, SCR10, Progenitor Cell, Matrix, alpha-Collagen, Gene, mini-ICE, dTAFII250, biosynthesis, Matrices, EfW1, process of organ, C57Black6, lamella, Attack - heart, ccg2, Cardiac, Microfibril, Mono, III, reduced, GH14582, House, dmTAF1, Taf230, Dermodress, cardiac muscle fiber, Collastat, Wave, C57Bl\\6, Gene Products, Dscar, Mus musculus domesticus, Myocardial Infarction, Scars, CCG2, tiny, Mice, Progenitor, TAF250, Biologicals, DmelCG2043, Drugs, Gammaherpesviral mononucleosis (disorder), scr10, cardiomyocyte proliferation, Health Services, Taf200, matrisome, dTAF[[II]]250, lcp3, formation, Swiss, cell, LCP-3, Natural Product, Target, Avitene, Mother, Taf1p, ridges, Population, Muscle Cells, Scarring, alpha Collagen, CB57, synthesis, dTAF250, F14F8_230, Use, CP3, Infectious Mononucleosis, Pfeiffer's disease, Extracellular, ECM, Cardiac Myocyte, Heart Attack, TAF, laminae, Collagenfleece, relational structural quality, Biologic, Pharmaceuticals, small, Dilations, Products, Colony Forming Unit, TAF[[II]]250, MI - Myocardial infarction, myocardial infarction, LcpIII, findings, Biologic Medicines, C57/BL6, Progenitor Cells, MYOCARDIAL, AI838537, Proteins, Heart Muscle, HCM - hypertrophic cardiomyopathy, C57Black, l(3)84Ab, human pluripotent stem cell, Hearts, function, BG:DS00004.13, MI, Cell, EMILIN4, myocardial infarct, dTAF230, development, Mus, p230, cardiac muscle cell, Protein, proteomic analysis, Biopharmaceutical, Collagen Hemostat, C57Black/6, TAF[[II]]250/230, TFIID, Kissing disease, MIXL, Cardiomyocyte, LCP3, flange, Pangen, organ process, C57BL6, Taf[[II]]250, Uses, Cardiovascular, TAF[[II]]230, DMLCP3, underdeveloped, Myocardial Infarctions, l(2)28-28-12, dxs306, other collagen, postnatal growth, DXS306, Target Population, anon-35Fa, severe cutaneous adverse reaction, House Mice, Avicon, TAF[II]250, Glandular Fever, Cardiac Muscle Cells, Myocardial, Cardiac Muscle Cell, Laboratory Mice, Drug, Protein Gene Products, DmelCG5861, Gene Proteins, processes, DmelCG17603, Infarctions, Therapeutic, anon-35Fc, cardiac myocyte, Monocytic angina, Natural Products, monocytic angina, Treatment, INFARCTION (MI), CG2043, Glandular fever, SSV, MMRN, c-sis, growth, Cardiovascular Strokes, Laboratory Mouse, Myocardial infarction (disorder), TAF1"],"additional_accession":[]},"is_claimable":false,"name":"Dual human iPSC-derived cardiac lineage cell-seeding extracellular matrix patches promote regeneration and long-term repair of infarcted hearts","description":"The LC-MS/MS-based in-depth proteomic analysis of the secretory proteins was performed using the CdM collected from the equal number of hCVPCs, hCMs and their 1:1 mixture. Then the label-free quantification of MS data and statistical analyses were used to compare the three groups.","dates":{"publication":"Mon Feb 24 00:00:00 GMT 2025"},"accession":"PXD061158","cross_references":{"TAXONOMY":["9606"],"pubmed":["37274446"]}}