{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Yi Zhu"],"species":["Mus Musculus"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0011406000"],"submitter_email":["zhuyi@tmu.edu.cn"],"submitter_affiliation":["Tianjin Medical University"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<h4>Background</h4>The CEMIP (cell migration-inducing protein) exhibits extremely high expression levels in smooth muscle tissues. However, whether CEMIP modulates the contractile phenotype of vascular smooth muscle cells and confers blood pressure remains elusive.<h4>Methods</h4>To explore the role of CEMIP in SMCs, we generated a mouse model with SMC-specific CEMIP deficiency (SMC-<i>Cemip</i><sup><i>KO</i></sup>). By combining coimmunoprecipitation assay and molecular docking prediction, we identified MLC20 (myosin light chain 20) and its phosphatase, PP1c (protein phosphatase 1c) as binding proteins for CEMIP. To elucidate the mechanism by which CEMIP interacts with MLC20 and PP1c, bioluminescence resonance energy transfer assay and motif mutation were utilized to unravel the intricate network.<h4>Results</h4>CEMIP is abundantly expressed in smooth muscle tissues including the mesenteric artery, aorta, and intestine. In a serum-starved contractile phenotype, SMCs responded to serum stimulation by gradually decreasing CEMIP expression, as they shifted to a secretory phenotype. The mice lacking CEMIP in SMCs showed significantly reduced contractility and blood pressure compared with their counterparts of age-matched wild-type littermates (<i>Cemip</i><sup><i>flox/flox</i></sup>). Mechanistically, we demonstrated that CEMIP directly interacted with MLC20 to maintain its phosphorylation. In addition, CEMIP sequestered MLC20 from its phosphatase, PP1c, without affecting the kinase MLCK (myosin light chain kinase). Moreover, CEMIP contains 3 critical RVxF (consensus sequence RxxQV/I/LK/RxY/W) motifs that are responsible for binding to PP1c. Mutations in these motifs restored the interaction between PP1c and MLC20.<h4>Conclusions</h4>CEMIP functions as a gatekeeper for maintaining the contractile phenotype of SMCs via the PP1c-MLC20 cascade. CEMIP is indispensable for maintaining blood pressure."],"pubmed_title":["CEMIP Maintains Vascular Contractility by Controlling PP1c-MLC20 Cascade in SMCs."],"pubmed_authors":["Yuan Ze Z, Li Qi Q, Bai Xue X, Zhang Kai K, He Jinlong J, Ai Ding D, Cui Qinghua Q, Zhu Yi Y, Li Bochuan B"],"additional_accession":[]},"is_claimable":false,"name":"SMC-CemipKO aortic media immunoprecipitated with anti-MLC20 antibodies for proteomic","description":"Protein was extracted from the aortic media of 8-week-old SMC-CemipKO and Cemipflox/flox mice. Whole-cell lysates were immunoprecipitated with anti-MLC20 antibodies for proteomic analysis. Mass spectrometry identified top 10 MLC20-binding proteins as listed.","dates":{"publication":"Tue Mar 18 00:00:00 GMT 2025"},"accession":"PXD062008","cross_references":{"TAXONOMY":["10090"],"pubmed":["40590126"]}}