{"database":"iProX","file_versions":[],"scores":null,"additional":{"omics_type":["Proteomics"],"submitter":["Ying Yan"],"species":["Mus <genus>"],"full_dataset_link":["http://www.iprox.org/page/project.html?id=IPX0011730000"],"submitter_email":["yanyingdoctor@sina.com"],"submitter_affiliation":["General Hospital of Northern Theater Command"],"sample_protocol":[""],"repository":["iProX"],"data_protocol":[""],"pubmed_abstract":["<h4>Background</h4>Radiation enteritis (RE) is an unavoidable complication during radiotherapy for pelvic malignancies, characterized by chronic inflammation, fibrosis, and vascular injury in the intestinal tissue. Currently, there is a lack of research that delves into the relationship between inflammatory factors and key proteins in RE.<h4>Methods</h4>This study employed high-throughput proteomics to analyze intestinal tissues from RE rats and healthy controls, identifying differentially expressed key proteins. The degree of intestinal damage was validated through HE staining. Furthermore, five Mendelian randomization methods were used to analyze the causal relationship between 70 serum circulating inflammatory factors and CD13 levels. Sensitivity analyses, including heterogeneity tests, leave-one-out tests, and horizontal pleiotropy tests, were performed to ensure the robustness and reliability of the results.<h4>Results</h4>CD13 was identified as a key differentially expressed protein, with its expression significantly upregulated in RE rats and positively correlated with disease severity. Bidirectional Mendelian randomization analysis revealed causal relationships between CD13 and four inflammatory factors: increased levels of CCL28 and EN-RAGE may promote the rise in CD13, while increased levels of TAM-binding protein may be associated with decreased CD13 levels. Additionally, higher CD13 levels were found to be associated with increased levels of interleukin-12. Sensitivity analyses indicated good consistency and reliability in terms of heterogeneity and pleiotropy for these exposure variables.<h4>Conclusion</h4>This study reveals the potential mechanistic role of CD13 in RE. Moreover, the identified CD13-associated inflammatory factors offer potential targets for the development of new prevention and treatment strategies, with significant clinical implications."],"pubmed_title":["Exploring the role of CD13 and inflammatory factors in radiation enteritis: insights from high-throughput proteomics and Mendelian randomization analysis."],"pubmed_authors":["Ren Xue X, Shang Feng F, Yang Defu D, Xu Ying Y, Yan Ying Y"],"name_synonyms":["KZP, mAPN, Radiations, Cognitive Function, Alanyl aminopeptidase, Mendelian Randomization., enteritis, Peptidomics, GP150, Aminopeptidase M, Function, PEPN, P150, membrane alanyl aminopeptidase activity, Insight, Cognitive, CD13, Microsomal aminopeptidase, Concept, Cognitive Functions, Insights, Cognitions, Role Concept, Kidney Zn peptidase, Roles, Functions, Membrane protein p161, 3.4.11.2, Role Concepts, AP-N, Role, Concepts, AP-M, LAP1, Analysis, inflammatory disorder of small intestine, rAPN, Cd13, Apn, Enteritides, APN"],"pubmed_abstract_synonyms":["Rat, Alanyl aminopeptidase, Vascular Injuries, BG:DS00941.6, Inflammations, degree (angle), Activity, single-organism developmental process, Laboratory, Blood, postnatal development, Radiation Therapies, Norway Rat, cDNA 2, Interleukin-12 p70, Edodekin Alfa, MtPolA, growth and development, protein, Tumor, Serum, P150, IL 12, Norway Rats, CG9729, prevention, MAGE-E1 antigen, Techniques, rat, DSmurf, Roles, Method, System Injuries, AP-N, ENC-1, AP-M, Concepts, 2, Norway rat, LAP1, transient, DZO-1, Analysis, Research Activity, CG9731, protein aggregate, EN-RAGE, Laboratory Research, prevention and control, IKKg, pol gamma, KEY, Key, Transport Proteins, Priorities, mAPN, CAAF1, treatment, dzo-1, inflammatory response, Tissue, Radiotherapies, procedures, MRP-6, DYP, CD13, Laboratory Rat, AAC47658, allergic reaction, preventive measures, GOK, System Injury, Methodological Studies, HCA1, pol gamma-alpha, Smurf, Severity of Illness, Role Concepts, Therapies, CG8987, Malignancies, Research Priority, TARDY ASYNCHRONOUS MEIOSIS, rAPN, Cd13, CG4943, Histological Labeling, Tumors, D-smurf, Therapy, CG11962, X-ray therapy, PIG10, CT36877, CYCA1, preventive therapy, Radiation, Neutrophil S100 protein, l(2)brS, enteritis, Targeted Radiation, NRPB, Research Priorities, Labeling and Staining, PEPN, TAM1, Rattus, Lack, Procedure, Blood Serum, Carrier, DmIKKgamma, Mus norvegicus, l(2)br5, IL12, Benign, Role Concept, Rattus norwegicus, dIKK, Radiotherapy, DAMAGE, Hepatocellular carcinoma-associated protein 1, Kenny, Role, Cirrhosis, simple tissue, Pch, inflammatory disorder of small intestine, CG31349, Binding Protein, S100 calcium-binding protein A12, Research and Development, KZP, DmelCG8987, DmelCG4943, DNApol-gamma, IKK-gamma, Benign Neoplasms, radioisotope therapy, Methodological, 34Dc, Methodological Study, rats, TP53I10, Treatments, Microsomal aminopeptidase, Malignant Neoplasms, Activities, Calcitermin, SCYA28, DmelCG16910, Labelings, Vascular Injury, CG9763, Specificity and Sensitivity, Severity, Illness Severities, Migration inhibitory factor-related protein 6, Innate, mMage-e1, Binding Proteins, DmelCG43140, CCL28, polgamma, Radiation Treatments, Inflammatory Response, KLHL37, Carrier Protein, Targeted Radiation Therapy, Procedures, norvegicus, Peptidomics, KLHL35, Calcium-binding protein in amniotic fluid 1, mtDNA pol, Neoplasms, dSmurf1, GP150, D-pol gamma-alpha, Histological Labelings, Benign Neoplasm, Gene, l(2)34Dc, protein-containing complex, Malignant, dIKK-gamma, Calgranulin-C, DNApol-gamma125, Rattus sp. strain Wistar, Rats, Kidney Zn peptidase, Interleukin 12, Vascular, sensitive, IL-12, DmIKK-gamma, Gene Products, Studies, Histological, Fibroses, dmIKKgamma, Rattus rattiscus, xvt, IKK[[gamma]], Technique, sensitivity, Enteritides, brown rat, F2P24.10, study, D11S4896E, Injury, Rattus norvegicus, Staining, Malignancy, Research, mtPolA, inflammation, Targeted Radiotherapies, AI847422, Pyd/ZO-1, Pyd, Interleukin 12 p70, Cytotoxic Lymphocyte Maturation Factor, CG11782, Vascular System, Neoplasias, Study, d-smurf, IKK, Innate Inflammatory Response, Membrane protein p161, pyd(Z01), Mst, Chronic, Sensitivity, Development and Research, Targeted Radiotherapy, Vascular System Injury, polgamma-alpha, Cancer, Rattus norvegicus8, F2P24_10, Dmel_CG31349, Illness Severity, Tam, TAM, leukemia, Disease, Malignant Neoplasm, Targeted Radiation Therapies, protein complex, Aminopeptidase M, Proteins, Alpha-dystrobrevin-associated MAGE Protein, Targeted, Random, Stainings, IMD10, Natural Killer Cell Stimulatory Factor, membrane alanyl aminopeptidase activity, Dsmurf, Labeling, ZO1, CYCLIN A1, Concept, tam, dSmurf, development, IKKgamma, Dmel_CG9731, CAGC, disease management., Priority, native protein, Mendelian Randomization, Tamou, CG12409, Smurf ubiquitin ligase, Laboratory Rats, Protein, Extracellular newly identified RAGE-binding protein, Research Activities, Neoplasm, ZO-1, Disease Severities, techniques, radiation therapy, CGRP, Radiations, Transport Protein, p6, RGD1560259, Dmikkgamma, Polgamma, prophylaxis, postnatal growth, Randomization, Specificity, myeloproliferative syndrome, Allocation, STRMK, Radiation Treatment, Cancers, arc degree, CG16910, l(2)br14, Injuries, Protein Gene Products, Gene Proteins, br5, Therapeutic, control, anon-34Dc, Innate Inflammatory Responses, 3.4.11.2, Radiation Therapy, Treatment, CG43140, Norway, IL-12 p70, Disease Severity, growth, Neoplasia, Serums, methodology, Apn, Gunn rats, APN"],"pubmed_title_synonyms":["KZP, mAPN, Radiations, Cognitive Function, Alanyl aminopeptidase, Mendelian Randomization., enteritis, Peptidomics, GP150, Aminopeptidase M, Function, PEPN, P150, membrane alanyl aminopeptidase activity, Insight, Cognitive, CD13, Microsomal aminopeptidase, Concept, Cognitive Functions, Insights, Cognitions, Role Concept, Kidney Zn peptidase, Roles, Functions, Membrane protein p161, 3.4.11.2, Role Concepts, AP-N, Role, Concepts, AP-M, LAP1, Analysis, inflammatory disorder of small intestine, rAPN, Cd13, Apn, Enteritides, APN"],"description_synonyms":["Water, Rat, Raw, Laboratory, Ray, Grenz, Norway Rats, prevention, Experimental Uses, Sprague-Dawley Rats, 5730420M11Rik, Small, rat, Method, Parafilm, echotomography, 4, Software Engineering, Analysis, 7, Work Flow, 9, integumentum commune, Animalia, SET, Divorced, Analyses, Tissue, AI844555, entire skin, Hemotoxylin, Divorces, X, T6G21.3, SURGICAL AND MEDICAL PROCEDURES, AI047805, Rubberplants, DmelCG4299, set, anterior intestine, X Rays, ultrasonic diagnosis, Fam91a1, Otf11, 10(6H)-pentol, AV220772, skin and subcutaneous tissue, Histological Labeling, preventive therapy, Stars, Radiation, animalia, Dipotassium Salt, Procedure, Spectrum Analysis, Software Tools, Computer Applications, TPSG1, X Ray, Workflows, Rattus norwegicus, Dm1, Computer Applications Software, 11b-dihydro-, simple tissue, Industrial, Software Applications, Industrial Arts, whole organism, HLA-DR-associated protein II, DI-2, maleate, Source Software, I-2Dm, disodium salt, Spectrometry, Methodological, study protocol, rats, 2-d)pyran-3, I-2PP1, Applications, Sodium Salt, TAF-IBETA, Xrays, Koerper, PRSS31, Indicator, TAF-Ibeta, entire integument, STARS, TMT, Xray, Work Flows, Eosine Yellowish-(YS), Computer Software Applications, region of skin, X-ray image, Peptidomics, Otf-11, number, Hematoxiline, Eosine Yellowish, Experimentation, protein-containing complex, thomson, CG7826, mass-to-charge ratio, Rats, Figs, Gene Products, CG7835, CG42273, Animal Experimental Uses, 7'-tetrabromo-3', Technique, Application, Skn-1a, Acid Red 87, X-Radiation, Open Source Softwares, DmelCG7615, anatomical systems, skin, radioisotope scanning, interventionDescription, Software Application, Hydroxybrasilin, Open Source Software, 2pp2a, SKIN, CG10574, CD156, rats <Rattus>, Computer Software Application, Study, skin organ, 2PP2A, Tools, radiography, dSET, dSet, X-ray diagnosis, Eosin Y, Potassium, ultrasonography, Oct-11a, grupo, body, Rays, Proteins, Haematoxylon, skin zone, whole body, Stainings, Engine, Tool, General Hospital, skin plus hypodermis, Research Ethics, Experiment, MS, native protein, C.I. Acid Red 87, I-2PP2A, Laboratory Rats, Dm I-2, chemical analysis, Skin, Mass Spectrum Analyses, skin benign neoplasm, Intervention, AU020952, Col4a-1, ensemble, Protein., prophylaxis, Separations, Striated muscle activator of Rho-dependent signaling, Benz(b)indeno(1, CD156a, X-ray, ME-IV, Epoc-1, male human body, plan specification, Computer Programs, Gene Proteins, Applications Softwares, control, Stress, CG7615, X-Radiations, Norway, X-Ray, Holtzman, the integument, IPP2A2, Bru, pelt, determination, Skn-li, Sprague Dawley Rats, Norway Rat, protein, Techniques, Tier, X Radiation, Mammals, Min, Norway rat, 2', Grenz Rays, protein aggregate, (2Z)-but-2-enedioate, small intestine, prevention and control, Computer Program, DmelCG42273, male, PIST, Mass Spectrum Analysis, Group, Svc, Eosine, reference sample, TAF-I, Experiments, Open, E430039A18Rik, dermoid system, min, Computer Programs and Programming, mAPC, Groups, DEPP, Laboratory Rat, preventive measures, Intestines, Grenz Ray, IGAAD, Methodological Studies, Rubberplant, 6'-dihydroxy-, DmelCG10574, Holtzman Rat, Fseg, 4', radionuclide imaging, Reagents, Animal Experiments, Small Intestine, phapii, Holtzman Rats, Water Stress, Hydroxybrazilin, Arts, Sprague-Dawley, Dmel_CG7826, 5', StF-IT-1, Th, Labeling and Staining, Rattus, integumental organ, Search, Source Softwares, Programs, Spectroscopy, Program, Fasting-induced gene protein, Mus norvegicus, S-adenosyl-L-methionine:thiol S-methyltransferase activity, BC033609, Computer Applications Softwares, Softwares, General, Animal Research, GOPC1, Dmel_CG7835, Mnb, MNB, MS/MS, Control, Sprague Dawley, CG4299, AW124434, Eosin (yellowish) (free acid), Methodological Study, mKIAA0493, intestinum tenue, vertebrate epidermis, Labelings, 6a, FAIMS, dermal system, i2pp2a, Gruppe, dermis plus epidermis plus hypodermis, norvegicus, Procedures, Reagent, Histological Labelings, Gene, Tetrabromofluorescein, Spectrum Analyses, Computer, 2210402P09Rik, presence, PHAPII, epidermis, Intervention or Procedure, Rattus sp. strain Wistar, method, Ms1, cis-(+)-, method used in an experiment, General Hospitals, Roentgen Rays, Studies, Mass, Histological, Del(8)44H, Rattus rattiscus, Animal, roentgenography, Separated, Reagents and Indicators, Mass Spectroscopy, brown rat, Sprague-Dawley rat, study, Search Engines, mid intestine, Rattus norvegicus, Staining, MS1, Research, MS2, ipp2a2, Interventional, metazoa, labeling, Indicators, Sprague-Dawley Rat, Animal Experimental Use, Spiro(isobenzofuran-1(3H), grupos, taf-ibeta, male sterility 1, Control Group, thiol methyltransferase activity, Controlled, Rattus norvegicus8, Applications Software, Decidual protein induced by progesterone, Intervention Strategies, Open Source, Controlling, DYRK1, Animal Experiment, Computer Software, protein complex, Males, Sprague Dawley Rat, igaad, Hospital, Labeling, group, Roentgen Ray, count in organism, echography, Software Tool, Oct11, skin region, Protein, Metazoa, CAL, I2PP2A, Roentgen, Dyrk1, Skin-1a, tegument, Software, tandem MS, Data Base, Radiations, Mass Spectrum, Separation, MALE STERILITY 1 PROTEIN, Eosin, CC1, Engineering, Sprague-Dawley rats, Rest, vertebrate integument, Small Intestines, small bowel, dJ94G16.2, 9'-(9H)xanthen)-3-one, Protein Gene Products, Experimental Use, portion of skin, dSET/TAF-Ibeta, FIG, Fig, 2610030F17Rik, integument, rat <Rattus>, ultrasound, cardinality, assay, AA407739, groupe, Gunn rats"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the Role of CD13 and Inflammatory Factors in Radiation Enteritis: Insights from High-Throughput Proteomics and Mendelian Randomization Analysis","description":"This study first compares the differences in protein expression profiles of small intestine tissues between RE rats and control rats using high-throughput proteomics technology. A total of 20 male Sprague-Dawley rats are randomly divided into two groups: the control group and the radiation group, with 10 rats in each group. The radiation group is subjected to the previously established RE model, receiving a single 10 Gy dose of 6MV X-ray irradiation at a dose rate of 220 cGy/min, with a source-to-skin distance of 100 cm (Fig.2). The experimental protocol has been approved by the Animal Experimentation Committee of the Northern Theater General Hospital, with approval number 2024-56 from the Animal Medical Research Ethics Subcommittee, dated August 28, 2024. During the design and implementation phases, we strictly adhered to the \"3Rs\" principle. At 72 hours post-irradiation, rats are euthanized and small intestine tissue samples are collected. The tissues are fixed, dehydrated, and embedded in paraffin, then sectioned into 5 μm thick slices for HE staining. The staining procedure involves hematoxylin staining for 5-10 minutes, eosin staining for 1-2 minutes, followed by dehydration, clearing, and mounting. Total protein was extracted from small intestine tissues and analyzed using TMT labeling quantitative proteomics technology. After processing, the samples were labeled with TMT reagents and separated and detected using the EASY-nLC™ 1200 nanoflow UHPLC system and Orbitrap Exploris 480 mass spectrometer (equipped with FAIMS). The mass spectrometry parameters were set to a resolution of 60,000 for MS1 and 30,000 for MS2 (at 200 m/z) to ensure high-quality data acquisition. All experiments were performed in triplicate to ensure the reliability of the results. The raw mass spectrometry data were processed using Proteome Discoverer software (version 2.4, Thermo Fisher Scientific) and searched against the Uniprot database (rattus_norvegicus_uniprot_2021_7_15. fasta, containing 36,213 sequences) using the Sequest HT search engine. Differential expression analysis was conducted with Fold Change (FC) ≥2.0 for upregulated proteins and FC ≤0.50 for downregulated proteins, with a significance level of p<0.05. This workflow enabled accurate identification and quantification of proteins in the samples and allowed us to pinpoint proteins with significant differential expression under different conditions","dates":{"publication":"Fri Apr 18 00:00:00 GMT+01:00 2025"},"accession":"PXD063093","cross_references":{"TAXONOMY":["10088"],"pubmed":["40332653"]}}