iProXProteomicsYongliang ZhangNicotiana Benthamianahttp://www.iprox.org/page/project.html?id=IPX0012273000cauzhangyl@cau.edu.cnChina Agricultural UniversityiProXfalseProximity Profiling of Chloroplast–Endoplasmic Reticulum Membrane Contact Sites in Nicotiana benthamiana Using Split-TurboID During Viral InfectionWe fused the N-terminus of TurboID to the chloroplast outer membrane protein OEP7 and the C-terminus to the endoplasmic reticulum membrane protein RTNLB2. When the chloroplast and endoplasmic reticulum come into close proximity to form membrane contact sites (MCSs), the split-TurboID is reconstituted into an active proximity-labeling enzyme that biotinylates nearby proteins. In this proteomic study, Barley stripe mosaic virus (BSMV) infection was used as the experimental variable.Fri Jun 13 00:00:00 BST 2025PXD0649674100